A5059306082- Immunodeficiency and Autoimmune Disorders
- Immune Cell Function and Interaction
- interferon and immune responses
- RNA Research and Splicing
- RNA modifications and cancer
- Inflammasome and immune disorders
- Pediatric health and respiratory diseases
- T-cell and B-cell Immunology
- RNA regulation and disease
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Blood disorders and treatments
- NF-κB Signaling Pathways
- IL-33, ST2, and ILC Pathways
- Advanced Materials Characterization Techniques
- Genetics and Neurodevelopmental Disorders
- Blood groups and transfusion
- X-ray Diffraction in Crystallography
- Otitis Media and Relapsing Polychondritis
- Advanced Frequency and Time Standards
- Lymphoma Diagnosis and Treatment
- Biosensors and Analytical Detection
- Geophysics and Sensor Technology
- Toxoplasma gondii Research Studies
- Nuclear Structure and Function
Children's Hospital of Chongqing Medical University
2014-2025
Fudan University
2025
Chongqing Medical University
2014-2024
Ernst Ruska Centre
2023-2024
National Time Service Center
2023
Chinese Academy of Sciences
2023
Peking University
2017-2022
China International Science and Technology Cooperation
2022
Ministry of Education of the People's Republic of China
2016
Institute of Infection and Immunity
2016
mRNA m6A modification is heavily involved in modulation of immune responses. However, its function antiviral immunity controversial, and how responses regulate remains elusive. We here find TBK1, a key kinase pathways, phosphorylates the core methyltransferase METTL3 at serine 67. The phosphorylated interacts with translational complex, which required for enhancing protein translation, thus facilitating TBK1 also promotes activation to stabilize IRF3 mRNA. Type I interferon (IFN) induction...
Current molecular tests for tuberculosis (TB), such as whole genome sequencing and Xpert Mycobacterium tuberculosis/rifampicin resistance assay, exhibit limited sensitivity necessitate the pre-amplification step of target DNA. This limitation greatly increases detection time poses an increased risk infection. Here, we present a graphene field-effect transistor (GFET) based on CRISPR/Cas system detecting tuberculosis. The CRISPR/Cas12a has ability to specifically recognize cleave By...
Mitochondria not only serve as a platform for innate immune signaling transduction but also enhance responses by releasing mitochondrial DNA and RNA into the cytoplasm. However, whether matrix proteins could be liberated involved in remains enigmatic. Here, we identify protein ERA G-protein-like 1 (ERAL1) antiviral (MAVS)-interacting using proximity-based labeling technology. ERAL1 deficiency markedly reduces downstream triggered viruses. Moreover, ERAL1-deficient mice are more susceptible...
Deficiency in ELF4, X-linked (DEX) is a newly identified monogenic autoinflammatory disease. Most reported cases are male, leading to the recognition of DEX being primarily limited male patients. Here we described 3 pediatric female patients with from unrelated families, who all heterozygous for ELF4 mutations (c.320_c.321insA, c.329delA and c.685 A > G). Similar patients, main clinical features include recurring oral ulcers, abdominal pain diarrhea colonoscopy showing ulcers colon....
RNA and protein are interconnected biomolecules that can influence each other's life cycles functions through physical interactions. Abnormal RNA-protein interactions lead to cell dysfunctions human diseases. Therefore, mapping networks of is crucial for understanding cellular processes pathogenesis related Different practical protein-centric methods studying have been reported, but few robust RNA-centric exist. Here, we developed CRISPR-based proximity proteomics (CBRPP), a new method...
Ulcerative colitis (UC) is a chronic inflammatory bowel disease, characterized by relapsing and remitting colon mucosal inflammation. For patients suffering from UC, higher risk of cancer has been widely recognized. Here, we found that Elf4−/− mice developed tumors with 3 cycles dextran sulfate sodium salt (DSS) treatment alone. We further showed ELF4 suppression was prevalent in both UC DSS-induced models, this caused promoter region methylation. ELF4, upon PARylation PARP1,...
The innate immune system plays a critical role in the initial antiviral response. However, timing and duration of these responses must be tightly regulated during infection to ensure appropriate cell activation anti-viral defenses. Here we demonstrate that response, negative regulator miR-221 was also induced an ELF4-dependent manner. We further show ELF4 promotes expression through direct binding its promoter. Overexpression knockdown assay can negatively regulate IFNβ production time virus...
Abstract Background TYK2 deficiency is a rare primary immunodeficiency disease caused by loss‐of‐function mutations of gene, which initially proposed as subset hyper‐IgE syndrome (HIES). However, accumulating evidence suggests TYK2‐deficient patients do not necessarily present with HIES characteristics, indicating vacuum knowledge on the exact roles in human immune system. Method Pathogenic effects were confirmed qRT‐PCR, Western blot, and protein stability assays. The responses to cytokines...
Abstract RNA-protein interactions play essential roles in tuning gene expression at RNA level and modulating the function of proteins. Abnormal lead to cell dysfunction human diseases. Therefore, mapping networks is crucial for understanding cellular mechanism pathogenesis Different practical protein-centric methods studying has been reported, but few RNA-centric exist. Here, we developed CRISPR-based proximity proteomics (CBRPP), a new method identify proteins associated with target native...
E. fischeriana has long been used as a traditional Chinese medicine.Recent studies reported that some compounds of exhibited antimicrobial and immune enhance activity. Innate system is essential for the surveillance inner outer threats, initial host defense responses modulation. The role natural drug compounds, including fischeriana, in innate regulation largely unknown. Here we demonstrate compound Dpo involved antiviral signaling. genome wide RNA-seq analysis revealed synergized induction...
Abstract TYK2 deficiency is a rare Primary immunodeficiency disease caused by loss of function mutations gene, which initially proposed as subset Hyper IgE syndrome (HIES). However, accumulating evidence suggest deficient patients do not necessarily present with HIES characteristics, indicating vacuum knowledge on the exact roles in human immune system. Here we describe five more cases presenting or without hyper levels, atopy and distinct pathogen infection profile, are novel mutations....
Abstract Homozygous mutations in Ig-like domain of LMNA cause severe progeria. Unlike typical HGPS mediated by progerin due to LMNAWT/G608G mutation, it remains elusive how these homozygous We here found that patients with LMNAR527C/R527C mutation developed an atypical progeria autoimmune symptoms. Compare this led more inflammation patients. MSCs from exhibited overt and cellular senescence. Mechanistically, attenuated its binding DNA protein BAF, which aberrant aggregation Lamin A...
Abstract Monogenic autoinflammatory diseases (mAIDs) are a heterogeneous group of affecting primarily innate immunity, with various specific genetic causes. Genetic diagnosis mAIDs can assist in the patient’s management and therapy. However, large number sporadic familial cases remain genetically uncharacterized. Here, we described pediatric patient suffering from recurrent viral bacterial respiratory infection, refractory oral ulcer constipation, who was clinically diagnosed inborn errors...
Introduction: The immunodeficiency, centromeric instability, facial anomalies syndrome (ICF) is a rare autosomal recessive disease defined by developmental delay, and abnormalities. Centromeric instability results in chromosomal rearrangements genomic methylation due to defect. ICF1 2 are caused biallelic mutations the DNA methyltransferase 3B gene DNMT3B zinc finger BTB domain-containing 24 ZBTB24, respectively. Patients without immunodeficiency but only with dysmorphism intellectual deficit exist.