A5101396789- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Sperm and Testicular Function
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Ovarian function and disorders
- CRISPR and Genetic Engineering
- Genomic variations and chromosomal abnormalities
- Sexual Differentiation and Disorders
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Chromosomal and Genetic Variations
- Assisted Reproductive Technology and Twin Pregnancy
- Ectopic Pregnancy Diagnosis and Management
- Cancer-related gene regulation
- Endometriosis Research and Treatment
- Virus-based gene therapy research
- 3D Printing in Biomedical Research
- Ubiquitin and proteasome pathways
- Tissue Engineering and Regenerative Medicine
- Genetic and Kidney Cyst Diseases
- Mesenchymal stem cell research
- Gynecological conditions and treatments
- RNA modifications and cancer
Reproductive & Genetic Hospital CITIC-Xiangya
2016-2025
National Engineering Research Center of Human Stem Cells
2016-2025
Central South University
2016-2025
Hunan Normal University
2019-2025
Xiangya Hospital Central South University
2024
National Health and Family Planning Commission
2018-2024
Hunan Provincial Center for Disease Control and Prevention
2020-2023
BGI Group (China)
2022
CITIC Group (China)
2012-2021
Medical College of Wisconsin
2010
Abstract STUDY QUESTION Are there other pathogenic genes for asthenoteratozoospermia (AT)? SUMMARY ANSWER DNAH3 is a novel candidate gene AT in humans and mice. WHAT IS KNOWN ALREADY major cause of male infertility. Several underlying have been reported; however, the genetic aetiology remains unknown majority affected men. DESIGN, SIZE, DURATION A total 432 patients with were recruited this study. mutations identified by whole-exome sequencing (WES). Dnah3 knockout mice generated using...
Human pre-implantation embryonic development involves extensive changes in chromatin structure and transcriptional activity. Here, we report on LiCAT-seq, a technique that enables simultaneous profiling of accessibility gene expression with ultra-low input cells, map the transcriptome landscapes for human embryos. We observed global difference between sperm all stages embryos, finding accessible regions tend to occur gene-poor genomic regions. Integrative analyses two datasets reveals strong...
Background The genetic causes of the majority male and female infertility caused by human non-obstructive azoospermia (NOA) premature ovarian insufficiency (POI) with meiotic arrest are unknown. Objective To identify cause NOA POI in two affected members from a consanguineous Chinese family. Methods We performed whole-exome sequencing DNA both patients. identified candidate causative gene was further verified Sanger for pedigree analysis this In silico to functionally characterise mutation,...
Background The genetic causes for most male infertility due to severe asthenozoospermia remain unclear. Objective Our objective was identify unknown factors in 47 patients with from 45 unrelated Chinese families. Methods We performed whole exome sequencing of individuals Mutation screening a control cohort 637 individuals, including 219 oligoasthenospermia, 195 non-obstructive azoospermia and 223 fertile controls. Ultrastructural immunostaining analyses patients’ spermatozoa were...
Abstract Psoriasis is a common, chronic immune-mediated systemic disease that had no effective and durable treatment. Mesenchymal stem cells (MSCs) have immunomodulatory properties. Therefore, we performed phase 1/2a, single-arm clinical trial to evaluate the safety efficacy of human umbilical cord-derived MSCs (UMSCs) in treatment psoriasis preliminarily explore possible mechanisms. Seventeen patients with were enrolled received UMSC infusions. Adverse events, laboratory parameters, PASI,...
The cell division cycle associated 8 (CDCA8) gene plays an important role in mitosis. Overexpression of CDCA8 was reported some human cancers and is required for cancer growth progression. We found expression also high ES cells (hESCs) but dropped significantly upon hESC differentiation. However, the regulation has not yet been studied. Here, we characterized promoter identified its cis-elements transcription factors. Three start sites were identified. Reporter assays revealed that activated...
Male infertility due to spermatogenesis defects affects millions of men worldwide. However, the genetic etiology vast majority remains unclear. Here we describe three with primary multiple morphological abnormalities sperm flagella (MMAF) from two unrelated Han Chinese families. We performed whole-exome sequencing (WES) and Sanger on proband family 1, found that he carried novel compound heterozygous missense mutations in dynein axonemal heavy chain 6 (DNAH6) resulted substitution a...
Day 3 cleavage embryo transfer is routine in many assisted reproductive technology centers today. Embryos are usually selected according to cell number, symmetry and fragmentation for transfer. Many studies have showed the relationship between number developmental potential. However, there limited understanding of division behavior their association with A retrospective observational study was conducted investigate how different behaviors affect potential day embryos by time-lapse imaging....
Human embryonic stem cells (hESCs) during long-term culture acquire chromosomal changes similar to those occurring in tumorigenesis. This was raised concerns about the progression from hESCs malignant cells. study aimed investigate chromosomes, cell phenotype, and genes culture-adapted ascertain whether tumorigenic transformation occurred. By cytogenetic analysis we found progressive karyotypic simple complex chHES-3, one of hESC lines established our laboratory, a suboptimal culture. We...
ABSTRACT Objectives To assess the value of transvaginal sonography ( TVS ) in diagnosis heterotopic pregnancy HP first trimester after in‐vitro fertilization with embryo transfer IVF‐ET ). Methods This was a retrospective review women undergoing IVF ‐ ET between January 2005 and December 2011. Women were diagnosed an using if visible intrauterine gestational sac observed any following: (i) inhomogeneous adnexal mass; (ii) empty extrauterine seen as hyperechoic ring; or (iii) yolk and/or...
Primary ovarian insufficiency ( POI ) is the depletion or loss of normal function, which cause infertility in women before age 40 years. Two homozygous germline truncation mutations STAG3 gene had been reported to causes consanguineous families. Here, we aimed identify genetic 2 affected sisters manifested with primary amenorrhea and partial development secondary sexual characters range height a Han Chinese family. Whole‐exome Sanger sequencing identified donor splice‐site mutation...
Background The genetic causes for most male infertility due to severe oligoasthenoteratozoospermia (OAT) remain unclear. Objective To identify the cause of characterised by OAT. Methods Variant screening was performed whole-exome sequencing from 325 infertile patients with OAT and 392 fertile individuals. In silico in vitro analyses were evaluate impacts candidate disease-causing variants. A knockout mouse model generated confirm gene, intracytoplasmic sperm injection (ICSI) used efficiency...
Maternal messenger ribonucleic acids (mRNAs) are driven by a highly orchestrated scheme of recruitment to polysomes and translational activation. However, selecting regulating individual mRNAs for the translation from competitive pool little-known processes. This research shows that maternal eukaryotic initiation factor 4e1b (Eif4e1b) expresses during oocyte-to-embryo transition (OET), deletion Eif4e1b leads multiple defects concerning oogenesis embryonic developmental competence OET. The...