A5048841797- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Microbial Metabolites in Food Biotechnology
- Glycosylation and Glycoproteins Research
- Animal Nutrition and Physiology
- Biochemical and Molecular Research
- Food composition and properties
- Polysaccharides Composition and Applications
- Protein Hydrolysis and Bioactive Peptides
- Carbohydrate Chemistry and Synthesis
- Biochemical and Structural Characterization
- Muscle metabolism and nutrition
- Enzyme Structure and Function
- Proteins in Food Systems
- RNA and protein synthesis mechanisms
- Protist diversity and phylogeny
- Plant-Microbe Interactions and Immunity
- Moringa oleifera research and applications
- Genomics and Phylogenetic Studies
- Bacterial Genetics and Biotechnology
- Aquaculture Nutrition and Growth
- Antimicrobial Peptides and Activities
- Public Health in Brazil
- Advanced Cellulose Research Studies
University of Lisbon
2014-2024
Nzytech (Portugal)
2014-2024
Centre for Research in Anthropology
2020
Universidade Federal de Mato Grosso
2019
Significance Plant cell wall (PCW) polysaccharide degradation is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Ruminococcus flavefaciens synthesizes highly efficient degrading apparatus. Here, six previously unidentified R. CBM families were identified that targeted β-glucans, β-mannans, pectins. Crystal structures of these CBMs revealed recognition β-glucans β-mannans was...
Animal venoms are large, complex libraries of bioactive, disulphide-rich peptides. These peptides, and their novel biological activities, increasing pharmacological therapeutic importance. However, recombinant expression venom peptides in Escherichia coli remains difficult due to the significant number cysteine residues requiring effective post-translational processing. There is also an urgent need develop high-throughput protocols applicable production reticulated enable efficient screening...
The deconstruction of the plant cell wall is an important biological process that attracting considerable industrial interest, particularly in bioenergy sector. Enzymes attack generally contain one or more noncatalytic carbohydrate binding modules (CBMs) play targeting function. While CBMs bind to backbones structural polysaccharides have been widely described, recognize components vast array decorations displayed on these polymers relatively unexplored. Here we show a family 35 CBM member...
ABSTRACT In general, cellulases and hemicellulases are modular enzymes in which the catalytic domain is appended to one or more noncatalytic carbohydrate binding modules (CBMs). CBMs, by concentrating parental enzyme at their target polysaccharide, increase capacity of module bind substrate, leading a potentiation catalysis. Clostridium thermocellum hypothetical protein Cthe_0821, defined here as C. Man5A, comprising an N-terminal signal peptide, family 5 glycoside hydrolase (GH5) module, 32...
The cloning, expression and characterization of three cellulosomal pectinolytic enzymes viz., two variants PL1 (PL1A PL1B) PL9 from Clostridium thermocellum was carried out. comparison the primary sequences PL1A, PL1B revealed that these proteins displayed considerable sequence similarities with family 1 9 polysaccharide lyases, respectively. are putative catalytic domains protein ABN54148.1 ABN53381.1 These also contain carbohydrate binding module (CBM) type-I dockerin. associated CBM PL1A...
Cellulosomes, synthesized by anaerobic microorganisms such as Clostridium thermocellum, are remarkably complex nanomachines that efficiently degrade plant cell wall polysaccharides. Cellulosome assembly results from the interaction of type I dockerin domains, present on catalytic subunits, and cohesin domains a large non-catalytic integrating protein acts molecular scaffold. In general, dockerins contain two distinct cohesin-binding interfaces appear to display identical ligand...
Anaerobic cellulolytic bacteria organize a comprehensive range of cellulases and hemicellulases in high molecular weight multienzyme complexes termed cellulosomes. Integration cellulosomal components occurs via highly ordered protein-protein interactions between cohesins dockerins. This paper reports the production mini-cellulosomes containing one (GH16-1C) or three (GH16-3C) copies Clostridium thermocellum glucanase 16A (CtGlc16A). Barley β-1,3-1,4-glucans are known to be antinutritive for...
This study investigates the use of recombinant peptidases (EC 3.4) to improve protein hydrolysis and digestibility in Arthrospira platensis, with a focus on addressing challenge reduced bioavailability for monogastric animals due resistant protein-pigment formations, such as phycocyanin, increased digesta viscosity caused by jellification properties. A library 192 was generated, from which 142 soluble were expressed Escherichia coli subsequently screened activity against an A. platensis...
Noncatalytic carbohydrate-binding modules (CBMs), which are found in a variety of carbohydrate-degrading enzymes, have been grouped into sequence-based families. CBMs, by recruiting their appended enzymes onto the surface target substrate, potentiate catalysis particularly against insoluble substrates. Family 6 CBMs (CBM6s) display unusual properties that they present two potential ligand-binding sites termed clefts A and B, respectively. Cleft B is located on concave β-sandwich fold while...
A fibrilação atrial (FA) é uma arritmia cardíaca caracterizada por impulsos elétricos rápidos e desorganizados que se originam nos átrios, levando a batimentos cardíacos irregulares. condição pode resultar de anomalias estruturais no coração, como cardiopatias doenças valvulares, bem fatores não cardíacos, pulmonares crônicas, hipertensão consumo excessivo estimulantes, cafeína álcool. Predisposições genéticas desequilíbrios hormonais, incluindo distúrbios da tireoide, também foram...
Ruminant herbivores meet their carbon and energy requirements from a symbiotic relationship with cellulosome-producing anaerobic bacteria that efficiently degrade plant cell-wall polysaccharides. The assembly of carbohydrate-active enzymes (CAZymes) into cellulosomes enhances protein stability enzyme synergistic interactions. Cellulosomes comprise diverse CAZymes displaying modular architecture in which catalytic domain is connected, via linker sequences, to one or more noncatalytic...
Aim: The present study describes the procedure for isolation, cloning, expression and purification of eight different microbial enzymes required assembly a comprehensive range enzymatic test kits. Methodology: genes encoding were isolated through Polymerase Chain Reaction or synthesized In vitro inserted into variety prokaryotic vectors. resulting recombinant proteins expressed at high levels in Escherichia coli cells purified following chromatographic approaches. catalytic activity each...
To associate food consumption according to the groups that make up pyramid with aerobic and anaerobic performance of Brazilian athletes. A cross-sectional study 168 athletes a mean age BMI 20.84 ± 7.74 years 22.88 3.1 kg/m², respectively. Maximum power output was significantly associated meat eggs (β = 0.31; p < 0.05). VO2max exhibited positive relationship fruit group 0.29; significant inverse relation between legumes observed -0.76; The dairy products had an association -0.43; 0.01)....