A5069049348- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Aquaculture disease management and microbiota
- Brucella: diagnosis, epidemiology, treatment
- Advanced biosensing and bioanalysis techniques
- Burkholderia infections and melioidosis
- Advanced Biosensing Techniques and Applications
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Invertebrate Immune Response Mechanisms
Lomonosov Moscow State University
2021-2025
N.D. Zelinsky Institute of Organic Chemistry
2024-2025
Lysozyme is a well-known enzyme found in many biological fluids which plays an important role the antibacterial protection of humans and animals. assays are used for diagnosis number diseases utilized immunohistochemistry, genetic cellular engineering studies. The assaying methods divided into two categories measuring either concentration lysozyme as protein or its activity enzyme. While first category traditionally uses enzyme-linked immunosorbent assay (ELISA), determination enzymatic use...
The storage duration and hatchability of eggs largely depend on the lysozyme content in egg whites; therefore, determining status is important for characterizing their quality. For first time, a fast accurate method active whites has been proposed to establish using fluorescence polarization assay synthetic chitooligosaccharide conjugates with fluorescent label without sample preparation. hens, black chukars, quails, ducks, geese, turkeys, peacocks, ostriches were studied. Samples from...
Brucellosis in animals is an infectious disease caused by bacteria of the genus Brucella. Known methods for diagnosing brucellosis face some challenges, due to difficulties isolating and standardizing natural antigen. In this work, we investigated possibility using fluorescence polarization assay (FPA) with synthetic glycoconjugate biosensing tracers detect antibodies against Brucella as a new methodology brucellosis. Based on received results, fluorescein-labeled trisaccharide tracer most...
This study describes the applicability of fluorescence polarization assay (FPA) based on use FITC-labeled oligosaccharide tracers defined structure for measurement active lysozyme in hen egg white. Depending chain length tracer, this method detects both formation enzyme-to-tracer complex (because lectin-like, i.e., carbohydrate-binding action lysozyme) and tracer splitting chitinase activity lysozyme). Evaluation dynamics enables simultaneous lectin activities lysozyme, which is crucial its...