A5000744299- Advanced Proteomics Techniques and Applications
- RNA Interference and Gene Delivery
- Antimicrobial Peptides and Activities
- Chromosomal and Genetic Variations
- Salivary Gland Disorders and Functions
- Mass Spectrometry Techniques and Applications
- Bacterial Infections and Vaccines
- Antimicrobial Resistance in Staphylococcus
- Circular RNAs in diseases
- Bacterial Genetics and Biotechnology
- Oral microbiology and periodontitis research
- Cardiac Fibrosis and Remodeling
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Advanced biosensing and bioanalysis techniques
- Enzyme Structure and Function
- Marine and coastal ecosystems
- Biosensors and Analytical Detection
- Sperm and Testicular Function
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- Biochemical and Structural Characterization
- Bacterial biofilms and quorum sensing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- 14-3-3 protein interactions
Universitätsmedizin Greifswald
2014-2023
German Centre for Cardiovascular Research
2020-2022
Centre for Cellular and Molecular Biology
1996-2021
Universität Greifswald
2010-2020
Bethany College - West Virginia
2018
Indian Institute of Chemical Technology
2017
University of Michigan
2006-2014
Center for Systems Biology
2013
Digital Proteomics (United States)
2013
National Institute of Oceanography
1988
Tumor cells secrete not only a variety of soluble factors, but also extracellular vesicles that are known to support the establishment favorable tumor niche by influencing surrounding stroma cells.Here we show tumor-derived microvesicles (T-MV) directly influence enhancing their invasion in both autologous and heterologous manner.Neither respective vesicle-free supernatant nor MV from benign mammary mediate invasion.Uptake T-MV is essential for proinvasive effect.We further identify highly...
Abstract Aim Interest in human saliva proteomics for disease‐specific biomarker screening increased the last decade. We used whole samples from periodontally healthy and diseased subjects with chronic periodontitis to screen disease‐associated differences protein pattern. Material Methods selected 20 population‐based cross‐sectional Study of Health Pomerania ( SHIP ‐2 ‐Trend). Saliva collection was performed commercially available Salivette ® (Sarstedt, Nümbrecht, Germany). Whole proteins...
Ischemia-reperfusion injury (IRI) is the leading cause of ARF. A pathophysiologic role coagulation system in renal IRI has been established, but functional relevance thrombomodulin (TM)-dependent activated protein C (aPC) generation and intracellular targets aPC remain undefined. Here, we investigated TM-dependent therapeutic application a murine model an vitro hypoxia reoxygenation (HR) using proximal tubular cells. In IRI, endogenous levels were reduced. Genetic or reconstitution...
By ubiquitylating a protein interaction domain in Dishevelled, Huwe1 acts as negative feedback regulator of Wnt signaling.
Abstract Epidemiological studies found an association between increased platelet size and the risk for thrombotic complications, but functional differences of large small platelets remain poorly understood due to a lack standardized protocols separating with different size. We designed protocol separate from 15 mL whole blood. Separated fractions differed in mean volume: 12.1 fl (10.3–13.8 fl) versus 7.7 (6.8–9.5 fl, p < 0.01), forward scatter fluorescence intensity: 24.75 (19.9–30.9)...
Abstract Gliomas are the most common of primary intracranial tumors with astrocytomas constituting about 40%. Using clinically and histologically assessed astrocytomas, we have studied their protein profiles using a two‐dimensional gel electrophoresis‐mass spectrometry approach identified differentially expressed proteins which may be useful molecular indicators to understand these tumors. Examination 27 astrocytoma samples different grades revealed 72 distinct, belonging various functional...
Growth factor receptor mediated signaling is meanwhile recognized as a complex network, which initiated by recruiting specific patterns of adaptor proteins to the intracellular domain epidermal growth (EGFR). Approaches globally identify EGFR-binding are required elucidate this network. We affinity-purified EGFR with its interacting coprecipitation from lysates A431 cells. A total 183 were repeatedly detected in high-resolution MS measurements. For 15 these, direct interactions listed...
Capacitation confers on the spermatozoa competence to fertilize oocyte. At molecular level, a cyclic adenosine monophosphate (cAMP) dependent protein tyrosine phosphorylation pathway operates in capacitated spermatozoa, thus resulting of specific proteins. Identification these tyrosine-phosphorylated proteins and their function with respect hyperactivation acrosome reaction, would unravel basis capacitation. With this view, 21 phosphotyrosine have been identified hamster out which 11 did not...
Staphylococcus aureus is a versatile gram-positive pathogen that gains increasing importance due to the rapid spreading of resistances. Functional genomics technologies can provide new insights into adaptational network this bacterium and its response environmental challenges. While functional technologies, including proteomics, have been extensively used study these phenomena in shake flask cultures, studies bacteria from vivo settings lack behind. Particularly for proteomics studies, major...
Background and Objective Interest in human saliva is increasing for disease‐specific biomarker discovery studies. However, protein composition of whole can grossly vary with physiological environmental factors over time it comprises as well bacterial proteins. Material Methods We compared intra‐ inter‐subject variabilities using complementary gel‐based (two‐dimensional difference gel electrophoresis, 2‐D DIGE ) gel‐free (liquid chromatography tandem mass spectrometry, LC ‐ MS / proteomics...
Staphylococcus aureus is an opportunistic pathogen that can cause life-threatening infections, particularly in immunocompromised individuals. The high-level virulence of S. largely relies on its diverse and variable collection factors immune evasion proteins, including the six serine protease-like proteins SplA to SplF. Spl are expressed by most clinical isolates aureus, but little known about molecular mechanisms which these modify host's response for benefit bacteria. Here, we identify...
Streptococcus pneumoniae (pneumococci) is a leading cause of severe bacterial meningitis in many countries worldwide. To characterize the repertoire fitness and virulence factors predominantly expressed during we performed niche-specific analysis vivo proteome mouse model, which bacteria are directly inoculated into cerebrospinal fluid (CSF) cisterna magna. We generated comprehensive mass spectrometry (MS) spectra library enabling even presence eukaryotic proteins. recovered 200,000...
Abstract Stressosomes are stress-sensing protein complexes widely conserved among bacteria. Although a role in the regulation of general stress response is well documented Gram-positive bacteria, activating signals still unclear, and little known about physiological function stressosomes Gram-negative Here we investigated stressosome marine pathogen Vibrio vulnificus . We demonstrate that it senses oxygen identified its modulating iron-metabolism. determined cryo-electron microscopy...
Hypusine modification of eukaryotic initiation factor 5A (eIF-5A) represents a unique and highly specific post-translational with regulatory functions in cancer, diabetes, infectious diseases. However, the cellular pathways that are influenced by hypusine remain largely unknown. To globally characterize eIF-5A hypusine-dependent pathways, we used an approach combines large-scale bioreactor cell culture tandem affinity purification mass spectrometry: "bioreactor-TAP-MS/MS." By applying this...
Whole saliva is gaining more and attention as a diagnostic tool to study disease-specific changes in human subjects. Prior the actual disease-related analyses, it important understand influence of various demographic variables coupled phenotypes on salivary protein signatures. In cross-sectional approach, we analyzed age, sex, body mass index (BMI), smoking, education signatures whole samples 187 individuals. Subjects were randomly selected from population-based Study Health Pomerania...