A5041005873- Ion channel regulation and function
- Cardiac electrophysiology and arrhythmias
- Neuroscience and Neuropharmacology Research
- Amino Acid Enzymes and Metabolism
- Nicotinic Acetylcholine Receptors Study
- Metabolism and Genetic Disorders
- Receptor Mechanisms and Signaling
- Heavy metals in environment
- Cancer, Hypoxia, and Metabolism
- Genetic Neurodegenerative Diseases
- Advancements in Battery Materials
- Advanced Battery Materials and Technologies
- Potato Plant Research
- ATP Synthase and ATPases Research
- Plant Stress Responses and Tolerance
- Monoclonal and Polyclonal Antibodies Research
- Advanced Battery Technologies Research
- Ion Transport and Channel Regulation
- Acupuncture Treatment Research Studies
- Environmental Quality and Pollution
- COVID-19 diagnosis using AI
- Exercise and Physiological Responses
- Neuroscience and Neural Engineering
- Neurological diseases and metabolism
- Force Microscopy Techniques and Applications
Chinese Academy of Sciences
2021-2025
Institute of Biophysics
2021-2025
University of Chinese Academy of Sciences
2021-2025
Queen's University Belfast
2024
Ningbo University of Technology
2024
Czech Academy of Sciences, Institute of Biophysics
2021-2024
Ningxia University
2024
China Medical University
2024
Ministry of Education of the People's Republic of China
2022
Northwest University
2022
Abstract Cadmium (Cd) accumulation in rice grain poses a serious threat to human health. While several transport systems have been reported, the complexity of Cd and indicates necessity identifying additional genes, especially those that are responsible for divergence between indica japonica subspecies. Here, we show gene, OsCd1 , belonging major facilitator superfamily is involved root uptake contributes rice. Natural variation with missense mutation Val449Asp . Near-isogenic line tests...
Sodium/proton exchanger 1 (NHE1) is an electroneutral secondary active transporter present on the plasma membrane of most mammalian cells and plays critical roles in regulating intracellular pH volume homeostasis. Calcineurin B-homologous protein (CHP1) obligate binding partner that promotes NHE1 biosynthetic maturation, cell surface expression pH-sensitivity. Dysfunctions either are associated with neurological disorders. Here, we elucidate structures human NHE1-CHP1 complex both inward-...
N-type voltage-gated calcium (CaV) channels mediate Ca2+ influx at presynaptic terminals in response to action potentials and play vital roles synaptogenesis, release of neurotransmitters, nociceptive transmission. Here, we elucidate a cryo-electron microscopy (cryo-EM) structure the human CaV2.2 complex apo, ziconotide-bound, two CaV2.2-specific pore blockers-bound states. The second voltage-sensing domain (VSD) is captured resting-state conformation, trapped by phosphatidylinositol...
Abstract Voltage-gated sodium (Na V ) channels play fundamental roles in initiating and propagating action potentials. Na 1.3 is involved numerous physiological processes including neuronal development, hormone secretion pain perception. Here we report structures of human 1.3/β1/β2 complex with clinically-used drug bulleyaconitine A selective antagonist ICA121431. Bulleyaconitine located around domain I-II fenestration, providing the detailed view site-2 neurotoxin binding site. It partially...
Abstract The low-voltage activated T-type calcium channels regulate cellular excitability and oscillatory behavior of resting membrane potential which trigger many physiological events have been implicated with diseases. Here, we determine structures the human Ca V 3.3 channel, in absence presence antihypertensive drug mibefradil, antispasmodic otilonium bromide antipsychotic pimozide. contains a long bended S6 helix from domain III, positive charged region protruding into cytosol, is...
Abstract Lithium–sulfur (Li–S) batteries are gaining tremendous attention as promising energy storage solutions due to their impressive density and the affordability of sulfur. However, practical use Li–S encounter major obstacles such polysulfide shuttle effect, which leads capacity loss decreased cycling stability. Herein, a polyethylene imidazole/polyacrylonitrile (PVIMPAN) nanofibers‐modified Celgard separator is constructed via facile electrospinning strategy used polysulfides barrier...
Abstract High-voltage-activated R-type Ca V 2.3 channel plays pivotal roles in many physiological activities and is implicated epilepsy, convulsions, other neurodevelopmental impairments. Here, we determine the high-resolution cryo-electron microscopy (cryo-EM) structure of human complex with α2δ1 β1 subunits. The VSD II stabilized resting state. Electrophysiological experiments elucidate that not required for activation, whereas VSDs are essential opening. intracellular gate blocked by...
Abstract Salt-overly-sensitive 1 (SOS1) is a unique electroneutral Na + /H antiporter at the plasma membrane of higher plants and plays central role in resisting salt stress. SOS1 kept resting state with basal activity activated upon phosphorylation. Here, we report structures SOS1. forms homodimer, each monomer composed transmembrane intracellular domains. We find that locked an occluded by shifting lateral-gate TM5b toward dimerization domain, thus shielding binding site. speculate domain...
Urea is a primary nitrogen source used as fertilizer in agricultural plant production and crucial metabolite plants, playing an essential role modern agriculture. In DUR3 proton-driven high-affinity urea transporter located on the plasma membrane. It not only absorbs external low-concentration nutrient but also facilitates transfer by recovering from senescent leaves. Despite its importance, transport mechanism plants remains insufficiently understood. this study, we determine structures of...
Abstract Trigeminal neuropathic pain (TNP) is often resistant to current pharmacotherapy, and there a pressing need develop more efficacious treatments. Capsaicin pungent ingredient of chili peppers specifically activates transient receptor potential vanilloid subtype 1 (TRPV1), Ca 2+ -permeable ion channel. Topical capsaicin invariably induces burning pain. Paradoxically, the followed by prolonged attenuation preexisting pathologic from same region. However, mechanisms underlying...
TMEM120A, a member of the transmembrane protein 120 (TMEM120) family, has pivotal function in adipocyte differentiation and metabolism, may also contribute to sensing mechanical pain by functioning as an ion channel named TACAN. Here we report that expression TMEM120A is not sufficient mediating poking- or stretch-induced currents cells have solved cryo-electron microscopy (cryo-EM) structures human ( Hs TMEM120A) complex with endogenous metabolic cofactor (coenzyme A, CoASH) apo form. forms...
Abstract Sodium-calcium exchanger proteins influence calcium homeostasis in many cell types and participate a wide range of physiological pathological processes. Here, we elucidate the cryo-EM structure human Na + /Ca 2+ NCX1.3 presence specific inhibitor, SEA0400. Conserved ion-coordinating residues are exposed on cytoplasmic face NCX1.3, indicating that observed is stabilized an inward-facing conformation. We show how regulatory calcium-binding domains (CBDs) assemble with...
Sodium-proton exchanger 3 (NHE3/SLC9A3) located in the apical membrane of renal and gastrointestinal epithelia mediates salt fluid absorption regulates pH homeostasis. As an auxiliary regulatory factor NHE proteins, calcineurin B homologous protein 1 (CHP1) facilitates NHE3 maturation, plasmalemmal expression, sensitivity. Dysfunctions are associated with digestive system disorders. Here, we report cryo-electron microscopy structure human NHE3-CHP1 complex its inward-facing conformation. We...
Abstract The fabrication of anion‐coordinated assemblies into functional soft materials remains a major challenge. To this end, four C 2 ‐symmetric anion‐binding ligands equipped with ortho ‐phenylene‐bridged bis(urea) and amine or amide ends were designed, which generated A L 3 triple helical architectures upon self‐assembly phosphate ions. Hierarchical intermolecular hydrogen bonds among the terminal amine/amide groups urea moieties resulted in formation gels. obtained gels further applied...
Consumers increasingly desire healthier low-fat snack options. In this study, potato starch served as the substrate, and varying quantities of Lycium barbarum L. polysaccharide (0, 1, 3, 5 g/100 g) were added to formula chips (FPC). The effect LBPs on sensory qualities, oil content, microstructure, properties FPC-LBPs was examined. results showed that resulted in enhanced acceptability, reduced increased RS lower straight-chain leaching, a gradual increase composite index (CI)...