A5037191391- Advanced Electron Microscopy Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- RNA and protein synthesis mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Ion channel regulation and function
- Ion Channels and Receptors
- Epigenetics and DNA Methylation
- Receptor Mechanisms and Signaling
- Connexins and lens biology
- Advanced biosensing and bioanalysis techniques
- Anodic Oxide Films and Nanostructures
- Gynecological conditions and treatments
- Invertebrate Immune Response Mechanisms
- Enzyme Structure and Function
- Energy Harvesting in Wireless Networks
- Molecular Junctions and Nanostructures
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- Genomics and Chromatin Dynamics
- Metamaterials and Metasurfaces Applications
- Renal and related cancers
- Reproductive Biology and Fertility
- Advanced X-ray Imaging Techniques
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
Goethe University Frankfurt
2019-2024
University of Hong Kong
2023-2024
Max Planck Institute of Biophysics
2019-2024
Hong Kong University of Science and Technology
2023-2024
Sir Run Run Shaw Hospital
2021
Zhejiang University
2021
Shanghai Institutes for Biological Sciences
2017
Chinese Academy of Sciences
2017
Center for Excellence in Molecular Cell Science
2017
CRISPR-Cas9 technology has been widely used for genome engineering. Its RNA-guided endonuclease Cas9 binds specifically to target DNA and then cleaves the two strands with HNH RuvC nuclease domains. However, structural information regarding cleavage-activating state of domains remains sparse. Here, we report a 5.2 Å cryo-EM structure in complex sgRNA DNA. This reveals conformational which domain is closest cleavage site. Compared known states, our shows that active site moves toward by about...
Abstract Pentameric ligand-gated ion channels (pLGICs) of the Cys-loop receptor family are key players in fast signal transduction throughout nervous system. They have been shown to be modulated by lipid environment, however underlying mechanism is not well understood. We report three structures 5-HT 3A serotonin (5HT 3 R) reconstituted into saposin-based bilayer discs: a symmetric and an asymmetric apo state, agonist-bound state. In comparison previously published 5HT R conformations...
Cryo-electron tomography combined with subtomogram averaging (StA) has yielded high-resolution structures of macromolecules in their native context. However, StA is not commonplace due to beam-induced sample drift, images poor signal-to-noise ratios (SNR), challenges CTF correction, and limited particle number. Here we address these issues by collecting tilt series a higher electron dose at the zero-degree tilt. Particles interest are then located within reconstructed tomograms, processed...
The TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated various physiological functions. Deficiency of these would cause many diseases including hearing loss. However, their structures roles not yet well understood. In this study, we determine the cryo-electron microscopy (cryo-EM) structure mouse TMEM63C at 3.56 Å, revealed structural differences compared to TMEM63A, TMEM63B, plant orthologues...
Structure of the malaria blood-stage parasite’s main surface antigen reveals putative host cell–binding domains.
Abstract Multimeric membrane proteins are produced in the endoplasmic reticulum and transported to their target membranes which, for ion channels, is typically plasma membrane. Despite availability of many fully assembled channel structures, our understanding assembly intermediates, multimer mechanisms, potential functions non-standard assemblies limited. We demonstrate that pentameric ligand-gated serotonin 5-HT3A receptor (5-HT3AR) can assemble tetrameric forms report structures tetramers...
Abstract The DNA methylation is gradually acquired during oogenesis, a process sustained by successful follicle development. However, the functional roles of methyl-CpG-binding protein 2 (MeCP2), an epigenetic regulator displaying specifical binding with methylated DNA, remains unknown in oogenesis. In this study, we found MeCP2 was highly expressed primordial and primary follicle, but almost undetectable secondary follicles. aged ovary, significantly increased both oocyte granulosa cells....
Recent technological breakthroughs in single-particle cryo-electron microscopy (cryo-EM) enable rapid atomic structure determination of biological macromolecules. A major bottleneck the current single particle cryo-EM pipeline is preparation good quality frozen grids, which mostly a trial-and-error process. Among many issues, preferred orientation and sample damage by air-water interface (AWI) are common practical problems. Here we report method applying metallo-supramolecular branched...
Abstract The TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated various physiological functions. Deficiency of these would cause many diseases including hearing loss. However, their structures roles not yet well understood. In this study, we determined the cryo-EM structure mouse TMEM63C at 3.56 Å, revealed structural differences compared to its plant orthologues OSCAs. Further guided...
ABSTRACT Cryo electron tomography (cryo-ET) combined with subtomogram averaging (StA) enables structural determination of macromolecules in their native context. A few structures were reported by StA at resolution higher than 4.5 Å, however all these are from viral proteins or vesicle coats. Reaching high for a broader range samples is uncommon due to beam-induced sample drift, poor signal-to-noise ratio (SNR) images, challenges CTF correction, limited number particles. Here we propose...