A5025799390- SARS-CoV-2 and COVID-19 Research
- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Hepatitis B Virus Studies
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Hepatitis Viruses Studies and Epidemiology
- Liver Disease Diagnosis and Treatment
- COVID-19 Clinical Research Studies
- Animal Virus Infections Studies
- HIV, Drug Use, Sexual Risk
- Biosensors and Analytical Detection
- Viral Infections and Immunology Research
- Vaccine Coverage and Hesitancy
- Parvovirus B19 Infection Studies
- Liver Disease and Transplantation
- CRISPR and Genetic Engineering
- Molecular Biology Techniques and Applications
- Pneumocystis jirovecii pneumonia detection and treatment
- Virus-based gene therapy research
- Cytomegalovirus and herpesvirus research
- vaccines and immunoinformatics approaches
- Animal Disease Management and Epidemiology
Sheba Medical Center
2016-2025
Israel Ministry of Health
2016-2025
Tel Aviv University
2016-2025
University of Oxford
2021
Ministry of Environmental Protection
2017
University of Cologne
2016
Ben-Gurion University of the Negev
2016
Ministry of Health of the Russian Federation
2013
Quark Pharmaceuticals (United States)
2001-2005
Weizmann Institute of Science
2004
Hypoxia is an important factor that elicits numerous physiological and pathological responses. One of the major gene expression programs triggered by hypoxia mediated through hypoxia-responsive transcription hypoxia-inducible 1 (HIF-1). Here, we report identification cloning a novel HIF-1-responsive gene, designated RTP801. Its strong up-regulation was detected both in vitro vivo animal model ischemic stroke. When induced from tetracycline-repressible promoter, RTP801 protected MCF7 PC12...
<h2>Summary</h2><h3>Background</h3> Antiretroviral therapy (ART) is crucial for controlling HIV-1 infection through wide-scale treatment as prevention and pre-exposure prophylaxis (PrEP). Potent tenofovir disoproxil fumarate-containing regimens are increasingly used to treat prevent HIV, although few data exist frequency risk factors of acquired drug resistance in regions hardest hit by the HIV pandemic. We aimed do a global assessment after virological failure with first-line...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
<ns3:p>Late in 2020, two genetically-distinct clusters of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) with mutations biological concern were reported, one the United Kingdom and South Africa. Using a combination data from routine surveillance, genomic sequencing international travel we track dispersal lineages B.1.1.7 B.1.351 (variant 501Y-V2). We account for potential biases surveillance efforts by including passenger volumes location where lineage was first London Africa...
The immunogenicity and safety of the Pfizer-BioNTech BNT162b2 mRNA vaccine in people living with human immunodeficiency virus type 1 (PLWH) are unknown. We aimed to assess this PLWH.In prospective open study, we enrolled 143 PLWH, aged ≥18 years, who attended our clinic 261 immunocompetent health-care workers (HCWs). Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain (RBD) IgG neutralizing antibodies were measured. Adverse events, viral load CD4 cell counts...
SARS-CoV-2 Delta (B.1.617.2) variant of concern (VOC) and other VOCs are spreading in Europe. Micro-neutralisation assays with sera obtained after Comirnaty (BNT162b2, BioNTech/Pfizer) vaccination 36 healthcare workers (31 female) demonstrated significant fold change reduction neutralising titres compared the original virus: Gamma (P.1) 2.3, Beta (B.1.351) 10.4, 2.1 2.6. The Alpha (B.1.1.7) was not significant. Despite being lower, remaining neutralisation capacity conferred by against is...
Investigation of SARS-CoV-2 spread and identification variants in sewers has been demonstrated to accurately detect prevalence viral strains is advantageous clinical sampling population catchment size. Herein, we utilized an established nationwide system wastewater concentration approaches perform large-scale surveillance nine different locations across Israel that were sampled from August 2020 February 2021 sequenced (n = 58). Viral sequences obtained the samples had high coverages genome,...
Background. Numerous studies have shown that baseline drug resistance patterns may influence the outcome of antiretroviral therapy. Therefore, guidelines recommend testing to guide choice initial regimen. In addition optimizing individual patient management, these data enable transmitted (TDR) be surveyed for public health purposes. The SPREAD program systematically collects gain insight into TDR occurring in Europe since 2001.
"Ampicillin rash," a phenomenon unique to patients with Epstein-Barr virus acute infectious mononucleosis (AIM) treated ampicillin, was first reported in the 1960s. The incidence estimated as being between 80% and 100%, figures have not been reviewed since those accounts. We sought establish current of rash associated antibiotic treatment among children AIM.A retrospective study all hospitalized diagnosed having AIM based upon positive serology 2 pediatric tertiary medical centers Israel.Of...
Genomic sequencing provides critical information to track the evolution and spread of SARS-CoV-2, optimize molecular tests, treatments vaccines, guide public health responses. To investigate spatiotemporal heterogeneity in global SARS-CoV-2 genomic surveillance, we estimated impact intensity turnaround times (TAT) on variant detection 167 countries. Most countries submit genomes >21 days after sample collection, 77% low middle income sequenced <0.5% their cases. We found that at least 0.5%...
Abstract One of the key questions regarding COVID19 vaccines is whether they can reduce viral shedding. To date, Israel vaccinated substantial parts adult population, which enables extracting real world signals. The vaccination rollout started on Dec 20th 2020, utilized mainly BNT162b2 vaccine, and focused individuals who are 60 years or older. By now, more than 75% this age group have been at least 14 days after first dose, compared to 25% between ages 40-60 old. Here, we traced Ct value...
A nosocomial outbreak of SARS-CoV-2 Delta variant infected 42 patients, staff and family members; 39 were fully vaccinated. The attack rate was 10.6% (16/151) among exposed reached 23.7% (23/97) patients in a highly vaccinated population, 16-26 weeks after vaccination (median: 25 weeks). All cases linked traced to one patient. Several transmissions occurred between individuals wearing face masks. Fourteen 23 became severely sick or died, raising question about possible waning immunity.
This study presents an analysis of the epidemiological trends parvovirus B19 (B19V) in Israel from 2010 to 2023, with particular emphasis on outbreak 2023. The utilized molecular diagnostic data individual patients obtained at Central Virology Laboratory. Between and 2022, 8.5% PCR-tested samples were positive for B19V, whereas this percentage surged 31% samples. Throughout period, annual cycles consistently peaked early spring/summer, most recent prominent occurring 2016. Predominantly,...
Integrase strand transfer inhibitors (INSTIs) are expected to be widely adopted globally, requiring surveillance of resistance emergence and transmission.We therefore sought develop a standardized list INSTI-resistance mutations suitable for the transmitted INSTI resistance.To characterize suitability HIV-1 drug (TDR) surveillance, we classified them according their presence on published expert lists, conservation in INSTI-naive persons, frequency INSTI-treated persons contribution reduced...
HCV affects 185 million people worldwide and leads to death morbidities. has a high genetic diversity is classified into seven genotypes 67 subtypes. Novel anti-HCV drugs (Direct-Acting-Antivirals) eligibility, resistance cure rates depend on geno/subtype (GT).Analysis of epidemiological information viral GT from patients undergoing genotyping in 2011-2015.Anonymized 52 centers was analyzed retrospectively.37,839 samples were included the study. We show that distribution similar throughout...
The COVID-19 pandemic and the fast global spread of disease resulted in unprecedented decline world trade travel. A critical priority is, therefore, to quickly develop serological diagnostic capacity identify individuals with past exposure SARS-CoV-2. In this study serum samples obtained from 309 persons infected by SARS-CoV-2 324 healthy, uninfected as well 7 patients 4-7 each ranging between 1-92 days post first positive PCR were tested an "in house" ELISA which detects IgM, IgA IgG...
•An international cohort of 3,355 patients with hepatitis C from 22 countries was evaluated for drug resistance following DAA therapy.•Nearly all harbored drug-resistant variants after treatment failure, over 2/3 having against ≥2 classes.•Pathways to included enrichment highly resistant and selection multiple variants.•Previously unrecognized in who failed NS5A inhibitors were identified at high frequencies.•Resistance frequent older people cirrhosis those infected genotypes 1b 4 failure....
Objective To quantify and characterise missed opportunities for earlier HIV diagnosis in patients diagnosed with advanced HIV. Design A retrospective observational cohort study. Setting central tertiary medical centre Israel. Measures The proportion of HIV, the to diagnose them earlier, rate clinical indicator diseases (CIDs) those patients. Results Between 2010 2015, 356 were 118 (33.4%) late, 57 (16%) disease. Old age (OR=1.45 (95% CI 1.16 1.74)) being heterosexual (OR=2.65 1.21 5.78))...
Abstract Introduction : Transmitted drug‐resistance mutations (TDRM) may hamper successful anti‐HIV‐1 therapy and impact future control of the HIV‐1 epidemic. Recently infected, therapy‐naïve individuals are best suited for surveillance such TDRM. In this study, TDRM, detected by next‐generation sequencing (NGS) were compared to those identified Sanger‐based population (SBS) in recently infected patients. Methods Historical samples from 80 patients, diagnosed between 2000 2014, analysed...
HIV-1 RNA monitoring, both before and during antiretroviral therapy, is an integral part of HIV management worldwide. Measurements viral loads are expected to assess the copy numbers all common subtypes accurately be equally sensitive at different loads. In this study, we compared for first time performance NucliSens v2.0, RealTime HIV-1, Aptima Quant Dx, Xpert load assays. Plasma samples (n = 404) were selected on basis their v2.0 results subtypes. Concordance, linear regression,...
HBsAg immune-escape mutations can favor HBV-transmission also in vaccinated individuals, promote immunosuppression-driven HBV-reactivation, and increase fitness of drug-resistant strains. Stop-codons enhance HBV oncogenic-properties. Furthermore, as a consequence the overlapping structure genome, some or stop-codons derive from drug-resistance RT. This study is aimed at gaining insight prevalence characteristics immune-associated escape mutations, chronically HBV-infected patients...
The BNT162b2 messenger RNA (mRNA) vaccine against severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) has been shown to be safe and effective in immunocompetent patients. safety efficacy of this liver transplantation (LT) recipients is still under evaluation. objective study was assess the among transplant recipients. immune responses 76 LT receiving doses were compared with those 174 age‐matched controls. Postvaccination immunoglobulin G (IgG) antibodies receptor‐binding domain...
Mutations in the p53 gene are most common genetic alterations found human malignancies. In this study, a search was done for mutations conserved regions of malignant mesotheliomas. Thirteen mesotheliomas collected over preceding 12 yr and maintained paraffin blocks were examined by polymerase chain reaction (PCR) followed both single-strand conformation polymorphism (SSCP) denaturing gradient-gel electrophoresis (DGGE). Direct sequence analysis performed cases suspected mutations. No exons 5...