A5076557273- Enzyme Production and Characterization
- Microbial Metabolites in Food Biotechnology
- Enzyme Structure and Function
- Biofuel production and bioconversion
- Phytase and its Applications
- Diet, Metabolism, and Disease
- Glycosylation and Glycoproteins Research
- Enzyme Catalysis and Immobilization
- Probiotics and Fermented Foods
- Digestive system and related health
- Food composition and properties
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Carbohydrate Chemistry and Synthesis
- Biochemical and Molecular Research
- Plant Stress Responses and Tolerance
- Algal biology and biofuel production
- Polyamine Metabolism and Applications
- Plant Parasitism and Resistance
- Plant Gene Expression Analysis
- Insect-Plant Interactions and Control
- Microbial metabolism and enzyme function
- GABA and Rice Research
- Peptidase Inhibition and Analysis
- Protease and Inhibitor Mechanisms
Hokkaido University
2015-2025
Nihon Nohyaku (Japan)
2008-2012
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved synthesis, modification degradation complex carbohydrates. is closely connected with actively curated CAZy database, which provides sequence-based foundation for biochemical, mechanistic structural characterization these diverse proteins. Now celebrating its 10th anniversary online, successful example dynamic,...
α-Glucosidases, which catalyze the hydrolysis of α-glucosidic linkage at nonreducing end substrate, are important for metabolism α-glucosides. Halomonas sp. H11 α-glucosidase (HaG), belonging to glycoside hydrolase family 13 (GH13), only has high hydrolytic activity towards α-(1 → 4)-linked disaccharide maltose among naturally occurring substrates. Although several three-dimensional structures GH13 members have been solved, specificity and 4) recognition mechanism unclear owing a lack...
Abstract Cellobiose 2-epimerase (CE) catalyzes C-2 epimerization of reducing end d-glucose/d-mannose residue β-(1→4)-disaccharides, and also slightly aldose-ketose conversion. In this study, we investigated the structure-function relationship Rhodothermus marinus CE (RmCEs). 2H2O, 2H replaced 2-H sugar residue, suggesting a proton abstraction-addition mechanism via cis-enediolate intermediate. The structure RmCE-mannobiitol complex showed that His259 was suitable for abstracting from...
Cellobiose 2-epimerase (CE) reversibly converts d-glucose residues into d-mannose at the reducing end of unmodified β1,4-linked oligosaccharides, including β-1,4-mannobiose, cellobiose, and lactose. CE is responsible for conversion β1,4-mannobiose to 4-O-β-d-mannosyl-d-glucose in mannan metabolism. However, detailed catalytic mechanism unclear due lack structural data complex with ligands. We determined crystal structures halothermophile Rhodothermus marinus (RmCE) substrates/products or...
Cellobiose 2-epimerase (CE) reversibly converts glucose residue to mannose at the reducing end of β-1,4-linked oligosaccharides. It efficiently produces epilactose carrying prebiotic properties from lactose, but utilization known CEs is limited due thermolability. We focused on thermoholophilic Rhodothermus marinus JCM9785 as a CE producer, since CE-like gene was found in genome R. DSM4252. activity detected cell extract JCM9785. The deduced amino acid sequence (RmCE) 94.2% identical that...
Cold shock proteins (CSPs) of bacteria are produced in response to cold and function as RNA chaperones that essential for adaptation. Arabidopsis thaliana COLD SHOCK DOMAIN PROTEIN 3 (AtCSP3) shares a domain with bacterial CSPs is involved acquisition freezing tolerance. Our previous study revealed many the genes down regulated an AtCSP3 knockout mutant ( atcsp3–2 ) functionally associated responses salt drought well cold. Here, we examined involvement stress We found induced during...
Enzymatic epimerization is an important modification for carbohydrates to acquire diverse functions attributable their stereoisomers. Cellobiose 2-epimerase (CE) catalyzes interconversion between d-glucose and d-mannose residues at the reducing end of β-1,4-linked oligosaccharides. Here, we solved structure Ruminococcus albus CE (RaCE). The RaCE showed strong similarity those N-acetyl-D-glucosamine aldose-ketose isomerase YihS with a high degree conservation around catalytic center, although...
An α-glucosidase (HaG) with the following unique properties was isolated from Halomonas sp. strain H11: (i) high transglucosylation activity, (ii) activation by monovalent cations, and (iii) very narrow substrate specificity. The molecular mass of purified HaG estimated to be 58 kDa sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). showed hydrolytic activities toward maltose, sucrose, p-nitrophenyl α-D-glucoside (pNPG) but almost no other disaccharides or...
Abstract Obesity is one of the major health problems throughout world. The present study investigated preventive effect epilactose – a rare non-digestible disaccharide on obesity and metabolic disorders in mice fed high-fat (HF) diets. Feeding with HF diets increased body weight gain, fat pad adipocyte size ( P <0·01), these increases were effectively prevented by use supplemental without influencing food intake <0·01). Caecal pools SCFA such as acetic propionic acids higher compared...
α-Glucosidase hydrolyzes α-glucosides and transfers α-glucosyl residues to an acceptor through transglucosylation. In this study, GH13_31 α-glucosidase BspAG13_31A with high transglucosylation activity is reported in Bacillus sp. AHU2216 biochemically structurally characterized. This enzyme specific α-(1→4)-glucosidic linkage as substrates products. Maltose the most preferred substrate. Crystal structures of wild-type for substrate-free form inactive acid/base mutant E256Q complexes...
Cellobiose 2-epimerase (CE), found mainly in anaerobes, reversibly converts d-glucose residues at the reducing end of β-1,4-linked oligosaccharides to d-mannose residues. In this study, we characterized CE-like proteins from various aerobes (Flavobacterium johnsoniae NBRC 14942, Pedobacter heparinus 12017, Dyadobacter fermentans ATCC 700827, Herpetosiphon aurantiacus 23779, Saccharophagus degradans 43961, Spirosoma linguale 33905, and Teredinibacter turnerae 39867), because aerobes, more...
Summary Human gut bifidobacteria rely on ATP‐binding cassette (ABC) transporters for oligosaccharide uptake. Multiple oligosaccharide‐specific solute‐binding protein (SBP) genes are occasionally associated with a single ABC transporter, but the significance of this multiplicity remains unclear. Here, we characterize Bl MnBP1 and MnBP2, two SBPs to β‐manno‐oligosaccharide (MnOS) transporter in Bifidobacterium animalis subsp. lactis . Despite similar overall specificity preference mannotriose...
Salicylic acid (SA) is a phytohormone that regulates variety of physiological and developmental processes, including disease resistance. SA key signaling component in the immune response many plant species. However, mechanism underlying SA-mediated immunity obscure rice (Oryza sativa). Prior analysis revealed correlation between basal level blast resistance range varieties. This suggested might be improved by increasing level. Here, we identified novel UDP-glucosyltransferase gene, UGT74J1,...
A practical purification method for a non-digestible disaccharide, epilactose (4-O-β-galactosyl-d-mannose), was established. Epilactose synthesized from lactose with cellobiose 2-epimerase and purified by the following procedure: (i) removal of crystallization, (ii) hydrolysis β-galactosidase, (iii) digestion monosaccharides yeast, (iv) column chromatography Na-form cation exchange resin. 91.1% purity recovered at 42.5% yield.
Cellobiose 2-epimerase (CE) efficiently forms epilactose which has several beneficial biological functions. A thermostable CE from Rhodothermus marinus was immobilized on Duolite A568 and packed into a column. Lactose (100 g/L) supplied to the reactor, kept at 50 °C space velocity of 8 h(-1). The concentration resulting eluate 30 g/L, this maintained for 13 d.
Ruminococcus albus has the ability to intracellularly degrade cello-oligosaccharides primarily via phosphorolysis. In this study, enzymatic characteristics of R. cellodextrin phosphorylase (RaCDP), which is a member glycoside hydrolase family 94, was investigated. RaCDP catalyzes phosphorolysis cellotriose through an ordered 'bi bi' mechanism in binds before inorganic phosphate, and then cellobiose glucose 1-phosphate (Glc1P) are released that order. Among tested, had highest phosphorolytic...
Dextran glucosidase from Streptococcus mutans (SmDG), which belongs to glycoside hydrolase family 13 (GH13), hydrolyzes the non-reducing terminal glucosidic linkage of isomaltooligosaccharides and dextran. Thermal deactivation SmDG did not follow single exponential decay but rather two-step irreversible model, involves an active intermediate having 39% specific activity. The presence a low concentration CaCl2 increased thermostability SmDG, mainly due marked reduction in rate constant...
In plants, cis-jasmone (CJ) is synthesized from α-linolenic acid (LA) via two biosynthetic pathways using jasmonic (JA) and iso-12-oxo-phytodienoic (iso-OPDA) as key intermediates. However, there have been no reports documenting CJ production by microorganisms. the present study, of fungal-derived Lasiodiplodia theobromae was observed for first time, although this not Botrytis cinerea, Verticillium longisporum, Fusarium oxysporum, Gibberella fujikuroi, Cochliobolus heterostrophus. To...