A5100327672- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Monoclonal and Polyclonal Antibodies Research
- MicroRNA in disease regulation
- Toxoplasma gondii Research Studies
- Cytomegalovirus and herpesvirus research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Circular RNAs in diseases
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- RNA Research and Splicing
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- Cancer-related molecular mechanisms research
- Metabolomics and Mass Spectrometry Studies
- Single-cell and spatial transcriptomics
- Bone Metabolism and Diseases
- Extracellular vesicles in disease
- Heme Oxygenase-1 and Carbon Monoxide
- Peptidase Inhibition and Analysis
- Enzyme Catalysis and Immobilization
Icahn School of Medicine at Mount Sinai
2020-2025
First Affiliated Hospital of Anhui Medical University
2015-2025
Anhui Medical University
2013-2025
Xinjiang Academy of Agricultural Sciences
2016-2024
Shenyang The Fourth Hospital of People
2024
The Fourth People's Hospital
2024
Institute of Quality Standards and Testing Technology for Agro Products
2016-2024
First Affiliated Hospital of Xi'an Jiaotong University
2016-2024
Fudan University
2021-2024
Zhongshan Hospital
2021-2024
PROTACs (proteolysis targeting chimeras) are an emerging class of promising therapeutic modalities that degrade intracellular protein targets by hijacking the cellular ubiquitin–proteasome system. However, potential toxicity in normal cells due to off-tissue on-target degradation effect limits their clinical applications. Precise control a PROTAC's activity tissue-selective manner could minimize toxicity/side-effects. To this end, we developed cancer cell selective delivery strategy for...
Transcription factors (TFs) represent a major class of therapeutic targets for the treatment human diseases including cancer. Although biological functions and even crystal structures many TFs have been clearly elucidated, there is still no viable approach to target majority TFs, thus rendering them undruggable decades. PROTACs (proteolysis targeting chimeras) emerge as powerful modalities, which rely on induced protein–protein interactions between proteins interest (POIs) E3 ubiquitin...
Background We explore sphingolipid-related genes (SRGs) in skin melanoma (SKCM) to develop a prognostic indicator for patient outcomes. Dysregulated lipid metabolism is linked aggressive behavior various cancers, including SKCM. However, the exact role and mechanism of sphingolipid remain partially understood. Methods integrated scRNA-seq data from patients sourced GEO database. Through utilization Seurat R package, we successfully identified distinct gene clusters associated with survival...
Proteolysis targeting chimeras (PROTACs) represent a new class of promising therapeutic modalities. PROTACs hijack E3 ligases and the ubiquitin-proteasome system (UPS), leading to selective degradation target proteins. However, only very limited number have been leveraged generate effective PROTACs. Herein, we report that KEAP1 ligase can be harnessed for targeted protein utilizing highly selective, noncovalent small-molecule binder. We generated proof-of-concept PROTAC, MS83, by linking...
Background and objectives: Ovarian cancer is the most fatal primary malignancy among gynecological cancers. Circular RNAs (circRNAs) play an important role in development of various cancers, but functions circRNAs ovarian have not been studied. We aim to explore function mechanism CDR1as progression provide a new target for diagnosis treatment cancer. Methods: cell proliferation was assessed according proliferating nuclear antigen (PCNA) Ki67 protein expression levels MTT CCK8 assays. The...
Molecular glues and proteolysis targeting chimeras (PROTACs) are promising new therapeutic modalities. However, the lack of specificity for molecular glue- or PROTAC-mediated in cancer cells versus normal raises potential toxicity concerns that will likely limit their clinical applications. Here, we developed a general strategy to deliver immunomodulatory imide drug (IMiD)-based PROTACs folate receptor α (FOLR1)-positive cells. Specifically, designed folate-caged pomalidomide prodrug,...
Targeted protein degradation approaches have been widely used for degrading oncogenic proteins, providing a potentially promising therapeutic strategy cancer treatment. However, to targeting tumor suppressor proteins are very limited, and only few agonists developed date. Here, we report the development of platform termed TF-DUBTAC, which links DNA oligonucleotide covalent ligand deubiquitinase OTUB1 via click reaction, selectively stabilize transcription factors. We three series TF-DUBTACs,...
Given the prevalent advancements in DNA- and RNA-based PROTACs, there remains a significant need for exploration expansion of more specific DNA-based tools, thus broadening scope repertoire PROTACs. Unlike conventional A- or B-form DNA, Z-form DNA is configuration that exclusively manifests itself under stress conditions with target sequences, which can be recognized by reader proteins, such as ADAR1 ZBP1, to exert downstream biological functions. The core our innovation lies strategic...
Toxoplasma gondii is an intracellular protozoan that affects most species of endothermic animals including humans with a great infection rate. The vertical transmission T. causes abortion, constituting serious threat to and leading losses in livestock production. Distinct from population structure North America Europe, Chinese 1 (ToxoDB #9) dominant genotype prevalent China. Among the isolates 1, Wh3 Wh6 have different virulence pathogenicity mice. However, little has been known about their...
Abnormal DNA methylation/demethylation is recognized as a hallmark of cancer. TET (ten-eleven translocation) family members are novel demethylation related proteins that dysregulate in multiple malignances. However, their effects on ovarian cancer remain to be elucidated. The changes during TGF-β1-induced epithelial-mesenchymal transition (EMT) SKOV3 and 3AO cells were detected. TET3 was ectopically expressed TGF-β1-treated examine its effect EMT phenotype. downstream target further...
The self-calibration capability of ratiometric signals has been widely considered to enhance the accuracy, sensitivity, and anti-interference ability immunoassays. Exploring a new approach generate can provide more options for various requirements. Herein, we integrated negative-readout competitive positive-readout noncompetitive immunoassays into single assay by employing different color tracers, labeled peptidomimetic anti-immunocomplex peptides, create unconstrained signal approach. Using...
Telomeres are naturally shortened after each round of cell division in noncancerous normal cells, while the activation telomerase activity to extend telomere cancer is essential for transformation. Therefore, telomeres regarded as a potential anticancer target. In this study, we report development nucleotide-based proteolysis-targeting chimera (PROTAC) designed degrade TRF1/2 (telomeric repeat-binding factor 1/2), which key components shelterin complex (telosome) that regulates length by...
Abstract: Arsenic trioxide (As 2 O 3 ) achieved dramatic remissions in patients with acute promyelocytic leukaemia. Clinical reports have shown that treatment was associated cardiotoxicity. We investigated the toxic mechanisms of As H9c2 cardiomyocytes. Clinically relevant concentrations (2–10 µM) reduced viability cells a concentration‐dependent manner. The decreased cell because induced apoptosis (cell shrinkage, nuclear alterations and caspase‐3 activation), or even necrosis at higher...
Milk is an important food component in the human diet and a target for fraud, including many unsafe practices. For example, unscrupulous adulteration of soymilk into bovine goat milk or order to gain profit without declaration health risk, as adulterant source sanitary history are unknown. A robust fit-for-purpose technique required enforce market surveillance hence protect consumer health. Nuclear magnetic resonance (NMR) powerful characterization products based on measuring profile...
Rheumatoid arthritis (RA) is characterized by a tumor-like expansion of the synovium and subsequent destruction adjacent articular cartilage bone. In our previous work we showed that phosphatase tension homolog deleted on chromosome 10 ( PTEN ) contributes to activation fibroblast-like synoviocytes (FLS) in adjuvant-induced (AIA), but underlying mechanism not unknown. this study, show downregulated while DNA methyltransferase (DNMT)1 upregulated FLS from RA patients rat model AIA....
Circular RNAs (circRNAs) have emerged as significant regulators in human cancers. We aimed to explore the functional role of circular RNA RHOBTB3 (circRHOBTB3) ovarian cancer.
Traditional Chinese medicine (TCM) not only maintains the health of Asian people but also provides a great resource active natural products for modern drug development. Herein, we developed Database Constituents Absorbed into Blood and Metabolites TCM (DCABM-TCM), first database systematically collecting blood constituents prescriptions herbs, including prototypes metabolites experimentally detected in blood, together with corresponding detailed detection conditions through manual literature...
Abstract This article reviews the development and application of chemiluminescence (CL) published in literature between January 2004 October 2006, with regard to instrumentation, systems, applications, conclusions.
Abstract Despite the high efficacy and safety of arsenic trioxide (ATO) in treating acute promyelocytic leukemia (APL) eradicating APL leukemia-initiating cells (LICs), mechanism underlying its selective cytotoxicity remains elusive. We have recently demonstrated that undergo a novel cell death program, termed ETosis, through autophagy. However, role ETosis ATO-induced LIC eradication unclear. For this study, we evaluated effects ATO on contributions drug-induced to eradication. In NB4...