A5055081946- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Adipose Tissue and Metabolism
- Polysaccharides and Plant Cell Walls
- Animal Nutrition and Physiology
- Peroxisome Proliferator-Activated Receptors
- Meat and Animal Product Quality
- Microbial Metabolites in Food Biotechnology
- Lipid metabolism and biosynthesis
- Hemoglobinopathies and Related Disorders
- Genetic and phenotypic traits in livestock
- Trace Elements in Health
- Glycosylation and Glycoproteins Research
- Fatty Acid Research and Health
- Iron Metabolism and Disorders
- Ruminant Nutrition and Digestive Physiology
- Genomics and Phylogenetic Studies
- Reproductive Biology and Fertility
- Muscle metabolism and nutrition
- Prion Diseases and Protein Misfolding
- Pharmacological Effects and Assays
- Protein Hydrolysis and Bioactive Peptides
- Toxin Mechanisms and Immunotoxins
- Reproductive System and Pregnancy
- Aquaculture Nutrition and Growth
University of Lisbon
2013-2024
Nzytech (Portugal)
2019-2024
Centre for Research in Anthropology
2020-2022
Fundação de Estudo e Pesquisa em Medicina Veterinária e Zootecnia
2016
University of Southampton
2016
Cambridge University Press
2016
New York University Press
2016
Instituto de Investigação Científica Tropical
2004
University of Veterinary Medicine Vienna
2001
Universidade Federal Fluminense
1996-1997
Significance Plant cell wall (PCW) polysaccharide degradation is an important biological and industrial process. Noncatalytic carbohydrate binding modules (CBMs) fulfill a critical targeting function in PCW depolymerization. Ruminococcus flavefaciens synthesizes highly efficient degrading apparatus. Here, six previously unidentified R. CBM families were identified that targeted β-glucans, β-mannans, pectins. Crystal structures of these CBMs revealed recognition β-glucans β-mannans was...
The microbial degradation of the plant cell wall is an important biological process, representing a major component carbon cycle. Enzymes that mediate hydrolysis this composite structure are modular proteins contain non-catalytic carbohydrate binding modules (CBMs) enhance catalytic activity. CBMs grouped into sequence-based families, and in previous study we showed family 6 CBM (CBM6) interacts with xylan contains two potential ligand clefts, designated cleft A B. Mutagenesis NMR studies...
Modular glycoside hydrolases that attack recalcitrant polymers generally contain noncatalytic carbohydrate-binding modules (CBMs), which play a critical role in the action of these enzymes by localizing appended catalytic domains onto surface insoluble polysaccharide substrates. Type B CBMs, recognize single chains, display ligand specificities are consistent with substrates hydrolyzed associated domains. In multiple distinct substrate specificities, it is unclear how different activities...
Glycoside hydrolases that release fixed carbon from the plant cell wall are of considerable biological and industrial importance. These contain non-catalytic carbohydrate binding modules (CBMs) that, by bringing appended catalytic domain into intimate association with its insoluble substrate, greatly potentiate catalysis. Family 6 CBMs (CBM6) highly unusual because they two distinct clefts (cleft A cleft B) potentially can function as sites. Henshaw et al. (Henshaw, J., Bolam, D. N., Pires,...
Multifunctional proteins, which play a critical role in many biological processes, have typically evolved through the recruitment of different domains that required functional diversity. Thus activities displayed by these proteins are mediated spatially distinct domains, consistent with specific chemical requirements each activity. Indeed, current evolutionary theory argues colocalization diverse within an enzyme is likely to be rare event, because it would compromise existing activity...
Iron is essential in the retina because heme-containing enzyme guanylate cyclase modulates phototransduction rods and cones. Transferrin endocytosis classical pathway for obtaining iron from blood circulation retina. However, storage protein ferritin has been also recently proposed as an carrier. In this study, presence of Scara5 its binding to L-ferritin was investigated Our results showed that Scara5, specific receptor L-ferritin, expressed mouse human retinas many cell types, including...
The present study assessed the effect of pig genotype (fatty v. lean) and dietary protein lysine (Lys) levels (normal reduced) on intramuscular fat (IMF) content, subcutaneous adipose tissue (SAT) deposition, fatty acid composition mRNA genes controlling lipid metabolism. experiment was conducted sixty intact male pigs (thirty Alentejana purebred thirty Large White × Landrace Pietrain crossbred), from 60 to 93 kg live weight. Animals were divided into three groups fed with following diets:...
Abstract Background Both genetic background and finishing system can alter fat deposition, thus indicating their influence on adipogenic lipogenic factors. However, the molecular mechanisms underlying deposition fatty acid composition in beef cattle are not fully understood. This study aimed to assess effect of breed dietary silage level expression patterns key genes controlling lipid metabolism subcutaneous adipose tissue (SAT) longissimus lumborum (LL) muscle cattle. To that purpose, forty...
Meat from lambs finished with high-starch diets often contains low concentration of vaccenic (t11-18:1) and rumenic (c9,t11-18:2) acids high t10-18:1. We hypothesized that replacing cereals by dehydrated citrus pulp (DCP) the inclusion tanniferous feed sources in oil supplemented might reduce accumulation t10-18:1 increase t11-18:1 c9,t11-18:2 lamb meat, without affecting productive performance. In total, 32 were assigned to four which combine two factors: basal diet (BD) (cereals v. DCP)...
The cumulative effects of dietary arginine, leucine and protein levels on fat content, fatty acid composition mRNA genes controlling lipid metabolism in pig longissimus lumborum muscle subcutaneous adipose tissue (SAT) were investigated. experiment was performed fifty-four intact male pigs (Duroc × Pietrain Large White Landrace crossbred), with a live weight ranging from 59 to 92 kg. randomly assigned one six experimental treatments ( n 9). followed 2 3 factorial arrangement, two arginine...
In ruminants, unsaturated dietary fatty acids are biohydrogenated in the rumen and further metabolised various tissues, including liver, which has an important role lipid lipoprotein metabolism. Therefore, manipulation of muscle acid composition should take into account liver present study, influence breed diet on gene expression was investigated order to clarify this organ metabolism ruminants. Forty purebred young bulls from two phylogenetically distant autochthonous cattle breeds,...
The effects of feeding Cistus ladanifer (Cistus) and a blend soybean linseed oil (1 : 2 vol/vol) on fatty acid (FA) composition lamb meat lipids messenger RNA (mRNA) expression desaturase enzymes was assessed. In total, 54 male lambs were randomly assigned to 18 pens nine diets, resulting from the combination three inclusion levels (50 v. 100 200 g/kg dry matter (DM)) (0 40 80 DM). forage-to-concentrate ratio diets 1 1. Longissimus muscle extracted, fractionated into neutral (NL) polar lipid...
Abstract During the course of evolution, cellulosome, one Nature’s most intricate multi-enzyme complexes, has been continuously fine-tuned to efficiently deconstruct recalcitrant carbohydrates. To facilitate uptake released sugars, anaerobic bacteria use highly ordered protein-protein interactions recruit these nanomachines cell surface. Dockerin modules located within a non-catalytic macromolecular scaffold, whose primary role is assemble cellulosomal enzymatic subunits, bind cohesin...
Abstract The isolated or combined effects of betaine and arginine supplementation reduced protein diets (RPD) on fat content, fatty acid composition mRNA levels genes controlling lipid metabolism in pig m. longissimus lumborum subcutaneous adipose tissue (SAT) were assessed. experiment was performed forty intact male pigs (Duroc×Large White×Landrace cross-breed) with initial final live weights 60 93 kg, respectively. Pigs randomly assigned to one the following five ( n 8): 16·0 % crude...
Reduced protein diets affects the amounts of proteins related to fibre type and structure energy.
The intramuscular fat composition of ruminant meats influences the quality final product, which explains increasing interest in assessing fatty acid profile meat from different production systems. In this study, it was hypothesized that there are breed- and diet-induced variations on lipid metabolism muscle, may be, at least partially, modulated by stearoyl-CoA desaturase (SCD) gene expression levels. Forty purebred young bulls two phylogenetically distant autochthonous cattle breeds,...
Abstract The influence of genotype (lean v. fatty) and dietary protein level (normal reduced) on plasma metabolites, hepatic fatty acid composition mRNA levels lipid-sensitive factors is reported for the first time, using pig as an experimental model. experiment was conducted forty entire male pigs (twenty lean Large White×Landrace×Pietrain cross-breed twenty Alentejana purebreed) from 60 to 93 kg live weight. Each divided into two subgroups, which were fed following diets: a normal diet...
The production of pork with high amounts intramuscular fat (IMF) without an increase in subcutaneous is highly desirable for the pig industry and consumers. Herein, we question impact dietary protein reduction (18% v. 13%) on deposition adipose tissue (SAT) longissimus lumborum (LL) muscle using genetically diverse pigs body fatness (lean fat). A clear effect genotype was observed plasma insulin (P=0.004) leptin (P<0.001), as well backfat thickness having higher values. Accordingly, IMF...