A5038367614- Cystic Fibrosis Research Advances
- Neonatal Respiratory Health Research
- Inhalation and Respiratory Drug Delivery
- Asthma and respiratory diseases
- Ion Transport and Channel Regulation
- Biochemical and Structural Characterization
- ATP Synthase and ATPases Research
- Ion channel regulation and function
- Engineering Applied Research
- Aquaculture disease management and microbiota
- GNSS positioning and interference
- Antimicrobial Peptides and Activities
- Phosphodiesterase function and regulation
- Innovation in Digital Healthcare Systems
- Signaling Pathways in Disease
- Geophysics and Gravity Measurements
- Marine and Coastal Research
- Legume Nitrogen Fixing Symbiosis
- Inertial Sensor and Navigation
- Air Quality and Health Impacts
McGill University
2012-2021
Sodium/proton exchanger 1 (NHE1) is an electroneutral secondary active transporter present on the plasma membrane of most mammalian cells and plays critical roles in regulating intracellular pH volume homeostasis. Calcineurin B-homologous protein (CHP1) obligate binding partner that promotes NHE1 biosynthetic maturation, cell surface expression pH-sensitivity. Dysfunctions either are associated with neurological disorders. Here, we elucidate structures human NHE1-CHP1 complex both inward-...
Bicarbonate facilitates mucin unpacking and bacterial killing; however, its transport mechanisms in the airways are not well understood. cAMP stimulates anion efflux through cystic fibrosis (CF) transmembrane conductance regulator (CFTR; ABCC7) channel, this is defective CF. The exchanger pendrin (SLC26A4) also mediates HCO3- upregulated by proinflammatory cytokines. Here, we examined CFTR expression their contributions to secretion human nasal bronchial epithelia. In native tissue, both...
CFTR (cystic fibrosis transmembrane conductance regulator) is a tightly regulated anion channel that mediates chloride and bicarbonate in many epithelia other tissues, but whether its regulation varies depending on the cell type has not been investigated. Epithelial expression highest rare cells called ionocytes. We studied control ionocyte-enriched cultures by transducing bronchial basal with adenoviruses encode only eGFP or FOXI1 (forkhead box I1) + as separate polypeptides. dramatically...
The airway mucosal microenvironment is crucial for host defense against inhaled pathogens but remains poorly understood. We report here that the surface normally undergoes surprisingly large excursions in pH during breathing can reach 9.0 inhalation, making it most alkaline fluid body. Transient alkalinization requires luminal bicarbonate and membrane-bound carbonic anhydrase 12 (CA12) antimicrobial. Luminal concentration CA12 expression are both reduced cystic fibrosis (CF), mucus...
The pH of airway epithelial secretions influences bacterial killing and mucus properties is reduced by acidic pollutants, gastric reflux, respiratory diseases such as cystic fibrosis (CF). effect acute acid loads depends on buffer capacity, however the buffering has not been well characterized. In this work we develop a method for titrating micro-scale (30 μl) volumes use it to study fluid secreted human cell line Calu-3, widely used model submucosal gland serous cells. Microtitration curves...
The cystic fibrosis transmembrane conductance regulator (CFTR) is a tightly regulated anion channel that mediates secretion by epithelia and mutated in the disease fibrosis. CFTR forms macromolecular complexes with many proteins; however, little known regarding its associations membrane lipids or regulation of distribution mobility at cell surface. We report here secretagogues (agonists stimulate secretion) such as peptide hormone vasoactive intestinal (VIP) muscarinic agonist carbachol...
Anion exchanger type 2 (AE2 or SLC4A2) is an electroneutral Cl(-)/HCO(3)(-) expressed at the basolateral membrane of many epithelia. It thought to participate in fluid secretion by airway However, role AE2 remains uncertain, due lack specific pharmacological inhibitors, and because it electrically silent therefore does not contribute directly short-circuit current (I(sc)). We have studied Cl(-) epithelial cell line Calu-3. After confirming expression its mRNA protein, a knock-down called...
Bicarbonate plays an important role in airway host defense, however, its transport mechanisms remain uncertain. Here we examined the relative contributions of anion channel CFTR (cystic fibrosis transmembrane conductance regulator, ABCC7) and exchanger pendrin (SLC26A4) to HCO3− secretion by human cell line Calu-3. Pendrin were both detected parental Calu-3 cells, although mRNA protein expression appeared higher for than pendrin. Targeting transcripts with lentiviral shRNA reduced detection...
<p class="Abstract">We have previously identified an antimicrobial peptide called dybowskin2-CDYa (Dy2) in the cutaneous secretion from Chinese frog Rana dybowskii. In this study, we used Dy2 as a template to prepare some novel peptides with improved stability and hydrophobicity. The activities exerted by these against Gram positive negative bacteria were evaluated MIC CFU assays under physiological conditions. One peptide, AWRK6, was 2-4 fold more potent than Dy2, toxic most of...
Bicarbonate facilitates mucin unpacking and bacterial killing however its transport mechanisms in the airways are not well understood. cAMP stimulates anion efflux through CFTR (ABCC7) channel this is defective CF. The exchanger pendrin (SLC26A4) also mediates HCO 3 − upregulated by proinflammatory cytokines. Here we examined expression their contributions to secretion human nasal bronchial epithelia. In native tissue, both proteins were most abundant at apical pole of ciliated surface cells...
GPS 모듈을 탑재한 모바일 단말기는 꾸준히 증가하고 있으나 코드의사거리를 측위에 사용하므로 반송파 위상을 사용하는 수신기에 비해 정확도가 낮다. 특히 다중경로신호는 위상 측위 결과에 더욱 큰 영향을 미치므로 다중경로신호를 검출하여 측위에서 제거하는 방안이 필요하다. 이 연구에서는 다중경로신호의 검출을 위해 위성의 신호세기를 이용하는 방법에 대해 초기 분석을 수행하였다. 개활지환경에서 고도각이 증가함에 따라 신호세기가 증가하는 것이 확인되었고, 주변에 장애물이 있는 지역에서는 시선방향에 차폐요소가 감쇄되어 기록되는 것을 확인하였다. 이러한 현상이 위성이 동일 궤도를 지날 때에도 반복되어 발생하는지 확인하기 재현시간을 적용하여 비교 분석하였다. 그 결과 위성 신호세기 변동 특성은 궤도상의 위치를 거의 완벽하게 재현되었고, 일시적인 아니라 관측 환경에 의해 완벽히 재현되는 결과임을 확인할 수 있었다. 따라서 이용한 검출이 가능할 것으로 판단된다. The number of...