A5026748866- Parvovirus B19 Infection Studies
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Respiratory viral infections research
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Dermatological and COVID-19 studies
- Prenatal Screening and Diagnostics
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Radioactive contamination and transfer
- Influenza Virus Research Studies
- Trace Elements in Health
- Virology and Viral Diseases
- Epigenetics and DNA Methylation
- Animal Disease Management and Epidemiology
- Erythrocyte Function and Pathophysiology
- Vector-Borne Animal Diseases
- COVID-19 Clinical Research Studies
- Immune responses and vaccinations
- CAR-T cell therapy research
- Cancer-related molecular mechanisms research
China Agricultural University
2014-2025
University of Kansas Medical Center
2019-2024
Shandong Provincial QianFoShan Hospital
2021
Shandong University
2021
Guangxi Center for Disease Prevention and Control
2016-2021
Adeno-associated viruses (AAVs) package a single-stranded (ss) DNA genome of 4.7 kb in their capsid ~20 nm diameter. AAV replication requires co-infection helper virus, such as adenovirus. During the optimization recombinant production, small viral nonstructural protein, membrane-associated accessory protein (MAAP), was identified. However, function MAAP context infection remains unknown. Here, we investigated expression strategy and during both AAV2 AAV5 human embryonic kidney (HEK)293...
Parvovirus B19 (B19V) infects human erythroid progenitor cells (EPCs) and causes several hematological disorders fetal hydrops. Amino acid (aa) 5-68 of minor capsid protein VP1 (VP1u
Human bocavirus 1 (HBoV1) is a human parvovirus that causes lower respiratory tract infections in young children. It contains single-stranded (ss) DNA genome of ~5.5 kb encodes small noncoding RNA 140 nucleotides known as bocavirus-encoded (BocaSR), addition to viral proteins. Here, we determined the secondary structure BocaSR vivo by using DMS-MaPseq. Our findings reveal undergoes N6-methyladenosine (m6A) modification at multiple sites, which critical for replication both dividing HEK293...
The pathogenicity of a variant goose parvovirus (GPV), isolated from short beak and dwarfism syndrome Pekin ducks (strain Cherry Valley), was investigated in embryonating eggs goslings. virus easily grown GPV antibody-free embryos caused high mortality severe lesions embryos, indicating that the has good adaptation to embryonated similar classical GPV. Like third egg-passage H) GPV, JS1) Derzsy's disease 2-day-old goslings with mortality. findings suggest strain, which had specifically...
Abstract In the early 2000s, emerging SARS‐CoV‐2, which is highly pathogenic, posed a great threat to public health. During COVID‐19, epigenetic regulation deemed be an important part of pathophysiology and illness severity. Using Illumina Infinium Methylation EPIC BeadChip (850 K), we investigated genome‐wide differences in DNA methylation between healthy subjects COVID‐19 patients with different disease severities. We conducted combined analysis selected 35 “marker” genes that could...
A novel virus was detected from diseased ducks and completely determined. The shown to have a picornavirus-like genome layout. Interestingly, the contained total of up six 2As, including four 2As (2A1-2A4) each having an NPGP motif, AIG1-like 2A5, parechovirus-like 2A6. 5'UTR predicted possess hepacivirus/pestivirus-like internal ribosome entry site (IRES). However, subdomain IIIe consisted 3 nt stem five unpaired bases, distinct those found in all other HP-like IRESs. most closely related...
During infection of human parvovirus B19 (B19V), one viral precursor mRNA (pre-mRNA) is transcribed by a single promoter and alternatively spliced polyadenylated. Here, we identified novel cis-acting sequence (5'-GUA AAG CUA CGG GAC GGU-3'), intronic splicing enhancer 3 (ISE3), which lies 72 nucleotides upstream the second splice acceptor (A2-2) site intron that defines exon encoding 11-kDa nonstructural protein. RNA binding motif protein 45 (RBM45) specifically binds to ISE3 with high...
Human bocavirus 1 (HBoV1) is one of the significant pathogens causing acute respiratory tract infections in young children worldwide. HBoV1 encodes a small nonstructural protein (NP1) that plays an important role maturation viral mRNAs encoding capsid proteins as well DNA replication. Here, we identified critical host factor, CPSF6, directly interacts with NP1, mediates nuclear import and protein-encoding nucleus. The identification direct interaction between NP1 CPSF6 provides new insights...
Parvovirus B19 (B19V) infection causes diseases in humans ranging from the mild erythema infectiosum to severe hematological disorders. The unique region of minor structural protein VP1 (VP1u) 227 amino acids harbors strong neutralizing epitopes which elicit dominant immune responses patients. Recent studies have shown that VP1u selectively binds and enters B19V permissive cells through an unknown cellular proteinaceous receptor. In present study, we demonstrated purified recombinant...
Bourbon virus (BRBV) was first isolated from a patient hospitalized at the University of Kansas Hospital in 2014. Since then, several deaths have been reported to be caused by BRBV infection Midwest and Southern United States. is tick-borne that widely carried lone star ticks. It belongs genus Thogotovirus Orthomyxoviridae family. Currently, there are no treatments or vaccines available for thogotovirus diseases. In this study, we reconstituted replicon reporter system, composed plasmids...
Bourbon virus (BRBV) was first isolated from a blood sample collected male patient living in County, Kansas, during the spring of 2014. The later died because complications associated with multiorgan failure. Several deaths due to BRBV infection have since been reported United States, and misdiagnosed cases are often undercounted. is member genus Thogotovirus Orthomyxoviridae family, transmitted through Lone Star tick, Amblyomma americanum , North America. Currently, no specific antiviral...
Two goose megriviruses (W18 and HN56) were detected sequenced. Both viruses possessed megrivirus-like genomic features, including unusually long genomes (9840 10 101 nt). W18 HN56 very similar to duck megrivirus (DMV) in the P2 P3 regions, but much less P1 2A1 regions. may be a potential recombinant virus, with distinct region possibly originating from an unknown picornavirus. represent novel type of DMV, showing sequence identity 67 %. Similar levels (64-68 %) also displayed by melegrivirus...
B19V encodes a large nonstructural protein, NS1. Its N-terminal domain (NS1N) consisting of amino acids 1 to 176 binds viral DNA and serves as an endonuclease nick the replication origins, which is pivotal step in rolling-hairpin-dependent replication.
Human bocavirus 1 (HBoV1), an autonomous human parvovirus, causes acute respiratory tract infections in young children. HBoV1 infects well-differentiated (polarized) airway epithelium cultured at air-liquid interface (HAE-ALI). expresses a large nonstructural protein, NS1, that is essential for viral DNA replication. infection of polarized epithelial cells induces damage response (DDR) critical to replication involving repair with error-free Y-family polymerases. NS1 or the isoform NS1-70...
INTRODUCTION Avian influenza has become a serious public health problem. Risk factors for human cases are direct or closed contact with ill, died poultry and live markets (LPMs) exposure. There is high risk of infection frequency visiting LPMs.[1] Robust data animal models have provided support the potential virus transmission by aerosol. The experimental showed that aerosol accounts approximately half A spread events.[2] Thus, identifying circulating viruses in environmental samples,...
Parvoviruses package a linear single-stranded DNA genome with hairpin structures at both ends. It has been thought that terminal sequences are indispensable for viral replication. Here, we provide evidence the hairpin-deleted duplex genomes of human bocavirus 1 (HBoV1) replicate in embryonic kidney 293 (HEK293) cells. We propose an alternative model HBoV1 replication which leading strand can initiate displacement without transfer. The transfection retain minimal origin right end (OriR) but...
ABSTRACT We conducted a genome-wide CRISPR/Cas9 screen in suspension 293 F cells transduced with rAAV5. The highly selected genes revealed after two rounds of screening included the previously reported KIAA0319L , TM9SF2 and RNF121 along cluster involved glycan biogenesis, Golgi apparatus localization, endoplasmic reticulum penetration. In this report, we focused on solute carrier family 35 member A1 ( SLC35A1 ), apparatus-localized cytidine 5’-monophosphate-sialic acid (CMP-SIA)...
A 47-year-old male (Patient A) passenger from Vietnam whose nucleic acid results for coronavirus disease 2019 , also known as severe acute respiratory syndrome 2 (SARS-CoV-2), were positive at Pingxiang Customs on April 29, 2021, and subsequently confirmed by Guangxi CDC laboratories 30.On 30, four accompanying persons B-E) of Patient returned Vietnam.After being tested the laboratory using nasopharyngeal swab tests COVID-19, patients showed COVID-19 positive.All five transported to...
ABSTRACT Bourbon virus (BRBV) was first isolated from a patient hospitalized at the University of Kansas Hospital in 2014. Since then, several deaths have been reported to be caused by BRBV infection Midwest and Southern United States. is tick-borne that widely carried lone star ticks. It belongs genus Thogotovirus Orthomyxoviridae family. Currently, there are no treatments or vaccines available for thogotovirus diseases. In this study, we reconstituted replicon reporter system, composed...