A5006511652- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
- Plant Pathogens and Fungal Diseases
- Potato Plant Research
- Plant biochemistry and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Cancer Treatment and Pharmacology
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Plant Gene Expression Analysis
- Constructed Wetlands for Wastewater Treatment
- Coleoptera Taxonomy and Distribution
- Plant Pathogens and Resistance
- Coastal wetland ecosystem dynamics
- Phytochemical Studies and Bioactivities
- Ginseng Biological Effects and Applications
- Plant Reproductive Biology
- Peatlands and Wetlands Ecology
- Forest Insect Ecology and Management
- Cassava research and cyanide
- Plant tissue culture and regeneration
- Physiological and biochemical adaptations
- Fish Ecology and Management Studies
- Botanical Research and Applications
- Postharvest Quality and Shelf Life Management
Weizmann Institute of Science
2013-2024
Melodea (Israel)
2024
Fraunhofer Institute for Molecular Biology and Applied Ecology
2010-2020
From Nasty to Tasty Some of our favorite food crops derive from wild relatives that were distasteful or even toxic. Domestication over many years selected for variants with reduced levels antinutritional compounds. The remain valuable, however, other traits such as resistance pathogens, but their use in crop development is complicated by the continued presence unpalatable Itkin et al. (p. 175 , published online 20 June) elucidate metabolic pathways and genes directing synthesis some these...
Taxol (paclitaxel) is a highly-oxygenated diterpenoid natural product first isolated from the pacific yew tree (Taxus brevifolia). It one of most widely used anticancer drugs. Soon after discovery its unique mode action and resulting high demand, an extensive search was initiated for alternative sources to replace slow-growing scarce yew. Thus far, however, related compounds have only been found in genus Taxus, which comprises small number plants with broad but generally geographical...
The aquatic Lemnaceae family, commonly called duckweed, comprises some of the smallest and fastest growing angiosperms known on Earth. Their tiny size, rapid growth by clonal propagation, facile uptake labeled compounds from media were attractive features that made them a well-known model for plant biology 1950 to 1990. Interest in duckweed has steadily regained momentum over past decade, driven part need identify alternative plants traditional agricultural crops can help tackle urgent...
Annotation of metabolites is an essential, yet problematic, aspect mass spectrometry (MS)-based metabolomics assays. The current repertoire definitive annotations metabolite spectra in public MS databases limited and suffers from lack chemical taxonomic diversity. Furthermore, the heterogeneity data prevents development universally applicable annotation tools. Here we present a combined experimental computational platform to advance this key issue metabolomics. WEIZMASS unique reference...
The genus Solanum comprises three food crops (potato, tomato, and eggplant), which are consumed on daily basis worldwide also producers of notorious anti-nutritional steroidal glycoalkaloids (SGAs). Hydroxylated SGAs (i.e. leptinines) serve as precursors for leptines that act defenses against Colorado Potato Beetle (Leptinotarsa decemlineata Say), an important pest potato worldwide. However, SGA hydroxylating enzymes remain unknown. Here, we discover 2-OXOGLUTARATE-DEPENDENT-DIOXYGENASE...
Summary Current innovations in mass‐spectrometry‐based technologies allow deep coverage of protein expression. Despite its immense value and contrast to transcriptomics, only a handful studies crop plants engaged with global proteome assays. Here, we present large‐scale shotgun proteomics profiling tomato fruit across two key tissues five developmental stages. A total 7738 individual groups were identified reliably measured at least one the analyzed or The depth our assay enabled...
Significance Plants synthesize a vast repertoire of steroidal specialized metabolites. These include the well-known class antinutritional glycoalkaloids (SGAs), which act as defensive chemicals in Solanaceae, and pharmacologically important widespread saponins. Here, we uncover an elusive enzymatic step that acts on unsaturated We find GLYCOALKALOID METABOLISM25 (GAME25) at key branch point biosynthesis pathways The activity GAME25 not only affects enormous diversity SGAs saponins, are...
Melleolides and related fungal sesquiterpenoid aryl esters are antimicrobial cytotoxic natural products derived from cultures of the Homobasidiomycetes genus <i>Armillaria</i>. The initial step in biosynthesis all melleolides involves cyclization universal sesquiterpene precursor farnesyl diphosphate to produce protoilludene, a reaction catalyzed by protoilludene synthase. We achieved partial purification synthase mycelial culture <i>Armillaria gallica</i> found that 6-protoilludene was its...
Organisms evolve mechanisms that regulate the properties of biogenic crystals to support a wide range functions, from vision and camouflage communication thermal regulation. Yet, mechanism underlying formation diverse intracellular remains enigmatic. Here we unravel biochemical control over crystal morphogenesis in zebrafish iridophores. We show chemical composition determines their shape, particularly through ratio between nucleobases guanine hypoxanthine. reveal these variations are...
Polyphenols are secondary metabolites produced by plants to defend themselves from environmental stressors. We explored the effect of
Regardless of major advances in mass spectrometry imaging (MSI), there are three intrinsic limitations associated with MSI, including intricate molecular identification, low coverage, and incapability to obtain "true" spatial distribution due isobaric particularly isomeric ions interference. We developed a novel approach that integrates vivo dual isotope labeling precursor metabolites MSI (DLEMMA-MS-Imaging) for identification spatially localized metabolite metabolic network map...
Taxol, a diterpenoid natural product first isolated from Taxus brevifolia, is one of today’s better known anticancer drugs. Despite its clinical efficacy, the difficulty establishing secure and cost-effective supply taxol has limited use. However, unique mode action efficacy against multiple forms cancer ensured continual efforts to achieve total semisynthesis, as well biotechnological production methods. Total synthesis now possible but inefficient, so related taxoids remains completely...
Suberized and/or lignified (i.e. lignosuberized) periderm tissue appears often on surface of fleshy fruit skin by mechanical damage caused following environmental cues or developmental programs. The mechanisms underlying lignosuberization remain largely unknown to date. Here, we combined an assortment microscopical techniques with integrative multi-omics approach comprising proteomics, metabolomics and lipidomics identify novel molecular components involved in lignosuberization. We chose...
In-source fragmentation (ISF) is a naturally occurring phenomenon in various ion sources including soft ionization techniques such as matrix-assisted laser desorption/ionization (MALDI). It has traditionally been minimized it makes the dataset more complex and often leads to mis-annotation of metabolites. Here, we introduce an approach termed PICA (for pixel intensity correlation analysis) that takes advantage ISF MALDI imaging increase confidence metabolite identification. In PICA,...
Abstract Melleolides and armillyl orsellinates are protoilludene-type aryl esters that synthesized exclusively by parasitic fungi of the globally distributed genus Armillaria (Agaricomycetes, Physalacriaceae). Several these compounds show potent antimicrobial cytotoxic activities, making them promising leads for development new antibiotics or drugs treatment cancer. We recently cloned characterized gallica gene Pro1 encoding protoilludene synthase, a sesquiterpene cyclase catalyzing...
Abstract Significant inter‐ and intraspecific genetic variation exists in duckweed, thus the potential for genome plasticity manipulation is high. Polyploidy recognised as a major mechanism of adaptation speciation plants. We produced several genome‐duplicated lines Landoltia punctata (Spirodela oligorrhiza) from both whole plants regenerating explants using colchicine‐based cocktail. These stably maintained an enlarged frond root morphology. DNA ploidy levels determined by...
Abstract Given the rapid growth and improvement in both mass spectrometry imaging (MSI) techniques its applications, there is a critical need for development of efficient comprehensive computational tools MSI data analysis. As such, we introduce ShinyCardinal, an open-source vendor-neutral software that covers all step It leverages R package Cardinal to enhance functionalities by introducing several additional important features, such as removal background noises matrix peaks, deisotoping,...