A5081020049- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Immune Response and Inflammation
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Probiotics and Fermented Foods
- Enzyme Structure and Function
- RNA modifications and cancer
- Invertebrate Immune Response Mechanisms
- Alzheimer's disease research and treatments
- Aquaculture disease management and microbiota
- Polydiacetylene-based materials and applications
- Genomics and Chromatin Dynamics
- Insect symbiosis and bacterial influences
- Bacteriophages and microbial interactions
- Click Chemistry and Applications
- Cancer Research and Treatments
- Chemical Reactions and Isotopes
- Signaling Pathways in Disease
- Advanced Glycation End Products research
- Escherichia coli research studies
- Glycosylation and Glycoproteins Research
- Computational Drug Discovery Methods
- Neuropeptides and Animal Physiology
Institute of Life Sciences
2019-2022
Bose Institute
2013-2019
Abstract The recent increase in multidrug resistance against bacterial infections has become a major concern to human health and global food security. Synthetic antimicrobial peptides (AMPs) have recently received substantial attention as potential alternatives conventional antibiotics because of their potent broad-spectrum activity. These also been implicated plant disease control for replacing treatment methods that are polluting hazardous the environment health. Here, we report de novo...
A non-toxic, nine residue peptide, NIVNVSLVK is shown to interfere with insulin fibrillation by various biophysical methods. Insulin undergoes conformational changes under certain stress conditions leading amyloid fibrils. Fibrillation of poses a problem in its long-term storage, reducing efficacy treating type II diabetes. The dissociation oligomer monomer the key step for onset fibrillation. time course at 62°C using Thioflavin T fluorescence shows an increase lag from 120 min without...
Abstract
KYE28 (KYEITTIHNLFRKLTHRLFRRNFGYT-LR), the representative sequence of helix D heparin co-factor II, was demonstrated to be potent against agronomically important Gram-negative plant pathogens Xanthomonas vesicatoria and oryzae, capable inhibiting disease symptoms in detached tomato leaves. NMR studies presence lipopolysaccharide provided structural insights into mechanisms underlying this, notably relationship outer membrane permeabilization. The three-dimensional solution structure LPS is...
A designed nontoxic, nonhemolytic 11-residue peptide, NF11 (NAVRWSLMRPF), not only inhibits the aggregation of amyloid beta (Aβ40) protein but also disaggregates preformed oligomers and mature Aβ fibrils, thereby reducing associated-toxicity. NMR experiments provide evidence NF11's ability to inhibit fibril formation, primarily through interaction with N-terminus region as well central hydrophobic cluster Aβ40. has micromolar binding affinity toward both monomeric aggregated species for...
Mechanistic insights into the permeabilization of outer membrane Gram negative bacteria by an antimicrobial peptide lactoferrampin, a 17 residue peptide, using high and low resolution spectroscopy in conjunction with MD simulation.
The objective of the present study is investigation possibilities for boosting peptide anti-inflammatory effects by tryptophan end-tagging, including identification underlying mechanisms this. In doing so, end-tagging KYE21 (KYEITTIHNLFRKLTHRLFRR), a derived from heparin co-factor II, on membrane and lipopolysaccharide (LPS) interactions were investigated ellipsometry, NMR, fluorescence spectroscopy, circular dichroism measurements. Through its N-terminal W stretch, WWWKYE21 displays higher...
Successful use of microgels as delivery systems antimicrobial peptides (AMPs) requires control factors determining peptide loading and release to/from the well membrane interactions both microgel particles released peptides. Addressing these, we here investigate effects charge density conformationally induced amphiphilicity on AMP using detailed nuclear magnetic resonance (NMR) structural studies combined with ellipsometry, isothermal titration calorimetry, circular dichroism, light...
In this study, we report an interaction study of a 13-residue analogue peptide VG13P (VARGWGRKCPLFG), derived from designed VG16KRKP (VARGWKRKCPLFGKGG), with Lys6Gly mutation and removal the last three residues Lys14-Gly15-Gly16, in lipopolysaccharide (LPS), major component outer membrane Gram-negative bacteria responsible for sepsis or septic shock. displays enhanced anti-endotoxin property as evident significant reduction LPS-induced TNF-α gene expression levels monocytic cell line, while...
Abstract FKBP53 is one of the seven multi-domain FK506-binding proteins present in Arabidopsis thaliana, and it known to get targeted nucleus. It has a conserved PPIase domain at C-terminus highly charged N-terminal stretch, which been reported bind histone H3 perform function chaperone. To better understand molecular details this with chaperoning activity, we have solved crystal structures its terminal domains functionally characterized them. The C-terminal showed strong no role revealed...
Histone proteins associate with DNA to form the eukaryotic chromatin. The basic unit of chromatin is a nucleosome, made up histone octamer consisting two copies core histones H2A, H2B, H3, and H4, wrapped around by DNA. composed an H2A/H2B dimer single copy H3/H4 tetramer. highly charged are prone non-specific interactions several in cellular cytoplasm nucleus. chaperones diverse class that shuttle from into nucleus aid their deposition onto DNA, thus assisting nucleosome assembly process....
Abstract White spot syndrome virus (WSSV) causes severe disease in crustaceans, specifically shrimp, leading to substantial economic losses the aquaculture industry. Existing treatment methods fail sufficiently curb occurrence and spread of disease, necessitating development effective control strategies. Structural studies have primarily focused on envelope proteins a few non‐structural involved host entry, viral replication, assembly immune evasion, thus presenting themselves as potential...