A5015068416- Mass Spectrometry Techniques and Applications
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Analytical Chemistry and Chromatography
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Seaweed-derived Bioactive Compounds
- Microbial Metabolites in Food Biotechnology
- Carbohydrate Chemistry and Synthesis
- Metabolomics and Mass Spectrometry Studies
- Genomics and Phylogenetic Studies
- Advanced Proteomics Techniques and Applications
- Polysaccharides Composition and Applications
- Enzyme Catalysis and Immobilization
- Marine and coastal plant biology
- Algal biology and biofuel production
- Enzyme-mediated dye degradation
- Fermentation and Sensory Analysis
- Plant Reproductive Biology
- Advanced Chemical Sensor Technologies
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Mycorrhizal Fungi and Plant Interactions
- Fungal and yeast genetics research
- Lignin and Wood Chemistry
Biopolymères Interactions Assemblages
2015-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2010-2024
École Nationale Supérieure de Chimie de Rennes
2021
Centre National de la Recherche Scientifique
2021
Institut Lumière Matière
2021
Université de Rennes
2021
Université Claude Bernard Lyon 1
2021
Institut National de la Recherche Agronomique
2012-2015
Université de Limoges
2008
Université Sorbonne Paris Nord
2008
The understanding of enzymatic polysaccharide degradation has progressed intensely in the past few years with identification a new class fungal-secreted enzymes, lytic monooxygenases (LPMOs) that enhance cellulose conversion. In fungal kingdom, saprotrophic fungi display high number genes encoding LPMOs from family AA9 but functional relevance this redundancy is not fully understood.In study, we investigated set identified secretomes coprophilous ascomycete Podospora anserina, biomass...
Abstract Macroalgae contribute substantially to primary production in coastal ecosystems. Their biomass, mainly consisting of polysaccharides, is cycled into the environment by marine heterotrophic bacteria using largely uncharacterized mechanisms. Here we describe complete catabolic pathway for carrageenans, major cell wall polysaccharides red macroalgae, bacterium Zobellia galactanivorans . Carrageenan catabolism relies on a multifaceted carrageenan-induced regulon, including non-canonical...
Abstract Imbibed seeds of the Arabidopsis thaliana accession Djarly are affected in mucilage release from seed coat epidermal cells. The impaired locus was identified as a pectin methylesterase inhibitor gene, PECTIN METHYLESTERASE INHIBITOR6 (PMEI6), specifically expressed cells at time when polysaccharides accumulated. This spatio-temporal regulation appears to be modulated by GLABRA2 and LEUNIG HOMOLOG/MUCILAGE MODIFIED1, expression PMEI6 is reduced mutants these transcription regulators....
Abstract The enzymatic conversion of plant biomass has been recently revolutionized by the discovery lytic polysaccharide monooxygenases (LPMOs) that carry out oxidative cleavage polysaccharides. These very powerful enzymes are abundant in fungal saprotrophs. LPMOs require activation electrons can be provided cellobiose dehydrogenases (CDHs), but as some fungi lack CDH-encoding genes, other recycling must exist. We investigated ability AA3_2 flavoenzymes secreted under lignocellulolytic...
There is increasing biopharmaceutical interest in oligosaccharides and glycosylation. A key requirement for these sample types the ability to characterize chain length, branching, type of monomers, importantly stereochemistry anomeric configuration. Herein, we showcase multi-function capability a cyclic ion mobility (cIM) separator embedded quadrupole/time-of-flight mass spectrometer (Q-ToF MS). The instrument design enables selective activation mobility-separated precursors followed by cIM...
A large number of retaining glycosidases catalyze both hydrolysis and transglycosylation reactions, but little is known about what determines the balance between these two activities (transglycosylation/hydrolysis ratio). We previously obtained by directed evolution mutants F401S N282T Thermus thermophilus β-glycosidase (Ttβ-gly, glycoside hydrolase family 1 (GH1)), which display a higher transglycosylation/hydrolysis ratio than wild-type enzyme. In order to find cause activity...
Arabinoxylans (AX) and (1→3),(1→4)-β-glucans (BG) are the major components of wheat grain cell walls. Although incompletely described at molecular level, it is known that chemical distributional heterogeneity these compounds impacts quality use wheat. In this work, an emerging technique based on MALDI mass spectrometry imaging (MSI) was employed to map variations in quantity, localization, structure polysaccharides endosperm during maturation. MSI couples detailed structural information with...
Collision cross section (CCS) databases based on single-laboratory measurements must be cross-validated to extend their use in peak annotation. This work addresses the validation of first comprehensive TWCCSN2 database for steroids. First, its long-term robustness was evaluated (i.e., a year and half after generation; Synapt G2-S instrument; bias within ±1.0% 157 ions, 95.7% total ions). It further by three external laboratories, including two different TWIMS platforms G2-Si Vion IMS QToF;...
The enzymatic conversion of plant biomass has been recently revolutionized by the discovery lytic polysaccharide monooxygenases (LPMO) that catalyze oxidative cleavage polysaccharides. These powerful enzymes are secreted a large number fungal saprotrophs and important components commercial enzyme cocktails used for industrial conversion. Among 33 AA9 LPMOs encoded genome Podospora anserina, PaLPMO9H catalyzes mixed C1/C4 cellulose cello-oligosaccharides. Activity on several hemicelluloses...
Carbohydrate isomers with identical atomic composition cannot be distinguished by mass spectrometry. By separating the ions according to their conformation in gas phase, ion mobility (IM) coupled spectrometry is an attractive approach overcome this issue and extend limits of structural glycosciences. Recent technological developments have significantly increased resolving power separators. One such instrument features a cyclic traveling-wave IM separator integrated quadrupole/time-of-flight...
Compared to other analytical methods, matrix‐assisted laser desorption ionization mass spectrometry (MALDI‐MS) presents several unique advantages for the structural characterization of degradation products carbohydrates. Our final goal is implement this technique as a high‐throughput platform, with aim exploring natural bio‐diversity discover new carbohydrate depolymerizing enzymes. In approach, variety carbohydrates will be used enzymes substrates and MALDI‐MS employed monitor...
Three series of model homogalacturonans (HGs) covering a large range degree methylesterification (DM) were prepared by chemical and/or enzymatic means. Randomly demethylesterified HGs, HGs containing few long galacturonic acid stretches, and with numerous but short blocks recovered. The analysis the degradation products generated action purified pectin lyase allowed definition two new parameters, blockiness, absolute blockiness highly methylesterified stretches (DBMe DB(abs)Me,...
Cell-to-cell adhesion is essential for establishment of multicellularity. In plants, such mediated through a middle lamella composed primarily pectic polysaccharides. The molecular interactions that influence cell-to-cell are not fully understood. We have used Arabidopsis (Arabidopsis thaliana) seed coat mucilage as model system to investigate between cell wall carbohydrates. Using forward-genetic approach, we discovered gene, RUBY PARTICLES IN MUCILAGE (RUBY), encoding protein annotated...
Carbohydrates are complex structures that still challenge analysts today because of their different levels isomerism, notably the anomerism glycosidic bond. It has been shown recently is preserved upon gas-phase fragmentation and high-resolution ion mobility (IMS) can distinguish anomers. However, these concepts have yet to be applied biological products. We used IMS on a cyclic device characterize reaction products Uhgb_MS, novel mannoside synthase GH130 family. designed so-called IMSn...
The structural characterization of oligosaccharides still challenges the field analytical chemistry. Tandem mass spectrometry offers many advantages toward this aim, although generic fragmentation method (low-energy collision-induced dissociation) shows clear limitations and is often insufficient to retrieve some essential information on these molecules. In work, we present first application helium charge transfer dissociation (He-CTD) characterize structure complex oligosaccharides. We...