A5017541593- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Gut microbiota and health
- Selenium in Biological Systems
- Clostridium difficile and Clostridium perfringens research
- Trace Elements in Health
- Pharmacological Effects of Natural Compounds
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Enzyme Production and Characterization
- RNA modifications and cancer
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- MRI in cancer diagnosis
- Burn Injury Management and Outcomes
- Asymmetric Hydrogenation and Catalysis
- Cardiac Arrest and Resuscitation
- Forensic Entomology and Diptera Studies
- Protein Structure and Dynamics
- Protein purification and stability
- Plant Pathogenic Bacteria Studies
- Climate Change and Health Impacts
Universität Greifswald
2014-2024
Helmholtz-Zentrum Berlin für Materialien und Energie
2009-2010
Fraunhofer Institute for Telecommunications, Heinrich Hertz Institute
2007
Kiel University
1974
Successful proteome analyses of highly dilute samples are strongly dependent on optimized workflows considering especially sample preparation prior to sensitive mass spectrometric analysis. Various methods available for enrichment samples, each characterized by specific advantages and disadvantages limiting their general application as a method choice. Here we suggest an universal protocol ensuring reproducibility effective commercial affinity beads. By comparably assessing the performance...
The identification of proteins below approximately 70-100 amino acids in bottom-up proteomics is still a challenging task due to the limited number peptides generated by proteolytic digestion. This includes short open reading frame-encoded (SEPs), which are subset small that were not previously annotated or alternatively encoded. Here, we systematically investigated use multiple proteases (trypsin, chymotrypsin, LysC, LysargiNase, and GluC) GeLC-MS/MS analysis improve sequence coverage...
The application of radionuclides for the localization essential trace elements in vivo and characterization their binding proteins is a story intermittently made improvements techniques used detection. In this study we present use neutron activation analysis different autoradiographic imaging methods including real-time digital autoradiography to reveal new insights hierarchy selenium homeostasis. Selenoproteins containing element play important roles CNS. Although CNS does not show highest...
In contrast to extensively studied prokaryotic 'small' transcriptomes (encompassing all small noncoding RNAs), proteomes (here defined as including proteins ≤70 aa) are only now entering the limelight. The absence of a complete protein catalogue in most prokaryotes precludes our understanding how these molecules affect physiology. So far, archaeal genomes have not yet been analyzed broadly with dedicated focus on proteins. Here, we present combinatorial approach, integrating experimental...
Abstract Aldehyde können ohne Isolierung von Zwischenprodukten in Nitrile übergeführt werden, indem ihre Oxime Gegenwart Kupfer(II)‐Ionen und Triäthylamin mit Hilfe Carbodiimiden dehydratisiert werden. Der gesamte Prozeß erfordert bei Raumtemperatur durchweg 3 Stunden. Das Verfahren versagt, wenn die Oximbildung gestört ist oder das Oxim Konkurrenzreaktionen eingeht.
PrsA is an extracytoplasmic folding catalyst essential in Bacillus subtilis. Overexpression of the native from B. subtilis has repeatedly lead to increased amylase yields. Nevertheless, little known about how overexpression heterologous PrsAs can affect secretion.In this study, final yield five extracellular alpha-amylases was by co-expression up 2.5 fold. The effect on alpha-amylase secretion specific co-expressed alpha-amylase. Co-expression a significantly reduce stress response....
Like eukaryotes, different bacterial species express one or more Ser/Thr kinases and phosphatases that operate in various signaling networks by catalyzing phosphorylation dephosphorylation of proteins can immediately regulate biochemical pathways altering protein function. The human pathogen Streptococcus pneumoniae encodes a single kinase-phosphatase couple known as StkP-PhpP, which has shown to be crucial the regulation cell wall synthesis division. In this study, we applied proteomics...
Small open reading frame encoded proteins (SEPs) gained increasing interest during the last few years because of their broad range important functions in both prokaryotes and eukaryotes. In bacteria, signaling, virulence, regulation enzyme activities have been associated with SEPs. Nonetheless, number SEPs detected large-scale proteome studies is often low as classical methods are biased toward identification larger proteins. Here, we present a workflow that allows enhanced small compared to...
Cell division is a central mechanism of life and essential for growth development. Members the Bacteria Eukarya have different mechanisms cell division, which been studied in detail.
Clostridioides difficile infections (CDIs) remain a healthcare problem due to high rates of relapsing/recurrent CDIs (rCDIs). Breakdown colonization resistance promoted by broad-spectrum antibiotics and the persistence spores contribute rCDI. Here, we demonstrate antimicrobial activity natural product class chlorotonils against C. difficile. In contrast vancomycin, chlorotonil A (ChA) efficiently inhibits disease prevents rCDI in mice. Notably, ChA affects murine porcine microbiota lesser...
has been extensively used as a microbial cell factory for industrial enzymes due to its excellent capacities protein secretion and large-scale fermentation. This bacterium is also an attractive host biopharmaceutical production. However, the potential of this organism not fully utilized yet, mostly limited understanding critical rearrangements in membrane proteome upon high-level secretion. Recently, it was shown that bottlenecks heterologous can be resolved by genome minimization. Here, we...
Clostridioides difficile infections have become a major challenge in medical facilities. The bacterium is capable of spore formation allowing the survival antibiotic treatment. Therefore, research on physiology C. important for development alternative treatment strategies. In this study, we investigated eight putative flavodoxins 630. Flavodoxins are small electron transfer proteins specifically low potential. unusually high number suggests that they expressed under different conditions. We...
ABSTRACT Understanding cellular mechanisms of stress management relies on omics data as a valuable resource. However, the lack absolute quantitative protein abundances remains significant limitation, particularly when comparing across different cell compartments. In this study, we aimed to gain deeper insights into proteomic responses Gram-positive model bacterium Bacillus subtilis disulfide stress. We determined proteome-wide abundances, focusing sub-cellular locations (cytosol and...
Clostridioides difficile is the leading cause of antibiotic-associated diarrhea but can also result in more serious, life-threatening conditions. The incidence C. infections hospitals increasing, both frequency and severity, antibiotic-resistant strains are advancing. Against this background antimicrobial peptides (AMPs) an interesting alternative to classic antibiotics. Information on effects AMPs will not only enhance knowledge for possible biomedical application may provide insights into...
The widely conserved twin-arginine translocases (Tat) allow the transport of fully folded cofactor-containing proteins across biological membranes. In doing so, these serve different functions ranging from energy conversion to cell division. Gram-positive soil bacterium Bacillus subtilis, Tat machinery is essential for effective growth in media lacking iron or NaCl. It was previously shown that this phenomenon relates Tat-dependent export heme-containing peroxidase EfeB, which converts Fe2+...
The field of systems biology has been rapidly developing in the past decade. However, data produced by "omics" approaches is lagging behind requirements this field, especially when it comes to absolute abundances membrane proteins. In present study, a novel approach for large-scale quantification challenging subset proteins established and evaluated using osmotic stress management Gram-positive model bacterium Bacillus subtilis as proof-of-principle precedent. Selected were labeled SNAP-tag,...
The function and mode of action small regulatory RNAs is currently still understudied in archaea. In the halophilic archaeon Haloferax volcanii , a plethora sRNAs have been identified; however, in-depth functional analysis missing for most them. We selected RNA (s479) from detailed characterization. sRNA gene encoded between CRISPR locus Cas protein cluster, s479 deletion strain viable was characterized detail. Transcriptome studies wild-type cells mutant revealed upregulation six genes...
To evaluate the influence of blood-brain barrier on neuronal gadolinium deposition in a mouse model after multiple intravenous applications linear contrast agent gadodiamide. The prospective study held 54 mice divided into three groups: healthy (A), with iatrogenic induced disturbance by glioblastoma (B) or cerebral infarction (C). In each group 9 animals received 10 iv-injections gadodiamide (1.2 mmol/kg) every 48 h followed plain T1-weighted brain MRI. A final MRI was performed 5 days last...