A5000592099- Ion Transport and Channel Regulation
- Adipose Tissue and Metabolism
- Lipid metabolism and biosynthesis
- Fungal and yeast genetics research
- Fatty Acid Research and Health
- Inflammatory Bowel Disease
- Neonatal Respiratory Health Research
- Plant nutrient uptake and metabolism
- Endoplasmic Reticulum Stress and Disease
- Cancer-related Molecular Pathways
- Dietary Effects on Health
- ATP Synthase and ATPases Research
- Electrolyte and hormonal disorders
- Circadian rhythm and melatonin
- Diet, Metabolism, and Disease
- Immune cells in cancer
- Gestational Diabetes Research and Management
- Peroxisome Proliferator-Activated Receptors
- Photosynthetic Processes and Mechanisms
- Lanthanide and Transition Metal Complexes
- Mitochondrial Function and Pathology
- Reproductive System and Pregnancy
- Membrane-based Ion Separation Techniques
- Liver Disease Diagnosis and Treatment
- Biomarkers in Disease Mechanisms
Institut d'Investigació Sanitària Pere Virgili
2017-2024
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2017-2024
Instituto de Salud Carlos III
2017-2024
Hospital Universitari Joan XXIII de Tarragona
2023
Universitat Rovira i Virgili
2017-2019
University of Lisbon
2013-2016
Institute for Biotechnology and Bioengineering
2012
Rede de Química e Tecnologia
2007-2010
Institute for Research in Biomedicine
2010
Universidade do Porto
2003
ABSTRACT Objective For a long time Aquaporin‐7 has been the only aquaporin associated with adipose tissue, and its dysregulation linked to underlying mechanisms of obesity. However, presence alternative glycerol channels within tissue postulated, which prompted us search alternate transport routes in adipocytes. In view this, it is hypothesized that Aquaporin‐11 (AQP11) would have role adipocyte cell biology. Methods The expression, localization function human AQP11 (hAQP11) cultured...
Succinate and succinate receptor 1 (SUCNR1) are linked to fibrotic remodeling in models of non-alcoholic fatty liver disease (NAFLD), but whether they have roles beyond the activation hepatic stellate cells remains unexplored. We investigated succinate/SUCNR1 axis context NAFLD specifically hepatocytes.We studied phenotype wild-type Sucnr1-/- mice fed a choline-deficient high-fat diet induce steatohepatitis (NASH), explored function SUCNR1 murine primary hepatocytes human HepG2 treated with...
Crohn's disease (CD) is characterized by the expansion of mesenteric fat, also known as "creeping fat." We explored plasticity and immune properties adipose-derived stem cells (ASCs) in context CD potential key players development creeping fat. Mesenteric CD-derived ASCs presented a more proliferative, inflammatory, invasive, phagocytic phenotype than equivalent from healthy donors, irrespective clinical stage. Remarkably, subcutaneous depot patients with showed an activated response that...
The plasma membrane aquaporin-7 (AQP7) has been shown to be expressed in adipose tissue and its role glycerol release/uptake adipocytes postulated correlated with obesity onset. However, some studies have contradicted this view. Based on situation, we re-assessed the precise localization of AQP7 analyzed function as a water and/or channel cells. Fractionation mice revealed that is located both stromal vascular fractions. Moreover, was only aquaglyceroporin 3T3-L1 adipocytes. By...
Abstract Aquaporins (AQPs) are membrane water/glycerol channels that involved in many physiological functions. Aquaporin‐based modulators predicted to have potential utility the treatment of several diseases, as well chemical tools assess AQPs function biological systems. We recently reported gold(III) compounds human AQP3 inhibitors, with Auphen most potent series. In this work, we assessed modulation aquaporin‐7 (AQP7) expressed an adipocyte cell model and show significantly inhibits mouse...
Debaryomyces hansenii is a halotolerant yeast that produces and assimilates wide variety of polyols. In this work we evaluate polyol transport in D. CBS 767, detecting the occurrence polyol/H+ (and sugar/H+) symporter activity, through transient extracellular alkalinization unbuffered starved cell suspensions. From genome database, selected nine ORFs encoding putative transporter proteins to clone centromeric plasmid with C-terminal GFP tagging screened for symporters by heterologous...
The COVID-19 pandemic has thrown the entire world into economic and mental turmoil, changed our lifestyles way we interact in care what is a very challenging rapidly evolving circumstance. methodology: this bibliographical research of documentary, descriptive qualitative nature, information was obtained from analysis scientific data published on online platforms. Results Discussion: Healthcare professionals face additional stressors. They are known to include an increased risk infection,...
Plasma membrane integrity, ability to transport substrates and maintenance of homeostasis represent obligatory requirements for efficient ethanol production by Saccharomyces cerevisiae. The effect on water diffusion through the bilayer mediated movements was evaluated stopped flow spectroscopy. Ethanol stimulated inhibited transport. In a strain overexpressing AQY1, activation energy increased progressively (from 5.9 12.7 kcal mol(-1)) increasing concentrations (up 12% v/v), indicating that...
Abstract Aquaporins (AQPs) are membrane channels widely distributed in nature. Typically, multiple isoforms expressed a single tissue. The adipose tissue is no exception where several AQP members have been identified. importance of overlapped AQPs expression unclear, yet interisoforms interactions might be required for key cellular functions. Recently, AQP5 was described as regulator other isoforms. Therefore, we hypothesized role adipocyte biology. Gene analysis revealed the presence both...
Abstract Purpose Recent studies point to adipose-derived stem cells (ASCs) as a link between obesity and cancer. We aimed determine whether survivin, which is highly secreted by ASCs from subjects with obesity, might drive pro-tumoral phenotype in macrophages. Methods The effect of ASC conditioned medium on the macrophage was assessed expression studies. Survivin intracellular localization internalization were examined subcellular fractionation immunofluorescence, respectively. Loss-...
Coronavirus-19 (COVID-19) disease is driven by an unchecked immune response to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus which alters host mitochondrial-associated mechanisms. Compromised mitochondrial health results in abnormal reprogramming of glucose metabolism, can disrupt extracellular signalling. We hypothesized that examining energy-related signalling metabolites implicated SARS-CoV-2 infection would provide potential biomarkers for predicting risk...
Intact yeast cells loaded with 5- and-6-carboxyfluorescein were used to assess water transport. The results similar those previously reported for protoplasts assessed by using either fluorescence or light scattering, and the activation energies 8.0 15.1 kcal mol(-1) (33.4 63.2 kJ mol(-1)) a strain overexpressing AQY1 aquaporin parental strain, respectively.
Abstract Background Metabolic reprogramming and abnormal glucose metabolism are hallmarks of head neck squamous cell carcinoma (HNSCC). Certain oncogenes can promote cancer‐related metabolic changes, but understanding their crosstalk in HNSCC biology treatment is essential for identifying predictive biomarkers developing target therapies. Methods We assessed the value survivin/BIRC5 as a radioresistance factor potentially modulated by predicting therapeutic sensitivity prognosis cohort 32...
Dyslipidemia in gestational diabetes has been associated with worse perinatal outcomes. The ANGPTL3-4-8 axis regulates lipid metabolism, especially the transition from fasting to feeding. In this study, we evaluated response of ANGPTL3, 4, and 8 after intake a mixed meal women normal glucose tolerance diabetes, assessed their gene expressions different placental locations. Regarding circulating levels 8, observed an absence ANGPTL4 GDM group compared its presence control group. At level,...
In this study, we hypothesized that trans ( t )8, cis c )10 conjugated linoleic acid (CLA) isomer possesses anti‐adipogenic properties in resemblance to its structural analog, the 10, 12 CLA isomer. After chemical synthesis and purification of 8, 10 our laboratory, report, for first time, individual action on lipid metabolism 3T3‐L1 adipocytes. Cells were supplemented with 50 μM (LA), 12, or during 11 days differentiation. Intracellular triacylglycerols (TAG) content was affected by fatty...
Our understanding of the interplay between human adipose tissue and immune system is limited. The mesothelium, an immunologically active structure, emerged as a source visceral tissue. After investigating mesothelial properties subcutaneous their progenitors, we explored whether dysfunctional obese Crohn’s disease environments influence mesothelial/mesenchymal adipocyte precursors, well ability to mount response. Using tandem transcriptomic/proteomic approach, evaluated mesenchymal...