A5091271859- Mosquito-borne diseases and control
- Vector-borne infectious diseases
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Antifungal resistance and susceptibility
- Glycosylation and Glycoproteins Research
- Toxin Mechanisms and Immunotoxins
- Polysaccharides and Plant Cell Walls
- SARS-CoV-2 detection and testing
- Sex and Gender in Healthcare
- Carbohydrate Chemistry and Synthesis
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Immunotherapy and Immune Responses
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
Siberian Branch of the Russian Academy of Sciences
2018-2023
Institute of Chemical Biology and Fundamental Medicine
2018-2023
Novosibirsk State University
2018-2019
Volgograd State Medical University
1982
β-(1→3)-D-Glucan is an essential component of the fungal cell wall. Mouse monoclonal antibodies (mAbs) against synthetic nona-β-(1→3)-D-glucoside conjugated with bovine serum albumin (BSA) were generated using hybridoma technology. The affinity constants two selected mAbs, 3G11 and 5H5, measured by a surface plasmon resonance biosensor assay biotinylated nona-β-(1→3)-D-glucan as ligand, approximately 11 nM 1.9 nM, respectively. glycoarray, which included series oligosaccharide derivatives...
A panel of specific monoclonal antibodies (mAbs) against synthetic pentasaccharide β-D-Galf-(1→5)-[β-D-Galf-(1→5)]3-α-D-Manp, structurally related to Aspergillus fumigatus galactomannan, was generated using mice immunized with pentasaccharide-BSA conjugate and by hybridoma technology. Two selected mAbs, 7B8 8G4, could bind the initial affinity constants approximately 5.3 nM 6.4 nM, respectively, based on surface plasmon resonance-based biosensor assay. The glycoarray, built from a series...
Vaccinia virus (VACV) is a promising oncolytic agent because it exhibits many characteristic features of an virus. However, its effectiveness limited by the strong antiviral immune response induced this One possible approach to overcome limitation develop deimmunized recombinant VACV. It known that VACV p35 major protein for B- and T-cell response. Despite relevance p35, epitope structure remains insufficiently studied. To determine neutralizing epitopes, panel variants was designed,...
Tick-borne encephalitis virus (TBEV) is the most important tick-transmitted pathogen. It belongs to Flaviviridae family and causes severe human neuroinfections. In this study, protective efficacy of chimeric antibody chFVN145 was examined in mice infected with strains belonging Far-Eastern, European, Siberian subtypes TBEV, showed clear therapeutic when it administered once one, two, or three days after infection. The independent TBEV strain used infect mice; however, survival rate dependent...
Antibody-dependent enhancement (ADE) has been shown previously for SARS-CoV-1, MERS-CoV, and SARS-CoV-2 infection in vitro. In this study, the first monoclonal antibody (mAb) that causes ADE a vivo model was identified. mAb RS2 against S-protein developed using hybridoma technology. demonstrated sub-nanomolar affinity ability to neutralize vitro with IC50 360 ng/mL. an animal of infection, dose-dependent protective efficacy revealed. However, post-exposure prophylaxis, administration led...
A drug for the prevention and therapy of tick-borne encephalitis virus is being developed on basis protective chimeric antibody ch14D5a. At same time, epitope recognized by this surface glycoprotein E has not been localized yet. The aim work was to identify domain E, which ch14D5a binds. As a result, four recombinant variants were generated using bacterial expression system: (1) rE protein containing domains D1, D2, D3 E; (2) rED1+2 D1 D2; (3) rED3_301 protein, (4) rED3_294 comprising hinge...
There is evidence that NS1 glycoprotein plays an important role in the pathology of tick-borne encephalitis (TBE) and NS1-specific antibodies are detected blood patients with TBE. In this study, TBEV protein was produced HEK 293 cells. Obtained showed immunological properties similar to native protein.
Orthoflavivirus encephalitidis, formerly tick-borne encephalitis virus (TBEV), belongs to the genus. TBEV is transmitted by tick bites and infection with can lead serious disorders of central nervous system. In this study, a new protective monoclonal mouse antibody (mAb) FVN-32, high binding activity glycoprotein E TBEV, was selected examined in post exposure prophylaxis model infection. BALB/c mice were injected mAb FVN-32 at doses 200 μg, 50 12.5 μg per one day after challenge. showed...
Antibodies against the receptor-binding domain of SARS-CoV-2 spike protein (RBD S-protein) contribute significantly to humoral immune response during coronavirus infection (COVID-19) and after vaccination. The main focus study epitope composition RBD is concentrated on epitopes recognized by viral neutralizing antibodies. role antibodies that bind but are unable neutralize virus in formation remains unclear. In this investigation, immunochemical properties two mouse monoclonal RS17 S11 were...
Tick-borne encephalitis virus (TBEV), belonging to the Flaviviridae family, is most significant pathogen transmitted by Ixodes ticks and causing one of severe human neuroinfections. In Russia, serum immunoglobulin produced from donor blood currently used for post-exposure prophylactic therapy tick-borne virus. However, it known that preparations obtained donated have certain disadvantages, therefore development novel post exposure prophylaxis required. To develop an alternative preparation,...
A comparative analysis of the effectiveness antianginal drugs (papaverine, persantine, intensain, nitrong, anaprilin) in combination with climacteric myocardial dystrophy women was carried out. The data obtained make it possible to recommend beta-blockers (anaprilin, obzidan) for treatment coronary artery disease against background pathological menopause wider use.