A5014595517- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Seaweed-derived Bioactive Compounds
- Antifungal resistance and susceptibility
- Echinoderm biology and ecology
- Marine and coastal plant biology
- Microbial Metabolites in Food Biotechnology
- Polysaccharides and Plant Cell Walls
- Pneumonia and Respiratory Infections
- Chemical Synthesis and Analysis
- Enzyme Production and Characterization
- Proteoglycans and glycosaminoglycans research
- Pneumocystis jirovecii pneumonia detection and treatment
- Fungal Infections and Studies
- Monoclonal and Polyclonal Antibodies Research
- Microbial Natural Products and Biosynthesis
- Galectins and Cancer Biology
- Natural product bioactivities and synthesis
- Probiotics and Fermented Foods
- Enzyme Catalysis and Immobilization
- Biological Activity of Diterpenoids and Biflavonoids
- Polyamine Metabolism and Applications
- Bacteriophages and microbial interactions
- Marine Sponges and Natural Products
- Legume Nitrogen Fixing Symbiosis
N.D. Zelinsky Institute of Organic Chemistry
2016-2025
Russian Academy of Sciences
2008-2021
Gause Institute of New Antibiotics Russian Academy of Medical Sciences
2021
Indian Institute of Science Education and Research Pune
2015
Laboratoire de Chimie Organique
2013
Centre National de la Recherche Scientifique
2013
Centre de Recherches sur les Macromolécules Végétales
2013
Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
2013
Université Claude Bernard Lyon 1
2013
Biolitec (Germany)
2001-2012
The anti-inflammatory, antiangiogenic, anticoagulant, and antiadhesive properties of fucoidans obtained from nine species brown algae were studied in order to examine the influence fucoidan origin composition on their biological activities. All inhibited leucocyte recruitment an inflammation model rats, neither content fucose sulfate nor other structural features polysaccharide backbones significantly affected efficacy this model. In vitro evaluation P-selectin-mediated neutrophil adhesion...
Significance Poly- N -acetylglucosamine (PNAG) has been identified as a conserved surface polysaccharide produced by major bacterial, fungal, and protozoal parasites, including malarial sporozoites blood-stage forms, which can all be targeted for vaccination using this single antigen. Surface carbohydrates are among the most successful vaccines against human microbial pathogens but have tremendous variability that complicates vaccine development. The species of bacteria, fungi, protozoa...
Sulfated polysaccharides from Laminaria saccharina (new name: Saccharina latissima) brown seaweed show promising activity for the treatment of inflammation, thrombosis, and cancer; yet molecular mechanisms underlying these properties remain poorly understood. The aim this work was to characterize, using in vitro vivo strategies, anti-inflammatory, anti-coagulant, anti-angiogenic, anti-tumor activities two main sulfated polysaccharide fractions obtained L. saccharina: a) L.s.-1.0 fraction...
The epidermal growth factor repeats of the Notch receptor are extensively glycosylated with three different O-glycans. O-Fucosylation and elongation by glycosyltransferase Fringe have been well studied shown to be essential for proper signaling. In contrast, biosynthesis O-glucose O-N-acetylglucosamine is less understood. Recently, isolation Drosophila mutant rumi has that absence impairs function. O-Glucose further extended two contiguous α1,3-linked xylose residues. We identified enzymes...
Galectins, a family of structurally related carbohydrate-binding proteins, contribute to different events associated with cancer biology, including apoptosis, homotypic cell aggregation, angiogenesis and tumor-immune escape. To interfere galectin–carbohydrate interactions during tumor progression, current challenge is the design specific galectin inhibitors for therapeutic purposes. Here, we report synthesis three novel low molecular weight synthetic lactulose amines (SLA): (1)...
Vaccines for pathogens usually target strain-specific surface antigens or toxins, and rarely is there broad antigenic specificity extending across multiple species. Protective antibodies bacteria are specific capsular antigens. beta-(1-->6)-Poly-N-acetyl-d-glucosamine (PNAG) a polysaccharide produced by many pathogens, including Staphylococcus aureus, Escherichia coli, Yersinia pestis, Bordetella pertussis, Acinetobacter baumannii, others. to PNAG elicited when deacetylated glycoform...
The human natural killer cell carbohydrate, HNK-1, plays function-conducive roles in peripheral nerve regeneration and synaptic plasticity. It is also the target of autoantibodies polyneuropathies. thus important to synthesize structurally related HNK-1 carbohydrates for optimizing its roles, diagnosis neutralization fatal Guillain–Barré syndrome. As a first step toward these goals, we have synthesized several carbohydrate derivatives assess specificity monoclonal antibodies from rodents:...
The extracellular domain of Notch contains epidermal growth factor (EGF) repeats that are extensively modified with different O-linked glycans. O-Fucosylation is essential for receptor function, and elongation N-acetylglucosamine, catalyzed by members the Fringe family, modulates activity. Only recently, genes encoding enzymes involved in O-glucosylation pathway have been cloned. In Drosophila mutant rumi, characterized a mutation protein O-glucosyltransferase, signaling impaired...
Three structurally different fucoidans from the brown seaweeds Saccharina latissima (SL), Fucus vesiculosus (FV), and Cladosiphon okamuranus (CO), two chemically modified with a higher degree of sulfation (SL-S, CO-S), synthetic totally sulfated octasaccharide (OS), related to fucoidans, were assessed on anticoagulant antithrombotic activities in vitro experiments. The effects shown depend structural features compounds tested. Native fucoidan SL (DS) 1.3 was found be most active sample, FV...
β-(1→3)-D-Glucan is an essential component of the fungal cell wall. Mouse monoclonal antibodies (mAbs) against synthetic nona-β-(1→3)-D-glucoside conjugated with bovine serum albumin (BSA) were generated using hybridoma technology. The affinity constants two selected mAbs, 3G11 and 5H5, measured by a surface plasmon resonance biosensor assay biotinylated nona-β-(1→3)-D-glucan as ligand, approximately 11 nM 1.9 nM, respectively. glycoarray, which included series oligosaccharide derivatives...
Preparations of sulfated polysaccharides obtained from brown algae are known as fucoidans. These biopolymers have attracted considerable attention due to many biological activities which may find practical applications. Two Atlantic representatives Phaeophyceae, namely, Fucus vesiculosus and Ascophyllum nodosum, belonging the same order Fucales, popular sources commercial fucoidans, often regarded very similar in chemical composition actions. Nevertheless, these two fucoidan preparations...
Lysozyme is a well-known enzyme found in many biological fluids which plays an important role the antibacterial protection of humans and animals. assays are used for diagnosis number diseases utilized immunohistochemistry, genetic cellular engineering studies. The assaying methods divided into two categories measuring either concentration lysozyme as protein or its activity enzyme. While first category traditionally uses enzyme-linked immunosorbent assay (ELISA), determination enzymatic use...
Brucellosis is a dangerous zoonotic disease caused by bacteria of the genus
3-Aminopropyl α-(1→3)-pentaglucoside, a fragment of α-(1→3)-glucan the cell wall Aspergillus fumigatus, has been synthesized in blockwise approach. The application mono- and disaccharide N-phenyltrifluoroacetimidates bearing stereodirecting 6-O-benzoyl group was essential for stereoselective α-glucosylations. In products, p-methoxyphenyl levulinoyl groups served as orthogonal protecting anomeric position 3-OH group, respectively. Their removal from shared blocks led to donors acceptors that...