A5051461846- Nerve injury and regeneration
- Pluripotent Stem Cells Research
- Neurogenesis and neuroplasticity mechanisms
- Autism Spectrum Disorder Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Axon Guidance and Neuronal Signaling
- Neuroscience and Neuropharmacology Research
- Macrophage Migration Inhibitory Factor
- Nuclear Receptors and Signaling
- 3D Printing in Biomedical Research
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Microfluidic and Bio-sensing Technologies
- Adenosine and Purinergic Signaling
- Cancer Treatment and Pharmacology
- Growth Hormone and Insulin-like Growth Factors
- Glioma Diagnosis and Treatment
- Multiple Sclerosis Research Studies
- Spinal Cord Injury Research
- Neuroblastoma Research and Treatments
- Prenatal Substance Exposure Effects
- Electrohydrodynamics and Fluid Dynamics
- Neuropeptides and Animal Physiology
- Hormonal Regulation and Hypertension
- Chemokine receptors and signaling
University of Southern Denmark
2015-2024
St. Joseph’s Healthcare Hamilton
2022
McMaster University
2022
Odense University Hospital
2017-2019
Region of Southern Denmark
2017
Institute of Molecular Medicine
2016
Uppsala University
2005-2014
Stockholm University
2009
Icahn School of Medicine at Mount Sinai
2002-2005
University of Cambridge
2005
Nodes of Ranvier are regularly placed, nonmyelinated axon segments along myelinated nerves. Here we show that nodal membranes isolated from the central nervous system (CNS) mammals restricted neurite outgrowth cultured neurons. Proteomic analysis these revealed several inhibitors outgrowth, including oligodendrocyte myelin glycoprotein (OMgp). In rat spinal cord, OMgp was not localized to compact myelin, as previously thought, but oligodendroglia-like cells, whose processes converge form a...
A streamlined, simple technique for primary cell culture from E17 rat tissue is presented. In an attempt to standardize culturing methods all neuronal types in the embryo, we evaluated a commercial medium without serum and used similar times trypsinization tested different surfaces plating. 1 day, using one method single medium, it possible produce robust cultures of dorsal root ganglia (DRG), cerebellum, enteric plexi. Allowing endogenous glial cells repopulate saves time compared with...
Background: The cuprizone (CPZ) model of multiple sclerosis (MS) was used to identify miRNAs related in vivo de- and remyelination. We further investigated the role miR-146a miR-146a-deficient (KO) mice: this miRNA is differentially expressed MS lesions promotes differentiation oligodendrocyte precursor cells (OPCs) during remyelination, but its has not been examined demyelination. Methods: MicroRNAs were by Agilent Mouse Microarray corpus callosum CPZ-induced demyelination Demyelination,...
Objective Here, we applied a multi-omics approach (i) to examine molecular pathways related de- and remyelination in multiple sclerosis (MS) lesions; (ii) translate these findings the CSF proteome order identify molecules that are differentially expressed among MS subtypes. Methods To relate genes lesions remyelination, compared transcriptome of cuprizone (CPZ)-induced remyelination. Protein products overlapping orthologous were measured within by quantitative proteomics, parallel reaction...
Paclitaxel‐induced peripheral neuropathy (PIPN) is a common and dose‐limiting adverse event. The role of P‐glycoprotein (P‐gp) in the neuronal efflux paclitaxel was assessed using translational approach. SH‐SY5Y cells were differentiated to neurons toxicity absence presence P‐gp inhibitor determined. Paclitaxel caused marked dose‐dependent SH‐SY5Y‐derived neurons. neurotoxicity exacerbated with concomitant inhibition by valspodar verapamil, consistent increased intracellular accumulation...
Background: MiR-146a is an important regulator of innate inflammatory responses and also implicated in cell death survival. Methods: By sorting CNS resident cells, microglia were the main cellular source miR-146a. Therefore, we investigated function phenotype miR-146a knock-out (KO) mice, analyzed proteome KO wild-type (WT) by LC-MS/MS, examined expression different brain lesions patients with multiple sclerosis (MS). Results: When stimulated LPS or myelin vitro, from mice expressed higher...
Several motility disorders originate in the enteric nervous system (ENS). Our knowledge of factors governing survival ENS is poor. Changes expression vasoactive intestinal peptide (VIP) and nitric oxide synthase (NOS) neurons occur after neuronal injury adaptation. The aim this study was to evaluate whether VIP (NO) influence cultured, dissociated myenteric neurons. Neuronal evaluated 0, 4, 8 days culture. Influence NO on examined culturing presence VIP, donor, antiserum, or NOS inhibitor. A...
The effects of estrogen and progesterone on Schwann cell proliferation were studied in cultured segments the rat sciatic nerve from adult male, female, newborn rats, by measurement [3H thymidine incorporation or bromo-deoxy-uridine- (BrdU)-labelling immunocytochemistry. Estrogen (100 nM–500 nM) enhanced [3H] male while it had no effect female rats. Progesterone stimulated rats nM), but caused only a small proliferative response cells at high concentrations. blocked when presence inhibitors...
N-cadherin comprises five homologous extracellular domains, a transmembrane, and cytoplasmic domain. The domains of play important roles in homophilic cell adhesion, but the contribution each domain to this phenomenon has not been fully evaluated. In particular, following questions remain unanswered: what is minimal combination that can generate how organization related adhesive strength, does serve facilitate interaction? To address these issues, we made serial constructs produced various...
The evolutionary origin of myelinating cells in the vertebrate nervous system remains a mystery. A clear delineation developmental potentialities neuronal support CNS and PNS might aid formulating hypothesis about origins cells. Although glial-precursor cell can differentiate into oligodendrocytes (OLs), Schwann (SCs) astrocytes, homologous multipotential has not yet been found PNS. Here, we identify type embryonic dorsal root ganglia (DRG) – satellite that develops OLs, SCs astrocytes....
and Purpose: Chemotherapy-induced peripheral neuropathy (CIPN) constitutes a significant health problem due to the increasing prevalence lack of therapies for treatment prevention. While pivotal routine cancer treatment, paclitaxel vincristine frequently cause CIPN impact quality life among patients survivors. Here, we investigate molecular mechanisms drug transport in CIPN.
The factors that control proliferation of Schwann cells during peripheral nerve regeneration are not yet known. In this study we investigated the effects insulin, insulin-like growth factor I and II (IGF-I IGF-II), IGF-I analogues, interfere with their respective receptors, on [3H]thymidine incorporation into cultured segments from rat sciatic nerve. Segments in nM (0.1-1.7 nM) concentrations truncated (tIGF-I), long R3IGF-I, or IGF-II exhibited an increase compared segments. was most...
Abstract Lipoprotein receptor‐related protein‐2 (LRP2)/megalin is a member of the low density lipoprotein receptor (LDLR) family, and essential in absorptive epithelia for endocytosis lipoproteins, molecular weight proteins, cholesterol vitamins, as well cellular signaling. Previous studies have shown megalin expression ependymal cells choroid plexus. We investigated spinal cord postnatal mice with immunohistochemistry immunoblot. Antibodies recognizing either cytoplasmic tail (MM6) or...
Macrophage migration inhibitory factor (MIF), a small conserved protein, is abundant in the immune- and central nervous system (CNS). MIF has several receptors binding partners that can modulate its action on cellular level. It upregulated neurodegenerative diseases cancer although function far from clear. Here, we report finding of new partner to MIF, serine protease HTRA1. This enzyme cleaves growth factors, extracellular matrix molecules implicated some same as MIF. We show between HTRA1...
Cladribine (CdA), an oral prodrug approved for the treatment of relapsing multiple sclerosis, selectively depletes lymphocytes. CdA passes blood-brain barrier, suggesting a potential effect on central nervous system (CNS) resident cells. We examined if modifies phenotype and function naive activated primary mouse microglia, when applied in concentrations 0·1-1 μM that putatively overlap human cerebrospinal fluid (CSF) concentrations. Primary microglia cultures without stimulation or presence...
Polybrominated diphenyl ethers (PBDEs) are commonly used flame retardants in various consumer products. Pre- and postnatal exposure to congeners of PBDEs disrupts normal brain development rodents. Two-dimensional difference gel electrophoresis (2D-DIGE) was analyze concentration-dependent differences protein expression cultured cortical cells isolated from rat fetuses (GD 21) after 24 h PBDE-99 (3, 10, or 30 microM). Changes on a post-translational level were studied using 1 microM PBDE-99....
Journal Article The modular xylanase Xyn10A from Rhodothermus marinus is cell-attached, and its C-terminal domain has several putative homologues among cell-attached proteins within the phylum Bacteroidetes Get access Eva Nordberg Karlsson, Karlsson Department Biotechnology, Center for Chemistry Chemical engineering, Lund University, P.O.Box 124, SE-221 00 Lund, Sweden *Corresponding author. Tel.: +46 46 222 26; fax: 4713, E-mail address: [email protected] Search other works by this author...