A5057278945- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Advanced Cellulose Research Studies
- Carbohydrate Chemistry and Synthesis
- Microbial Metabolites in Food Biotechnology
- Transgenic Plants and Applications
- biodegradable polymer synthesis and properties
- Catalysis for Biomass Conversion
- Studies on Chitinases and Chitosanases
- Calcium Carbonate Crystallization and Inhibition
- Food composition and properties
- Plant-Microbe Interactions and Immunity
- Biotin and Related Studies
- Vibrio bacteria research studies
- Legume Nitrogen Fixing Symbiosis
- thermodynamics and calorimetric analyses
- Insect and Arachnid Ecology and Behavior
- Bioeconomy and Sustainability Development
- Hydrogels: synthesis, properties, applications
- Protein purification and stability
- Electrospun Nanofibers in Biomedical Applications
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2024
Centre National de la Recherche Scientifique
2017-2024
Université de Toulouse
2010-2024
Institut National des Sciences Appliquées de Toulouse
2018-2024
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2010-2024
Biotechnology Institute
2017-2023
University of Lisbon
2011
Newcastle University
2006-2010
Université Toulouse III - Paul Sabatier
2010
CAZypedia was initiated in 2007 to create a comprehensive, living encyclopedia of the carbohydrate-active enzymes (CAZymes) and associated carbohydrate-binding modules involved synthesis, modification degradation complex carbohydrates. is closely connected with actively curated CAZy database, which provides sequence-based foundation for biochemical, mechanistic structural characterization these diverse proteins. Now celebrating its 10th anniversary online, successful example dynamic,...
Enzymes that hydrolyze complex carbohydrates play important roles in numerous biological processes result the maintenance of marine and terrestrial life. These enzymes often contain noncatalytic carbohydrate binding modules (CBMs) have substrate-targeting functions. In general, there is a tight correlation between ligands recognized by bacterial CBMs substrate specificity appended catalytic modules. Through high-resolution structural studies, we demonstrate architecture ligand sites 4...
Multifunctional proteins, which play a critical role in many biological processes, have typically evolved through the recruitment of different domains that required functional diversity. Thus activities displayed by these proteins are mediated spatially distinct domains, consistent with specific chemical requirements each activity. Indeed, current evolutionary theory argues colocalization diverse within an enzyme is likely to be rare event, because it would compromise existing activity...
The microbial deconstruction of the plant cell wall is a critical biological process, which also provides important substrates for environmentally sustainable industries. Enzymes that hydrolyze generally contain non-catalytic carbohydrate binding modules (CBMs) contribute to degradation. Here we report biochemical properties and crystal structure family CBMs (CBM60) are located in xylanases. Uniquely, proteins display broad ligand specificity, targeting xylans, galactans, cellulose. Some...
ABSTRACT Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify properties Candida antarctica lipase B. A reliable screening protocol was defined and validated using saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than wild-type enzyme.
The deconstruction of the plant cell wall is an important biological process that attracting considerable industrial interest, particularly in bioenergy sector. Enzymes attack generally contain one or more noncatalytic carbohydrate binding modules (CBMs) play targeting function. While CBMs bind to backbones structural polysaccharides have been widely described, recognize components vast array decorations displayed on these polymers relatively unexplored. Here we show a family 35 CBM member...
The enzymic degradation of plant cell walls plays a central role in the carbon cycle and is increasing environmental industrial significance. catalytic modules enzymes that catalyze this process are generally appended to noncatalytic carbohydrate-binding (CBMs). CBMs potentiate rate catalysis by bringing their cognate into intimate contact with target substrate. A powerful wall-degrading system Clostridium thermocellum multienzyme complex, termed "cellulosome." Here, we identify novel CBM...
The field of plant cell wall biology is constantly growing and consequently so the need for more sensitive specific probes individual components. Xyloglucan a key polysaccharide widely distributed in kingdom both structural storage tissues that exist fucosylated non-fucosylated variants. Presently, only xyloglucan marker available monoclonal antibody CCRC-M1 to terminal α-1,2-linked fucosyl residues on oligo- polysaccharides. As viable alternative searches natural binding proteins or...
The functional screening of a Pseudacanthotermes militaris termite gut metagenomic library revealed an array xylan-degrading enzymes, including P. 25 (Pm25), multimodular glycoside hydrolase family 10 (GH10). Sequence analysis showed details the unusual domain organization this enzyme. It consists one catalytic domain, which is intercalated by two carbohydrate binding modules (CBMs) from 4. genes upstream encoding Pm25 are susC-susD-unk, suggesting Xyn10C-like enzyme belonging to...
Molecular engineering of ligand-binding proteins is commonly used for identification variants that display novel specificities. Using this approach to introduce specificities into CBMs (carbohydrate-binding modules) has not been extensively explored. Here, we report the a CBM, CBM4-2 from Rhodothermus marinus xylanase Xyn10A, and X-2 variant. As compared with wild-type protein, engineered module displays higher specificity polysaccharide xylan, lower preference binding xylo-oligomers rather...
With the growing need for renewable sources of energy, interest enzymes capable biomass degradation has been increasing. In this paper, we consider two different xylanases from GH-11 family: particularly active xylanase Neocallimastix patriciarum, NpXyn11A, and hyper-thermostable mutant environmentally isolated xylanase, EvXyn11TS. Our aim is to identify molecular determinants underlying enhanced capacities these ultimately graft abilities one on other. Molecular dynamics simulations...
Abstract Enzymes are involved in various types of biological processes. In many cases, they part multi-component machineries where enzymes localized close proximity to each-other. such situations, it is still not clear whether inter-enzyme spacing actually plays a role or if the colocalization complementary activities sufficient explain efficiency system. Here, we focus on effect spatial when identical immobilized onto surface. By using an innovative grafting procedure based use two...
Synergism between enzymes is of crucial importance in cell metabolism. This synergism occurs often through a spatial organisation favouring proximity and substrate channelling. In this context, we developed strategy for evaluating the impact geometry two involved nature recycling carbon derived from plant wall polymers. By using an innovative covalent association process protein fragments, Jo In, produced bi-modular chimeric complexes connecting xylanase xylosidase, deconstruction...
Using small-angle neutron scattering (SANS), we examine the structure and conformational behavior of wheat arabinoxylan (AX) prepared at various concentrations in a sodium phosphate aqueous buffer. As for another major hemicellulose, xyloglucan, observe small number large clusters surrounded by AX chains that behave exactly as polymer good solvent with Flory exponent ν = 0.588. The fit data high q-values to standard worm-like chain model gives persistence length lp 45 Å cross section 2Rc...