A5049145534- Probiotics and Fermented Foods
- Infant Nutrition and Health
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Digestive system and related health
- Enzyme Production and Characterization
- Glycosylation and Glycoproteins Research
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Hemoglobin structure and function
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Drug Transport and Resistance Mechanisms
- Bacterial Genetics and Biotechnology
- Microscopic Colitis
- Enterobacteriaceae and Cronobacter Research
- Blood groups and transfusion
- Viral gastroenteritis research and epidemiology
Technical University of Denmark
2019-2024
The mucolytic human gut microbiota specialist Akkermansia muciniphila is proposed to boost mucin-secretion by the host, thereby being a key player in mucus turnover. Mucin glycan utilization requires removal of protective caps, notably fucose and sialic acid, but enzymatic details this process remain largely unknown. Here, we describe specificities ten A. glycoside hydrolases, which collectively remove all known sialyl fucosyl mucin caps including those on double-sulfated epitopes....
Abstract The early life human gut microbiota exerts life-long health effects on the host, but mechanisms underpinning its assembly remain elusive. Particularly, colonization of Clostridiales from Roseburia - Eubacterium group, associated with protection colorectal cancer, immune- and metabolic disorders is enigmatic. Here, we describe catabolic pathways that support growth members distinct milk oligosaccharides (HMOs). HMO pathways, which include enzymes a previously unknown structural fold...
Abstract Butyrate-producing human gut microbiota members are recognized for their strong association with a healthy immune-homeostasis and protection from inflammatory disorders colorectal cancer. These effects attributed to butyrate, the terminal electron sink of glycan fermentation by prevalent abundant colonic Firmicutes Lachnospiraceae Oscillospiraceae families. Remarkably, our insight into utilization mechanisms preferences butyrogenic remains very limited as compared other symbionts,...
Abstract Post-weaning diarrhoea (PWD) in piglets presents a widespread problem industrial pig production and is often caused by enterotoxigenic E. coli (ETEC) strains. Current solutions, such as antibiotics medicinal zinc oxide, are unsustainable increasingly being prohibited, resulting dire need for novel solutions. Thus, this study, we propose evaluate protein-based feed additive, comprising two bivalent heavy chain variable domain (V H H) constructs H-(GGGGS) 3 -V H, BL1.2 BL2.2) an...
A major challenge in industrial pig production is the prevalence of post-weaning diarrhea (PWD) piglets, often caused by enterotoxigenic Escherichia coli (ETEC). The increased use antibiotics and zinc oxide to treat PWD has raised global concerns regarding antimicrobial resistance development environmental pollution. Still, alternative treatments targeting ETEC counteracting are largely lacking. Here, we report design a pH, temperature, protease-stable bivalent VHH-based protein BL1.2 that...
Summary Human gut bifidobacteria rely on ATP‐binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide‐specific solute‐binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize Bl MnBP1 and MnBP2, two SBPs to β‐manno‐oligosaccharide (MnOS) transporter in Bifidobacterium animalis subsp. lactis . Despite similar overall specificity preference mannotriose...
The degradation of starch in the small intestine generates short linear and branched α-glucans. latter are poorly digestible by humans, rendering them available to gut microbiota, e.g., lactobacilli adapted considered beneficial health. This study unveils a previously unknown scheme maltooligosaccharide (MOS) catabolism via concerted activity an 1,4-α-glucosyltransferase together with classical hydrolase phosphorylase. intriguing involvement glucosyltransferase likely allows fine-tuning...
Abstract The gut mucolytic specialist Akkermansia muciniphila is strongly associated with the integrity of mucus layer. Mucin glycan utilization requires removal diverse protective caps, notably, fucose and sialic acid, but enzymatic details this process remain largely unknown. Here, we describe specificities ten A. glycoside hydrolases, which collectively remove all known sialyl fucosyl mucin caps including those double sulphated epitopes. Structural analyses revealed an unprecedented...
ABSTRACT The maltooligosaccharide (MOS) utilization locus in Lactobacillus acidophilus NCFM, a model for human small-intestine lactobacilli, encodes family 13 subfamily 31 glycoside hydrolase (GH13_31), annotated as an 1,6-α-glucosidase. Here, we reveal that this enzyme ( La GH13_31B) is 1,4-α-glucosyltransferase disproportionates MOS with preference maltotriose. GH13_31B acts concert maltogenic α-amylase efficiently releases maltose from larger than Collectively, these two enzymes promote...
Abstract The early life human gut microbiota exerts life-long health effects on the host, but mechanisms underpinning its assembly remain elusive. Particularly, colonization of Clostridiales from Roseburia-Eubacterium group, associated with protection colorectal cancer, immune- and metabolic disorders is enigmatic. Here we unveil growth Roseburia Eubacterium members milk oligosaccharides (HMOs) using an unprecedented catabolic apparatus. described HMO pathways additional glycan utilization...