Eukaryotic cells distinguish their chromosome ends from accidental DNA double-strand breaks (DSBs) by packaging them into protective structures called telomeres that prevent repair/recombination activities. Here we investigate the role of key telomeric proteins in protecting budding yeast degradation. We show Saccharomyces cerevisiae shelterin-like Rif1, Rif2, and Rap1 inhibit nucleolytic processing at both de novo native during G1 G2 cell cycle phases, with Rif2 showing strongest effects....

Abstract Social living animals need to recognize the presence of conspecifics in environment order engage adaptive social interactions. cues can be detected through different sensory modalities, including vision. Two main visual features convey information about conspecifics: body form and biological motion (BM). Given role that oxytocin plays behavior regulation across vertebrates, particularly salience reward values stimuli, we hypothesized it may also involved modulation perceptual...

Individuals in a population respond differently to stressful situations. While resilient individuals recover efficiently, others are susceptible the same stressors. However, it remains challenging determine if resilience is established as trait during development or acquired later life. Using behavioral paradigm zebrafish larvae, we show that stable and heritable trait, which determined exhibited early Resilient larvae unique stress-induced transcriptional response, with mutations...

Abstract The current restrictive criteria for gasotransmitters exclude oxygen (O 2 ) as a gasotransmitter in vertebrates. In this manuscript, I propose revision of to include O per se signaling molecule and 'essential gasotransmitter' This would enable us search protein‐based ‐binding sensors (gasoreceptors) all cells the brain or other tissues rather than specialized such carotid body gills. If microorganisms have gasoreceptors DosP FixL FNR with diverse domains, then eukaryotic must also...

Sociality is a complex phenomenon that involves the individual´s motivation to approach their conspecifics, along with social cognitive functions enable individuals interact and survive. The nonapeptide oxytocin (OXT) known regulate sociality in many species. However, role of OXT specific aspects still not well understood. In present study, we investigated contribution receptor (OXTR) signalling two different zebrafish behaviour: preference, by measuring shoal recognition, ability...

Telomere integrity in budding yeast depends on the CST (Cdc13-Stn1-Ten1) and shelterin-like (Rap1-Rif1-Rif2) complexes, which are thought to act independently from each other. Here we show that a specific functional interaction indeed exists among components of two complexes. In particular, unlike RIF2 deletion, lack Rif1 is lethal for stn1ΔC cells causes dramatic reduction viability cdc13-1 cdc13-5 mutants. This synthetic between complex occurs rif1Δ-induced alterations telomere length....

Abstract Animal‐based research and drug safety studies are essential to understanding the mysteries of nature long‐term survival humans. Due rapid increase in global human population, conflict‐ economically driven migration, tourism‐related activities, densely populated metropolitan areas, local policies, humans will be affected by a multitude novel disease‐causing microorganisms civilizational diseases. Despite disparities among countries, recent planned changes regulations concerning...

Abstract Sugars are one of the major metabolites and essential for nucleic acid synthesis energy production. In addition, sugars can act as signaling molecules. To study sugar at systemic level, there is an urgent need to systematically identify sugar‐sensing proteins acids. I propose terms “swodkoreceptor” “swodkocrine signaling,” derived from Polish word “słodki” meaning “sweet,” comprise all events, respectively, regardless their cellular location domains. This proposal intended...

The regulation of neuropeptide level at the site release is essential for proper neurophysiological functions. We focused on a prominent neuropeptide, oxytocin (OXT) in zebrafish as an vivo model to visualize and quantify OXT content resolution single synapse. found that OXT-loaded synapses were enriched with polymerized actin. Perturbation actin filaments by either cytochalasin-D or conditional Cofilin expression resulted decreased synaptic levels. Genetic loss robo2 slit3 displayed mutants...

The impact of gasocrine signaling towards human survival is currently unclear. I will highlight a few examples and endogasocrine how it affects the economy.

Understanding how cells sense gases or gaseous solutes is a fundamental question in biology and pivotal for the evolution of molecular organismal life. In numerous organisms, can diffuse into cells, be transported, generated, sensed. Controlling cellular environment essential to prevent damage due interactions with gas-dependent free radicals. Consequently, mechanisms governing acute gas sensing are evolutionarily conserved have been experimentally elucidated various organisms. However,...

Understanding how cells sense temperature is a fundamental question in biology and pivotal for the evolution of life. In numerous organisms, not only sensed but also generated due to cellular processes. Consequently, mechanisms governing sensation various organisms have been experimentally elucidated. Extending upon others' proposals demonstration protein- nucleic acid-based thermosensors, utilizing colonial India 'punkah-wallahs' analogy, I present my rationale necessity sensing every...

Oxytocin-like peptides have been implicated in the regulation of a wide range social behaviors across taxa. On other hand, environment, which is composed conspecifics that may vary their genotypes, also influences behavior, creating possibility for indirect genetic effects. Here, we used zebrafish oxytocin receptor knockout line to investigate how genotypic composition environment (G s ) interacts with genotype focal individual i its behavior. For this purpose, raised wild-type or knock-out...

In cellular signaling, a cell can send specific signals to another with clear intention. The sent are sensed by the receiving cell, triggering response. If these sender and receiver cells in direct physical proximity, signaling is labeled as paracrine. far signal transported, endocrine. this manuscript, others have argued past, I also argue that gasotransmitters must not be excluded endocrine molecules. that, like LEGO bricks built, dismantled, rebuilt into anything of builder's imagination,...

Studies in zebrafish can unravel the functions of cellular communication and thus identify novel bench-to-bedside drugs targeting signaling molecules. Due to incomplete annotation proteome, knowledge receptors, ligands, tools explore their interactome is limited. To address this gap, we

Presynaptic cGMP-gated ion (CNG) channels positively or negatively modulate neurotransmitter secretion as well the strength of synaptic transmission. Zebrafish channel, CNGA2a (a.k.a. CNGA5), was previously reported to be specifically enriched in terminals zebrafish oxytocin (OXT) neurons. This conclusion based on immunoreactivity a monoclonal antibody (mAb) clone L55/54, which directed against carboxy terminal tail CNGA2a. To study role neurons function, we generated mutants cnga2a, cnga2b...

In biochemistry textbooks, amino acids that are derived from environment and cannot be synthesized by cells classified as essential acids. However, the gasotransmitter field is currently not considering oxygen (O2) a gasotransmitter. This due to one of current criteria for gasotransmitter, which states 'It endogenously enzymatically generated, its production regulated’. Such restrictive has been applied For me, this equivalent at all. And such limitation will hinder challenge, proof,...

In biochemistry textbooks, amino acids that are derived from the diet classified as essential acids. However, metals such zinc described trace elements. Even though gaseous molecules termed gasotransmitters, term 'metallotransmitter' for is found in only one current peer-reviewed publication. Describing elements instead of metallotransmitters may impede investigation, validation, or refutation gasocrine signaling theory I recently proposed. Likewise, any organisms whose normal functioning...

To investigate gasocrine signaling, there is a critical need to identify gasoreceptors for the essential gasotransmitters like oxygen. Based on existing scientific literature, I propose that heme-based oxygen sensors, featuring diverse signaling domains across genera, should be explicitly designated as gasoreceptors. Acknowledging are likely belong multiple protein classes with and pathways will facilitate comprehensive search in all organisms every cell type. This approach broaden...