A5047320723- Advanced Proteomics Techniques and Applications
- Bacteriophages and microbial interactions
- Mass Spectrometry Techniques and Applications
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Microbial Community Ecology and Physiology
- Streptococcal Infections and Treatments
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Escherichia coli research studies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Neonatal and Maternal Infections
- Microbial Metabolic Engineering and Bioproduction
- Influenza Virus Research Studies
- Vibrio bacteria research studies
- Respiratory viral infections research
- Geomagnetism and Paleomagnetism Studies
- Magnetic and Electromagnetic Effects
- Geology and Paleoclimatology Research
- Metabolomics and Mass Spectrometry Studies
- Molecular Biology Techniques and Applications
- Gene expression and cancer classification
- Enzyme Structure and Function
- Infective Endocarditis Diagnosis and Management
- Protist diversity and phylogeny
Universitätsmedizin Greifswald
2023-2025
Universität Greifswald
2016-2024
Czech Academy of Sciences, Institute of Microbiology
2023
Max Planck Institute for Marine Microbiology
2023
University of Bremen
2023
Abstract Algal blooms produce large quantities of organic matter that is subsequently remineralised by bacterial heterotrophs. Polysaccharide a primary component algal biomass. It has been hypothesised individual heterotrophic niches during are in part determined the available polysaccharide substrates present. Measurement expression TonB-dependent transporters, often specific for uptake, might serve as proxy assessing consumption over time. To investigate this, we present here...
Bacterial pathogens do not only succeed in breaking the barriers that protect humans from infection, but they also manage to evade insults human immune system. The importance of present study resides fact protein citrullination is shown represent a new bacterial mechanism for evasion. In particular, oral pathogen P. gingivalis employs this defuse innate responses by secreting protein-citrullinating enzyme. Of note, finding impacts global health problem periodontitis, it extends prevalent...
Abstract Phytoplankton blooms provoke bacterioplankton blooms, from which bacterial biomass (necromass) is released via increased zooplankton grazing and viral lysis. While consumption of algal during well-studied, little known about the concurrent recycling these substantial amounts necromass. We demonstrate that biomass, such as alpha-glucan storage polysaccharides, generated organic matter, reused thus itself a major carbon source in vitro diatom-dominated bloom. highlight conserved...
Abstract Summary Proteome studies frequently encounter challenges in down-stream data analysis due to limited bioinformatics resources, rapid generation, and variations analytical methods. To address these issues, we developed SpectroPipeR, an R package designed streamline tasks provide a comprehensive, standardized pipeline for Spectronaut® DIA-MS data. This novel automates various processes, including XIC plots, ID rate summary, normalization, batch covariate adjustment, relative protein...
Small open reading frame encoded proteins (SEPs) gained increasing interest during the last few years because of their broad range important functions in both prokaryotes and eukaryotes. In bacteria, signaling, virulence, regulation enzyme activities have been associated with SEPs. Nonetheless, number SEPs detected large-scale proteome studies is often low as classical methods are biased toward identification larger proteins. Here, we present a workflow that allows enhanced small compared to...
Many strains of Escherichia coli carry a 29,250-bp ETT2 pathogenicity island (PAI), which includes genes predicted to encode type III secretion system (T3SS) components. Because it is similar the Salmonella 1 (SPI-1) system, encoding T3SS in enterica, was assumed that also encodes injecting effectors into host cells. This assumption checked E. serotype O2-associated with urinary tract infections and septicemia-which has an intact gene cluster, contrast most this cluster carries deletions...
Extraintestinal pathogenic Escherichia coli (ExPEC) strains constitute a serious and emerging clinical problem, as they cause variety of infections are usually highly antibiotic resistant. Many ExPEC capable evading the bactericidal effects serum causing sepsis. One critical factor for development septicemia is increased survival (iss) gene, which correlated with complement resistance lethality. Although it very important, function iss gene has not been elucidated so far. We have studying...
Abstract In proteomics, fast, efficient, and highly reproducible sample preparation is of utmost importance, particularly in view fast scanning mass spectrometers enabling analyses large series. To address this need, we have developed the web application MassSpecPreppy that operates on open science OT‐2 liquid handling robot from Opentrons. This platform can prepare up to 96 samples at once, performing tasks like BCA protein concentration determination, digestion with normalization,...
The environmental bacterium Legionella pneumophila causes the pneumonia Legionnaires' disease. opportunistic pathogen forms biofilms and employs Icm/Dot type IV secretion system (T4SS) to replicate in amoebae macrophages. A regulatory network comprising quorum sensing (Lqs) transcription factor LvbR controls bacterial motility, virulence biofilm architecture. Here we show by comparative proteomics that formed L. ΔlqsR or ΔlvbR mutants abundance of proteins encoded a genomic 'fitness island',...
has been extensively used as a microbial cell factory for industrial enzymes due to its excellent capacities protein secretion and large-scale fermentation. This bacterium is also an attractive host biopharmaceutical production. However, the potential of this organism not fully utilized yet, mostly limited understanding critical rearrangements in membrane proteome upon high-level secretion. Recently, it was shown that bottlenecks heterologous can be resolved by genome minimization. Here, we...
The Twin-arginine translocation (Tat) pathway of Escherichia coli has great potential for the export biopharmaceuticals to periplasm due its ability transport folded proteins, and proofreading mechanism that allows correctly proteins translocate. Coupling Tat-dependent protein secretion with formation disulfide bonds in cytoplasm E. CyDisCo provides a powerful platform production industrially challenging proteins. In this study, we investigated effects on cells exporting substrate (scFv)...
Streptococcus pyogenes (group A streptococci; GAS) is the main causative pathogen of monomicrobial necrotizing soft tissue infections (NSTIs). To resist immuno-clearance, GAS adapt their genetic information and/or phenotype to surrounding environment. Hyper-virulent streptococcal pyrogenic exotoxin B (SpeB) negative variants caused by covRS mutations are enriched during infection. key driving force for this process bacterial Sda1 DNase.Bacterial infiltration, immune cell influx, necrosis and...
Clostridioides difficile is the leading cause of antibiotic-associated diarrhea but can also result in more serious, life-threatening conditions. The incidence C. infections hospitals increasing, both frequency and severity, antibiotic-resistant strains are advancing. Against this background antimicrobial peptides (AMPs) an interesting alternative to classic antibiotics. Information on effects AMPs will not only enhance knowledge for possible biomedical application may provide insights into...
Hallmarks of cystic fibrosis (CF) are increased viscosity mucus and impaired mucociliary clearance within the airways due to mutations conductance regulator gene. This facilitates colonization lung by microbial pathogens concomitant establishment chronic infections leading tissue damage, reduced function, decreased life expectancy. Although interplay between key CF plays a major role during disease progression, pathophysiology community in lungs remains poorly understood. Particular...
Epithelial cells are an important line of defense within the lung. Disruption epithelial barrier by pathogens enables systemic dissemination bacteria or viruses host leading to severe diseases with fatal outcomes. Thus, lung epithelium can be damaged seasonal and pandemic influenza A viruses. Influenza virus infection induced dysregulation immune system is beneficial for lower respiratory tract, causing bacterial viral co-infection. Host regulate protein homeostasis response different...
Abstract Phytoplankton blooms initiate bacterioplankton blooms, from which bacterial biomass is released via grazing zooplankton and viral lysis. Bacterial consumption of algal during well studied, but little known about the simultaneous reuse necromass. Alpha- beta-glucans are abundant dissolved organic macromolecules blooms. We demonstrate laminarin-fueled alpha-glucan synthesis in marine Bacteroidota strains, as these alpha-glucans major carbon source vitro a diatom-dominated bloom....
In Escherichia coli, the Twin-arginine translocation (Tat) pathway secretes a set of folded proteins with important physiological functions to periplasm and outer membrane. The loss Tat secretion impairs membrane integrity leads decreased cell growth. Only recently, has gained more attention due its essential role in bacterial virulence applications production fully heterologous proteins. this study, we investigated influence deletion all active components on E. coli cells. comprehensive...
The field of systems biology has been rapidly developing in the past decade. However, data produced by "omics" approaches is lagging behind requirements this field, especially when it comes to absolute abundances membrane proteins. In present study, a novel approach for large-scale quantification challenging subset proteins established and evaluated using osmotic stress management Gram-positive model bacterium Bacillus subtilis as proof-of-principle precedent. Selected were labeled SNAP-tag,...
Organelle-specific protein translocation systems are essential for organelle biogenesis and maintenance in eukaryotes but thought to be absent from prokaryotic organelles. Here, we demonstrate that MamF-like proteins crucial the formation functionality of bacterial magnetosome Deletion mamF-like genes Alphaproteobacterium Magnetospirillum gryphiswaldense results severe defects positioning, biomineralization, magnetic navigation. These phenotypic result disrupted targeting a subset...
Tissue sections, which are widely used in research and diagnostic laboratories have already been examined by immunohistochemistry (IHC), may subsequently provide a resource for proteomic studies, even though only small amount of protein is available. Therefore, we established workflow tandem mass spectrometry-based profiling IHC specimens characterized defined brain area sections. We investigated the CA1 region hippocampus dissected from slices adult C57BL/6J mice. The contains detailed...
In proteomics, fast, efficient and highly reproducible sample preparation is of utmost importance, particularly in view fast scanning mass spectrometers enabling analyses large series. To address this need, we have developed the web application MassSpecPreppy that operates on open science OT-2 liquid handling robot from Opentrons. This platform can prepare up to 96 samples at once, performing tasks like BCA protein concentration determination, digestion with normalization,...
Abstract Organelle-specific protein translocation systems are essential for organelle biogenesis and maintenance in eukaryotes but thought to be absent from prokaryotic organelles. Here, we identified that MamF-like proteins involved the formation of bacterial magnetosome organelles share an ancient origin with Tic20 translocases found chloroplasts. Deletion mamF -like genes alphaproteobacterium Magnetospirillum gryphiswaldense results severe defects positioning, biomineralization, magnetic...