A5100777538- Metabolism and Genetic Disorders
- Mitochondrial Function and Pathology
- Advanced biosensing and bioanalysis techniques
- Folate and B Vitamins Research
- RNA modifications and cancer
- Immunodeficiency and Autoimmune Disorders
- Ginseng Biological Effects and Applications
- Biosensors and Analytical Detection
- Biochemical and Molecular Research
- Advanced Biosensing Techniques and Applications
- Analytical chemistry methods development
- Diet and metabolism studies
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- Pluripotent Stem Cells Research
- Psoriasis: Treatment and Pathogenesis
- Blood disorders and treatments
- Skin Protection and Aging
- Pesticide Residue Analysis and Safety
- Glycosylation and Glycoproteins Research
- melanin and skin pigmentation
- ATP Synthase and ATPases Research
- Neonatal Health and Biochemistry
- Vascular Malformations and Hemangiomas
- Cancer-related molecular mechanisms research
Children's Hospital of Chongqing Medical University
2017-2025
Chongqing Medical University
2017-2025
People's Hospital of Xinjiang Uygur Autonomous Region
2012-2025
Beijing Children’s Hospital
2022-2025
Capital Medical University
2022-2025
First Affiliated Hospital of Zhengzhou University
2025
Gansu Agricultural University
2024
China Three Gorges University
2011-2024
Chongqing Public Health Medical Center
2024
University of Shanghai for Science and Technology
2024
The self-calibration capability of ratiometric signals has been widely considered to enhance the accuracy, sensitivity, and anti-interference ability immunoassays. Exploring a new approach generate can provide more options for various requirements. Herein, we integrated negative-readout competitive positive-readout noncompetitive immunoassays into single assay by employing different color tracers, labeled peptidomimetic anti-immunocomplex peptides, create unconstrained signal approach. Using...
The Wnt signaling pathway is abnormally activated in many human cancers. Secreted frizzled-related proteins (SFRPs) function as negative regulators of and play an important role carcinogenesis. SFRP promoter hypermethylation has often been identified cancers; however, the precise SFRPs cutaneous squamous cell carcinoma (SCC) unclear. methylation status family was analyzed age-and sex-matched case-control study, including 40 SCC cases normal controls, using MassARRAY EpiTYPER system. rate...
ATP-dependent chromatin-remodeling factors use the energy of ATP hydrolysis to alter structure chromatin and are important regulators eukaryotic gene expression. One such factor encoded by CHR5 (Chromatin-Remodeling Factor 5) in Arabidopsis (Arabidopsis thaliana) was previously found be involved regulation growth development. Here we show that is required for up-regulation intracellular immune receptor SNC1 (SUPPRESSOR OF npr1-1, CONSTITUTIVE1) consequently autoimmunity induced...
RAF, a core signaling component of the MAPK kinase cascade, is often mutated in various cancers, including melanoma, lung, and colorectal cancers. The approved inhibitors were focused on targeting BRAFV600E mutation that results constitutive activation through monomeric protein (Class I). However, these also paradoxically activate RAF dimers, resulting increased normal tissues. Recently, significant attention has turned to alterations dimeric (class II III BRAF NRAS). discovery potent...
T-cell receptor (TCR) diversity is crucial for adaptive immunity, yet baseline characterizations in pediatric populations remain sparse. We sequenced the TCRβ chain of 325 healthy Chinese children aged 0-18, categorized into six age groups. also analyzed cellular composition and associations using flow cytometry 81 these samples. Our results indicate a decrease with age, characterized by an increase high-frequency clonotypes notable changes CDR3 length V(D)J gene usage. These are influenced...
ABSTRACT Pediatric patients of autosomal dominant early onset osteoporosis conferred by heterozygous mutation in the WNT1 (OMIM: 615221) were rarely reported, and therapy pediatrics is relatively inexperienced. The clinical genotypic characteristics treatment process four children with caused monoallelic variation analyzed. admitted from June 2023 to January 2024. All presented multiple vertebral compression fracture, two them experienced recurrent peripheral fragility fractures. age first...
ABSTRACT Aims The objective of this study is to explore the regulatory role DNA methylation in delayed encephalopathy after carbon monoxide poisoning (DEACMP) and identify candidate epigenetic biomarkers. Methods In study, multi‐omics analyses such as methylomics, transcriptomics, pyrophosphate sequencing, qRT‐PCR, immunohistochemistry, western blotting were utilized investigate regulation altered gene expression pathogenesis DEACMP. Results Using integrated analysis, we identified 168...
Kaposi's sarcoma (KS) is a locally aggressive, multicentric tumor. RNA-binding proteins (RBPs) are pivotal for post-transcriptional regulation in various tumors. However, the aberrantly expressed RBP genes and their regulatory patterns KS remain unclear. This study aimed to identify relevant assess potential functions molecular interactions of RPS27, dysregulated tissues, Matched lesions normal control tissues from ten patients were chosen study. Differentially (DEGs) first identified by...
Oxidative stress plays a vital role in the pathogenesis of neurodegenerative diseases. Chikusetsu saponin V (CsV), most abundant member saponins from Panax japonicus (SPJ), has attracted increasing attention for its potential to treat However, mechanisms are unclear. Our study intended investigate antioxidative effects CsV human neuroblastoma SH-SY5Y cells. data showed that attenuated H2O2-induced cytotoxicity, inhibited ROS accumulation, increased activities superoxide dismutase (SOD) and...
The toxicity of 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is generally believed to be mediated by aryl hydrocarbon receptor (AhR), but some evidence suggests that the effects TCDD can also produced through AhR-independent mechanisms. In previous experiments, we found mainly AhR-dependent mechanism was involved in migration inhibition glioblastoma U87 cells TCDD. Due heterogeneity glioblastomas, not all tumor have significant AhR expression. and mechanisms on glioblastomas with low...
Phage borne peptides isolated from phage libraries have proven to be valuable reagents for the development of small-molecule immunoassays. However, large size, low diffusion rate, and biological nature particles create some limitations their use require secondary its detection. In this work, we explore Nano luciferase (NanoLuc) as a fusion partner generate recombinant tracers immunoassay development. The imidaclothiz peptidomimetic C2-15 that specifically binds anti-imidaclothiz monoclonal...
Objective: To analyz the current situation of diagnosis, treatment and prevention methylmalonic acidemia, phenotypes, biochemical features genotypes patients in mainland China, were investigated. Methods: Tottally 1 003 acidemia from 26 provinces municipalities China enrolled. The clinical data, gene mutations studied. Blood aminoacids acylcarnitines, urine organic acids, plasma total homocysteine determined for diagnosis. Gene analyses performed genetic study 661 patients. treated with...