A5078182767- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Genetics and Neurodevelopmental Disorders
- Receptor Mechanisms and Signaling
- Cardiac Ischemia and Reperfusion
- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Pancreatic function and diabetes
- Cellular transport and secretion
- Epilepsy research and treatment
- RNA regulation and disease
- Neuroinflammation and Neurodegeneration Mechanisms
- Retinal Development and Disorders
- Connexins and lens biology
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Lipid Membrane Structure and Behavior
- bioluminescence and chemiluminescence research
- Cannabis and Cannabinoid Research
- Erythrocyte Function and Pathophysiology
- Pharmacological Receptor Mechanisms and Effects
- RNA and protein synthesis mechanisms
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neural Engineering
Model Animal Research Center
2016-2025
Nanjing University
2016-2025
Nanjing Drum Tower Hospital
2019-2025
Guangdong Polytechnic of Science and Technology
2022-2025
Jiangxi Agricultural University
2025
Ningxia Meteorological Bureau
2025
State Key Laboratory of Pharmaceutical Biotechnology
2016-2024
Zhejiang University of Science and Technology
2024
Griffith University
2021-2023
Zhongshan Hospital
2023
Abstract Thirst plays a vital role in the regulation of body fluid homeostasis and if deregulated can be life-threatening. Interoceptive neurons subfornical organ (SFO) are intrinsically osmosensitive their activation by hyperosmolarity is necessary sufficient for generating thirst. However, primary molecules sensing systemic osmolarity these remain elusive. Here we show that mechanosensitive TMEM63B cation channel osmosensor required interoceptive to drive highly expressed excitatory SFO...
Glutamate receptors of the AMPA subtype (AMPARs) mediate fast synaptic transmission in brain. These ionotropic rely on auxiliary subunits known as transmembrane AMPAR regulatory proteins (TARPs) for both trafficking and gating. Recently, a second family binding proteins, referred to cornichons, were identified also proposed function subunits. Cornichons are that modulate expression systems much like TARPs. In present study we compare role cornichons controlling receptor neurons HEK cells...
Long-term potentiation (LTP) is a persistent strengthening of synaptic transmission in the brain and arguably most compelling cellular molecular model for learning memory. Previous work found that both AMPA receptors exogenously expressed kainate are equally capable expressing LTP, despite their limited homology association with distinct auxiliary subunits, indicating LTP far more promiscuous than previously thought. What might these two subtypes glutamate receptor have common? Using...
Hypotonic stress causes the activation of swelling-activated nonselective cation channels (NSCCs), which leads to Ca2+-dependent regulatory volume decrease (RVD) and adaptive maintenance cell volume; however, molecular identities osmosensitive NSCCs remain unclear. Here, we identified TMEM63B as an NSCC activated by hypotonic stress. is enriched in inner ear sensory hair cells. Genetic deletion results necroptosis outer cells (OHCs) progressive hearing loss. Mechanistically, channel mediates...
Significance AMPA receptors (AMPARs) are major postsynaptic that mediate fast excitatory neurotransmission and synaptic plasticity. The proper functioning of AMPARs is essential for brain function; AMPAR dysfunction can cause multiple neurologic disorders, including autism. Native macromolecular complexes associated with a variety auxiliary proteins, α/β-hydrolase domain-containing 6 (ABHD6), which was recently identified. However, the physiological significance ABHD6–AMPAR association has...
Long-term potentiation (LTP) has long been considered as an important cellular mechanism for learning and memory. LTP expression involves NMDA receptor-dependent synaptic insertion of AMPA receptors (AMPARs). However, how AMPARs are recruited anchored at the postsynaptic membrane during remains largely unknown. In this study, using CRISPR/Cas9 to delete endogenous replace them with mutant forms in single neurons, we have found that amino-terminal domain (ATD) GluA1 is required maintenance....
The TMEM63 family proteins (A, B, and C), calcium-permeable channels in animals that are preferentially activated by hypo-osmolality, have been implicated various physiological functions. Deficiency of these would cause many diseases including hearing loss. However, their structures roles not yet well understood. In this study, we determine the cryo-electron microscopy (cryo-EM) structure mouse TMEM63C at 3.56 Å, revealed structural differences compared to TMEM63A, TMEM63B, plant orthologues...
Postoperative cognitive decline (POCD) is a common complication following anesthesia and surgery, especially in elderly patients; however, the precise mechanisms of POCD remain unclear. Here, we investigated whether nicotinamide adenine dinucleotide phosphate (NADPH) oxidase mediated-abnormalities parvalbumin (PV) interneurons play an important role pathophysiology POCD. The animal model was established using isoflurane exploratory laparotomy 16-month-old male C57BL/6 mice. For...
Abstract Minocycline is a broad-spectrum tetracycline antibiotic. A number of preclinical studies have shown that minocycline exhibits neuroprotective effects in various animal models neurological diseases. However, it remained unknown whether effective to prevent neuron loss. To systematically evaluate its effects, was used treat Dicer conditional knockout (cKO) mice which display age-related The drug given mutant prior the occurrence neuroinflammation and neurodegeneration treatment had...
G protein-coupled inward rectifier K(+) (GIRK) channels regulate cellular excitability and neurotransmission. The GIRK are activated by a number of inhibitory neurotransmitters through the protein betagamma subunit (G(betagamma)) after activation receptors inhibited several excitatory phospholipase C. If inhibition is produced PKC, there should be PKC phosphorylation sites in channel proteins. To identify sites, we performed systematic mutagenesis analysis on GIRK4 GIRK1 subunits expressed...
ATP-sensitive K(+) (K(ATP)) channels are activated by several vasodilating hormones and neurotransmitters through the PKA pathway. Here, we show that phosphorylation at Ser1387 of SUR2B subunit is critical for channel activation. Experiments were performed in human embryonic kidney (HEK) 293 cells expressing cloned Kir6.1/SUR2B channel. In whole cell patch, activity was stimulated isoproterenol via activation beta(2) receptors. This effect blocked presence inhibitors adenylyl cyclase or PKA....
The vascular ATP-sensitive K+ (KATP) channel is targeted by a variety of vasoactive substances, playing an important role in tone regulation. Our recent studies indicate that the KATP inhibited oxidative stress via S-glutathionylation. Here we show evidence for molecular basis S-glutathionylation and its structural impact on gating. By comparing oxidant responses Kir6.1/SUR2B with Kir6.2/SUR2B channel, found Kir6.1 subunit was responsible sensitivity. Oxidant screening Kir6.1-Kir6.2 chimeras...
<title>Abstract</title> Despite significant advances in structural and genetic studies, investigations of early embryonic functions, such as brain activity, have long been constrained by technical challenges. Functional ultrasound (fUS) has emerged a breakthrough modality, enabling real-time monitoring activity with exceptional spatial temporal resolution offering unprecedented opportunities for studying functional development. In this study, we used fUS to monitor whole-embryo mice from...
Abstract Accurate timing of myelination is crucial for the proper functioning central nervous system. Here, we identified a de novo heterozygous mutation in TMEM63A (c.1894G>A; p. Ala632Thr) 7-year-old boy exhibiting hypomyelination. A Ca 2+ influx assay suggested that this loss-of-function mutation. To explore how deficiency causes hypomyelination, generated Tmem63a knockout mice. Genetic deletion resulted hypomyelination at postnatal day 14 (P14) arising from impaired differentiation...
The adverse effects of goose astrovirus (GoAstV) on avian growth and health have been widely reported previously, while the stress reactions corresponding mechanism gosling liver responding to GoAstV infection remain not entirely clear. One-day-old goslings inoculated subcutaneously with 2 × 10-6 TCID50 were employed as an experimental model, potential pathways functions investigated by combining morphological, biochemical RNA sequencing (RNA-seq) techniques. Structural functional...