A5014421267- Epigenetics and DNA Methylation
- Prostate Cancer Treatment and Research
- Molecular Biology Techniques and Applications
- Genetic factors in colorectal cancer
- Cancer, Lipids, and Metabolism
- Cancer-related Molecular Pathways
- Cancer Genomics and Diagnostics
- Renal and related cancers
- RNA modifications and cancer
- Renal cell carcinoma treatment
- Cancer-related gene regulation
- Cancer Research and Treatments
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
- Ubiquitin and proteasome pathways
- Immunotherapy and Immune Responses
- Colorectal Cancer Treatments and Studies
- Cancer Cells and Metastasis
- FOXO transcription factor regulation
- Viral Infectious Diseases and Gene Expression in Insects
- Gene expression and cancer classification
- SARS-CoV-2 and COVID-19 Research
- Neuropeptides and Animal Physiology
- Lipid Membrane Structure and Behavior
- Genomics and Chromatin Dynamics
Institute of Molecular Biology and Genetics
2015-2024
National Academy of Sciences of Ukraine
2010-2024
Uppsala University
2016-2018
Institute for Scintillation Materials
2007
CYP-dependent metabolites play a critical role in regulating the cell cycle, as well proliferative, invasive, and migratory activity of cancer cells. We conducted study to analyze relative gene expression various
The SEMA3B gene is located in the 3p21.3 LUCA region, which frequently affected different types of cancer. objective our study was to expand knowledge as a tumor suppressor and mechanisms its inactivation. In this study, several experimental approaches were used: growth analyses apoptosis assays vitro SCID mice, expression methylation other. With use small cell lung cancer line U2020 we confirmed function suppressor, showed that suppression can be realized through induction and, possibly,...
A significant need for reliable and accurate cancer diagnostics prognosis compels the search novel biomarkers that would be able to discriminate between indolent aggressive tumors at early stages of disease. The aim this work was identification potential diagnostic characterization different types prostate tumors. NotI-microarrays with 180 clones associated chromosome 3 genes/loci were applied determine genetic epigenetic alterations in 33 For 88 clones, aberrations detected more than 10%...
of our study was to identify and characterize the SARS-CoV-2 variants in COVID-19 patients' samples collected from different regions Ukraine determine relationship between phylogenetics epidemiology.
Chromosome 3-specific NotI microarray (NMA) containing 180 clones with 188 genes was used in the study to analyze 18 high grade serous ovarian cancer (HGSOC) samples and 7 benign tumors. We aimed find novel methylation-dependent biomarkers for early detection prognosis of HGSOC. Thirty five markers showed frequency methylation/deletion more or equal 17%. To check results NMA hybridizations several four (LRRC3B, THRB, ITGA9 RBSP3 (CTDSPL)) were bisulfite sequenced confirmed hybridization. A...
Background: Prostate cancer is one of the most common male cancers in Western countries and takes third place morbidity Ukraine. It a highly heterogeneous disease. Aim: To analyze relative expression levels TGFB1, IL1B, FOS, EFNA5, TAGLN, PLAU, EPDR1 genes malignant non-malignant prostate tissues. Materials Methods: Total RNA was isolated from 16 adenomas, 37 adenocarcinomas, 29 conventionally normal gene quantitative real-time polymerase chain reaction performed. Results: The significant...
Aim: To find putative diagnostic markers for clear cell renal carcinomas (ccRCC). Material and methods: Quantitative polymerase chain reaction (Q-PCR), bisulfite treatment, methylation-specific PCR, analysis on cBioPortal Cancer Genomics. Results: We have found that expression of GPX 1, GPX3, GPX4 genes was decreased in ccRCC. shown the number alanine (GCG) repeats at amino terminus GPX1 protein is variable. It reported earlier an allele possess 5 associated with increased cancer risk....
To assess relative expression (RE) levels of CAF-, TAM-specific, immune defense-associated genes in prostate tumors and to show correlation RE with clinical, pathological molecular characteristics, the aim define clinically significant specific alterations a gene pattern.RE 23 was analyzed by quantitative polymerase chain reaction 37 freshly frozen samples cancer tissues different Gleason score (GS) at various tumor stages, compared paired conventionally normal tissue (CNT) 20...
To analyze an expression pattern of the steroid and peptide hormone receptors, metabolic enzymes EMT-related genes in prostate tumors relation to presence TMPRSS2/ERG fusion; examine a putative correlation between gene clinical characteristics, define molecular subtypes cancer.The relative (RE) 33 transcripts (27 genes) presence/absence fusion were analyzed by quantitative PCR. 37 cancer tissues (T) paired with conventionally normal tissue (CNT) 21 samples adenomas investigated. RE changes...
Aim.To detect expression of EMT-related genes in prostate tumor samples and analyze a possible correlation between the gene level clinical characteristics cancer different groups.Methods.Expression 19 was analyzed 37 frozen tissues at stages Gleason scores, paired conventionally normal 20 adenomas, using quantitative PCR.Results.We have found that nine were expressed differently benign malignant tumors, namely AR (isoform 1), 2), PTEN, VIM, MMP9, KRT18, PCA3, HOTAIR SCHLAP1.When compared, we...
Keywords: renal cell carcinoma, genetic and epigenetic regulation, chromosome 3, quantitative real time PCR, methylation status
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Aim.To detect ETS fusion transcripts in Ukrainian population and to analyze a possible relationship between the clinical characteristics of prostate cancer.Methods.Quantitative PCR (q-PCR) was used expression six at mRNA level.The amplified products were analyzed by gel electrophoresis direct sequencing.We 37 fresh frozen samples cancer tissues, paired conventionally normal tissue 20 adenomas.Results.Six TMPRSS2 with ERG, ETV1, ETV4, ETV5 analyzed.Only one out detected cohort patients...
Keywords: epithelial tumor, epigenetic markers, NotI-microarrays, early cancer detection, prognosis of cancer, tumor-suppressor gene
Prostate cancer is one of the main causes mortality in men with malignant tumors. The urgent problem was a search for biomarkers prostate cancer, which would allow distinguishing between aggressive metastatic and latent aim this work to differentially expressed genes normal epithelial cells PNT2 cell lines LNCaP, DU145 PC3, produced from tumors different aggressiveness ability. Such might be used create panel prognostic markers metastasis. Relative gene expression 65 cancer-related...
Keywords: renal cell carcinoma, genetic and epigenetic regulation, quantitative real time PCR, deletion, methylation status
Ïîøóê ãåí³â -ïîòåíö³éíèõ ìàðêåð³â àãðåñèâíîñò³ ³ ìåòàñòàçóâàííÿ ðàêó ïðîñòàòè ëþäèíè ª.Å. Ðîçåíáåðã 1 , Ò. Þ. Ïðóäíèêîâà 2 Ã. Â. Ãåðàùåíêî Å. Ãðèãîðü'ºâà ²
Keywords: carcinogenesis, oncogenes, tumor suppressor genes, deletions, amplification, LOH, somatic and germline mutations, promoter methylation, noncoding RNA, NGS
The study aimed to identify the clinically relevant gene variants in colon adenocarcinoma samples of Ukrainian patients using NGS Comprehensive Cancer Panel (CCP) implement them conveniently clinical practice. Methods. We have studied 20 with colorectal adenocarcinomas various differentiation grades. To variants, CCP data were filtered Franklin by Genoox database. Results. A total 79 variant alterations (SNVs, INDELs) found 28 409 genes. largest number mutations was 3 genes, APC, TP53, and...