A5003479391- Blood Coagulation and Thrombosis Mechanisms
- Phagocytosis and Immune Regulation
- Platelet Disorders and Treatments
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Hemophilia Treatment and Research
- Erythrocyte Function and Pathophysiology
- Blood properties and coagulation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Retinoids in leukemia and cellular processes
- Acute Myeloid Leukemia Research
- Hemoglobinopathies and Related Disorders
- Inflammasome and immune disorders
- Extracellular vesicles in disease
- Acute Ischemic Stroke Management
- SARS-CoV-2 and COVID-19 Research
- Neurological Disorders and Treatments
- Venomous Animal Envenomation and Studies
- Thermal Regulation in Medicine
- Atrial Fibrillation Management and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Pancreatitis Pathology and Treatment
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Chronic Myeloid Leukemia Treatments
VA Boston Healthcare System
2015-2024
Harvard University
2013-2024
Brigham and Women's Hospital
2007-2020
Case Western Reserve University
2006
BackgroundThe role of neutrophil extracellular traps (NETs) in procoagulant activity (PCA) stroke patients caused by thromboembolic occlusion the internal carotid artery (ICA) remains unclear. Our objectives were to evaluate critical NETs induction hypercoagulability and identify functional significance during atherothrombosis.MethodsThe levels NETs, activated platelets (PLTs), PLT-derived microparticles (PMPs) detected plasma 55 35 healthy controls. NET formation thrombi analysed using...
Lactadherin is a phosphatidyl-L-serine (Ptd-L-Ser)-binding protein that decorates membranes of milk fat globules. The major Ptd-l-Ser binding function lactadherin has been localized to its C2 domain, which shares homology with the domains blood coagulation factor VIII and V. Correlating this homology, purified competes efficiently factors V for Ptd-L-Ser sites, functioning as potent anticoagulant. We have determined crystal structure domain (Lact-C2) at 1.7A resolution. bovine Lact-C2...
Abstract The mechanisms that eliminate activated platelets in inflammation-induced disseminated intravascular coagulation (DIC) micro-capillary circulation are poorly understood. This study explored an alternate pathway for platelet disposal mediated by endothelial cells (ECs) through phosphatidylserine (PS) and examined the effect of clearance on procoagulant activity (PCA) sepsis. Platelets septic patients demonstrated increased levels surface activation markers apoptotic vesicle...
The mechanisms contributing to an increased risk of thrombosis in uremia are complex and require clarification. There is scant morphological evidence membrane-dependent binding factor Xa (FXa) Va (FVa) on endothelial cells (EC) vitro. Our objectives were confirm that exposed phosphatidylserine (PS) microparticle (MP), EC, peripheral blood cell (PBC) has a prothrombotic role uremic patients provide visible PS-dependent prothrombinase assembly We found had more circulating MP (derived from PBC...
Abstract Despite the high efficacy and safety of arsenic trioxide (ATO) in treating acute promyelocytic leukemia (APL) eradicating APL leukemia-initiating cells (LICs), mechanism underlying its selective cytotoxicity remains elusive. We have recently demonstrated that undergo a novel cell death program, termed ETosis, through autophagy. However, role ETosis ATO-induced LIC eradication unclear. For this study, we evaluated effects ATO on contributions drug-induced to eradication. In NB4...
Acute promyelocytic leukemia (APL) cells exhibit disrupted regulation of cell death and differentiation, therefore the fate these leukemic is unclear. Here, we provide first evidence that a small percentage APL undergo novel pathway by releasing extracellular DNA traps (ETs) in untreated patients. Both NB4 stimulated with serum had nuclear budding vesicles filled chromatin leaked to space when membranes ruptured. Using immunofluorescence, found undergoing ETosis extruded lattice-like...
Factor VIII functions as a cofactor for IXa in membrane-bound enzyme complex. Membrane binding accelerates the activity of VIIIa–Factor complex approx. 100000-fold, and major phospholipid-binding motif is thought to be on C2 domain. In present study, we prepared an fVIII-C2 (Factor domain) construct from Escherichia coli, confirmed its structural integrity through three distinct monoclonal antibodies. Solution-phase assays, performed with flow cytometry FRET (fluorescence resonance energy...
Relatively little is known about the role of phosphatidylserine (PS) in procoagulant activity (PCA) patients with diabetic kidney disease (DKD). This study was designed to evaluate whether exposed PS on microparticles (MPs) and MP-origin cells were involved hypercoagulability DKD patients.DKD (n = 90) divided into three groups based urinary albumin excretion rate, defined as normoalbuminuria (No-A) (<30 mg/24 h), microalbuminuria (Mi-A) (30-299 h) or macroalbuminuria (Ma-A) (>300 compared...
Neoadjuvant chemotherapy is relevant to the formation of thromboembolism and secondary neoplasms in triple-negative breast cancer (TNBC). Chemotherapy-induced cell-derived microparticles (BCMPs) may have important thrombogenic pro-metastatic effects on platelets endothelium, which be related expression distribution phosphatidylserine (PS). However, investigating these interactions challenging due technical limitations.