A5009266215- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Probiotics and Fermented Foods
- Phytase and its Applications
- Food composition and properties
- Glycosylation and Glycoproteins Research
- Infant Nutrition and Health
- Enzyme Catalysis and Immobilization
- Digestive system and related health
- Carbohydrate Chemistry and Synthesis
- Polysaccharides and Plant Cell Walls
- Enzyme Structure and Function
- Gut microbiota and health
- Microbial Metabolic Engineering and Bioproduction
- Peptidase Inhibition and Analysis
- Proteins in Food Systems
- Protein Hydrolysis and Bioactive Peptides
- Bacteriophages and microbial interactions
- Polysaccharides Composition and Applications
- Cassava research and cyanide
- Enzyme-mediated dye degradation
- Studies on Chitinases and Chitosanases
- Protease and Inhibitor Mechanisms
- Pediatric Hepatobiliary Diseases and Treatments
Technical University of Denmark
2016-2025
Digital Proteomics (United States)
2016
Carlsberg Laboratory
2005-2012
Columbia University
2011
Institut de Biologie et de Chimie des Protéines
2005
Lund University
1999-2004
Newcastle University
2002
University of Sheffield
2002
Abstract β-Mannans are plant cell wall polysaccharides that commonly found in human diets. However, a mechanistic understanding into the key populations degrade this glycan is absent, especially for dominant Firmicutes phylum. Here, we show prominent butyrate-producing Firmicute Roseburia intestinalis expresses two loci conferring metabolism of β-mannans. We combine multi-“omic” analyses and detailed biochemical studies to comprehensively characterize loci-encoded proteins involved β-mannan...
The human gut microbiota established during infancy has persistent effects on health. In vitro studies have suggested that milk oligosaccharides (HMOs) in breast promote the formation of a bifidobacteria-rich infant guts; however, underlying molecular mechanism remains elusive. Here, we characterized two functionally distinct but overlapping fucosyllactose transporters (FL transporter-1 and -2) from Bifidobacterium longum subspecies infantis. Fecal DNA HMO consumption analyses, combined with...
The mucolytic human gut microbiota specialist Akkermansia muciniphila is proposed to boost mucin-secretion by the host, thereby being a key player in mucus turnover. Mucin glycan utilization requires removal of protective caps, notably fucose and sialic acid, but enzymatic details this process remain largely unknown. Here, we describe specificities ten A. glycoside hydrolases, which collectively remove all known sialyl fucosyl mucin caps including those on double-sulfated epitopes....
Abstract The early life human gut microbiota exerts life-long health effects on the host, but mechanisms underpinning its assembly remain elusive. Particularly, colonization of Clostridiales from Roseburia - Eubacterium group, associated with protection colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support growth members distinct milk oligosaccharides (HMOs). HMO pathways, which include enzymes a previously unknown structural fold...
Therapeutically active glycosylated phytochemicals are ubiquitous in the human diet. The gut microbiota (HGM) modulates bioactivities of these compounds, which consequently affect host physiology and composition. Despite a significant impact on health, key players underpinning mechanisms this interplay remain uncharacterized. Here, we demonstrate growth Lactobacillus acidophilus mono- diglucosyl dietary plant glycosides (PGs) possessing small aromatic aglycones. Transcriptional analysis...
Oxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative remains limited. Here, a phylogenetic analysis auxiliary activity 7 family (AA7), currently harbouring oligosaccharide flavo-oxidases, reveals striking abundance AA7-genes phytopathogenic fungi Oomycetes. Expression five fungal enzymes, including three from unexplored clades, expands AA7-substrate range unveils...
α-Glucanotransferases of the CAZy family GH70 convert starch-derived donors to industrially important α-glucans. Here, we describe characteristics a novel GtfB-type 4,6-α-glucanotransferase high enzyme activity (60.8 U mg
Probiotic microbes rely on their ability to survive in the gastrointestinal tract, adhere mucosal surfaces, and metabolize available energy sources from dietary compounds, including prebiotics. Genome sequencing projects have proposed models for understanding prebiotic catabolism, but mechanisms remain be elucidated many substrates. Although β-galactooligosaccharides (GOS) are documented little is known about utilization by lactobacilli. This study aimed identify genetic loci Lactobacillus...
α-Amylases are glucan hydrolases that cleave α-1,4-glucosidic bonds in starch. In vascular plants, α-amylases can be classified into three subfamilies. Arabidopsis has one member of each subfamily. Among them, only AtAMY3 is localized the chloroplast. We expressed and purified from Escherichia coli carried out a biochemical characterization protein to find factors regulate its activity. Recombinant was active toward both insoluble starch granules soluble substrates, with strong preference...
The human gastrointestinal tract can be positively modulated by dietary supplementation of probiotic bacteria in combination with prebiotic carbohydrates. Here differential transcriptomics and functional genomics were used to identify genes Lactobacillus acidophilus NCFM involved the uptake catabolism 11 potential compounds consisting α- β- linked galactosides glucosides. These oligosaccharides induced encoding phosphoenolpyruvate-dependent sugar phosphotransferase systems (PTS), galactoside...
Abstract Background Probiotic bifidobacteria in combination with prebiotic carbohydrates have documented positive effects on human health regarding gastrointestinal disorders and improved immunity, however the selective routes of uptake remain unknown for most candidate prebiotics. The differential transcriptomes Bifidobacterium animalis subsp. lactis Bl-04, induced by 11 potential oligosaccharides were analyzed to identify genetic loci involved catabolism α- β-linked hexoses, β-xylosides....
Some polysaccharide processing enzymes possess secondary carbohydrate binding sites situated on the surface far from active site. In barley α-amylase 1 (AMY1), two such sites, SBS1 and SBS2, are found catalytic (β/α)8-barrel noncatalytic C-terminal domain, respectively. Site-directed mutagenesis of Trp278 Trp279, stacking onto adjacent ligand glucosyl residues at SBS1, Tyr380 His395, making numerous contacts suggested that SBS2 act synergistically in degradation starch granules. While makes...
Summary Glycan utilization plays a key role in modulating the composition of gut microbiota, but molecular insight into oligosaccharide uptake by this microbial community is lacking. Arabinoxylo‐oligosaccharides ( AXOS ) are abundant diet, and selectively fermented probiotic bifidobacteria colon. Here we show how selectivity for established strain B ifidobacterium animalis subsp. lactis Bl ‐04. The binding protein AXBP , which associated with an ATP ‐binding cassette ABC transporter that...
Isomaltooligosaccharides (IMO) have been suggested as promising prebiotics that stimulate the growth of probiotic bacteria. Genomes lactobacilli from acidophilus group, represented by Lactobacillus NCFM, encode α-1,6 glucosidases family GH13_31 (glycoside hydrolase 13 subfamily 31) confer degradation IMO. These genes reside frequently within maltooligosaccharide utilization operons, which include an ATP-binding cassette transporter and α-glucan active enzymes, e.g., maltogenic amylases...
Surface binding sites (SBSs) interact with carbohydrates outside of the enzyme active site. They are frequently situated on catalytic domains and distinct from carbohydrate modules (CBMs). SBSs found in a variety enzymes often seen crystal structures. Notably about half > 45 (in 17 GH two GT families) an identified SBS GH13 few GH77, both belonging to clan GH-H enzymes. The many provide opportunity analyse their distribution within this very large diverse family. containing spread among 15...
Starch is the second most abundant plant-derived biomass and a major feedstock in non-food industrial applications first generation biofuel production. In contrast to lignocellulose, detailed insight into fungal degradation of starch currently lacking. This study explores secretomes Aspergillus nidulans grown on cereal starches from wheat high-amylose (HA) maize, as well legume pea for 5 days. grew efficiently starches, whereas growth was poor. The at days 3–5 were starch-type dependent also...
Recombinantly produced biotherapeutics hold promise for improving the current standard of care snakebite envenoming over conventional serotherapy. Nanobodies have performed well in clinic, and context antivenom, they shown ability to neutralize long α-neurotoxins