A5100776744- Respiratory viral infections research
- Parvovirus B19 Infection Studies
- Viral Infections and Vectors
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Mosquito-borne diseases and control
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- interferon and immune responses
- Inflammasome and immune disorders
- Cytomegalovirus and herpesvirus research
- Prenatal Screening and Diagnostics
- RNA regulation and disease
- RNA Research and Splicing
- Dermatological and COVID-19 studies
- Anesthesia and Neurotoxicity Research
- Animal Virus Infections Studies
- Hippo pathway signaling and YAP/TAZ
- CRISPR and Genetic Engineering
- Axon Guidance and Neuronal Signaling
- Sinusitis and nasal conditions
- Nanoplatforms for cancer theranostics
- Oral and Maxillofacial Pathology
- Head and Neck Surgical Oncology
- Vector-Borne Animal Diseases
Henan University
2019-2025
Anhui Agricultural University
2023
University of Kansas Medical Center
2014-2022
Chinese Academy of Medical Sciences & Peking Union Medical College
2019
Abstract Cardiovascular disease is the leading cause of death worldwide. Reperfusion therapy vital to patient survival after a heart attack but can myocardial ischemia/reperfusion injury (MI/RI). Nitric oxide (NO) ameliorate MI/RI and key molecule for drug development. However, reactive oxygen species (ROS) easily oxidize NO peroxynitrite, which causes secondary cardiomyocyte damage. Herein, L‐arginine‐loaded selenium‐coated gold nanocages (AAS) are designed, synthesized, modified with PCM...
Human parvovirus B19 (B19V) infection of human erythroid progenitor cells (EPCs) induces a DNA damage response and cell cycle arrest at late S phase, which facilitates viral replication. However, it is not clear exactly cellular factors are employed by this single-stranded virus. Here, we used microarrays to systematically analyze the dynamic transcriptome EPCs infected with B19V. We found that metabolism, replication, repair, response, cycle, pathways were significantly regulated after B19V...
Human bocavirus 1 (HBoV1) is a human parvovirus that causes acute respiratory tract infections in young children. In this study, we confirmed that, when polarized/well-differentiated airway epithelia are infected with HBoV1 vitro, they develop damage characterized by barrier function disruption and cell hypotrophy. Cell death mechanism analyses indicated the infection induced pyroptotic caspase-1 activation. Unlike other parvoviruses, did not activate apoptotic or necroptotic pathway. When...
Glioblastoma (GBM), a formidable and highly aggressive form of brain cancer, is predominantly driven by GBM stem cells (GSCs), which are characterized their ability for self-renewal aberrant differentiation. Targeting the terminal differentiation GSCs represents promising therapeutic strategy. This study aimed to elucidate role synapsin III (SYN3) in driving into neuron-like its effect on tumor-suppressive pathways GBM. Proliferation assays, limited dilution immunocytochemistry, western...
Human bocavirus type-1 (HBoV1) has a high tropism for the apical membrane of human airway epithelia. The packaging recombinant adeno-associated virus 2 (rAAV2) genome into HBoV1 capsid produces chimeric vector (rAAV2/HBoV1) that also efficiently transduces As such, this is attractive use in gene therapies to treat lung diseases such as cystic fibrosis. However, preclinical development rAAV2/HBoV1 vectors been hindered by fact humans are only known host infection. This study reports capable...
Human bocavirus 1 (HBoV1) is an autonomous parvovirus that infects well-differentiated primary human airway epithelia (HAE) in vitro In embryonic kidney HEK293 cells, the transfection of a duplex HBoV1 genome initiates viral DNA replication and produces progeny virions are infectious HAE. takes advantage signaling pathways damage response for efficient amplification both (nondividing) HAE dividing cells. On other hand, adeno-associated virus 2 (AAV2) helper-dependent dependoparvovirus,...
Human bocavirus 1 (HBoV1), a member of the genus Bocaparvovirus family Parvoviridae , causes acute respiratory tract infections in young children. Well-differentiated pseudostratified human airway epithelium cultured at an air-liquid interface (HAE-ALI) is ideal vitro culture model to study HBoV1 infection. Unique other parvoviruses, bocaparvoviruses express small nonstructured protein NP1 ~25 kDa from open reading frame (ORF) center viral genome. plays important role DNA replication and...
Pathogenic hantaviruses delay the type I interferon response during early stages of viral infection. However, robust and induction interferon-stimulated genes observed later hantavirus infection fail to combat virus replication in infected cells. Protein kinase R (PKR), a classical gene product, phosphorylates eukaryotic translation initiation factor eIF2α causes translational shutdown create roadblocks for synthesis proteins. The PKR-induced helps host cells establish an antiviral state...
ABSTRACT Viral ribonucleocapsids harboring the viral genomic RNA are used as template for mRNA synthesis and replication of genome by RNA-dependent polymerase (RdRp). Here we show that hantavirus nucleocapsid protein (N protein) interacts with RdRp in virus-infected cells. We mapped binding domain at N terminus protein. Similarly, pocket is located C RdRp. demonstrate an protein-RdRp interaction required function during course virus infection host cell.
Human bocavirus 1 (HBoV1), an autonomous parvovirus, is a helper virus supporting replication of wild-type adeno-associated 2 (AAV2). In this study, we compared the functions from HBoV1 with those adenovirus (Ad) for production recombinant AAV (rAAV) vector in HEK293 cells. We demonstrated that triple plasmids transfection (1) cloned minigenome (pBocaHelper) expresses genes NP1, NS2, and BocaSR, (2) pAAV transfer plasmid, (3) pAAVRepCap supports rAAV Despite yield 1-2 log lower than using...
Human parvovirus B19 (B19V), a member of the genus Erythroparvovirus family Parvoviridae, is small nonenveloped virus that has single-stranded DNA (ssDNA) genome 5.6 kb with two inverted terminal repeats (ITRs). B19V infection often results in severe hematological disorders and fetal death humans. replication follows model rolling hairpin-dependent replication, which large nonstructural protein NS1 introduces site-specific single-strand nick viral origins, locate at ITRs. executes...
ABSTRACT Human bocavirus 1 (HBoV1) belongs to the species Primate bocaparvovirus of genus Bocaparvovirus Parvoviridae family. HBoV1 causes acute respiratory tract infections in young children and has a selective tropism for apical surface well-differentiated human airway epithelia (HAE). In this study, we identified an additional gene, bocavirus-transcribed small noncoding RNA (BocaSR), within 3′ region (nucleotides [nt] 5199 5338) viral genome positive sense. BocaSR is transcribed by...
Human bocavirus 1 (HBoV1) is one of the significant pathogens causing acute respiratory tract infections in young children worldwide. HBoV1 encodes a small nonstructural protein (NP1) that plays an important role maturation viral mRNAs encoding capsid proteins as well DNA replication. Here, we identified critical host factor, CPSF6, directly interacts with NP1, mediates nuclear import and protein-encoding nucleus. The identification direct interaction between NP1 CPSF6 provides new insights...
Severe fever with thrombocytopenia syndrome (SFTS) virus, recently named Bandavirus dabieense, belongs to the genus of family Phenuiviridae, and it causes SFTS in humans clinical symptoms including fever, thrombocytopenia, gastrointestinal symptoms, leukocytopenia. However, there are few reports on pathogenesis SFTSV animals. This study first isolated strain SD22-2 from sick-farmed mink. Viral metagenomics was used detect nucleotide specimens obtained symptomatic minks. Then, we virus using...
Hantavirus nucleocapsid protein (NP) inhibits kinase R (PKR) dimerization by an unknown mechanism to counteract its antiviral responses during virus infection. Here we demonstrate that NP exploits endogenous PKR inhibitor P58 IPK inhibit PKR. The activity of is normally restricted in cells the formation inactive complex with negative regulator Hsp40. On other hand, remains associated 40S ribosomal subunit, a unique strategic location facilitates free access downstream target eIF2α. Although...
Adeno-associated virus (AAV) requires co-infection with helper for efficient replication. We previously reported that Human Bocavirus 1 (HBoV1) genes, including
Parvoviruses package a linear single-stranded DNA genome with hairpin structures at both ends. It has been thought that terminal sequences are indispensable for viral replication. Here, we provide evidence the hairpin-deleted duplex genomes of human bocavirus 1 (HBoV1) replicate in embryonic kidney 293 (HEK293) cells. We propose an alternative model HBoV1 replication which leading strand can initiate displacement without transfer. The transfection retain minimal origin right end (OriR) but...