A5045887929- Fungal and yeast genetics research
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- Cystic Fibrosis Research Advances
- American Constitutional Law and Politics
- Antifungal resistance and susceptibility
- Heat shock proteins research
- RNA and protein synthesis mechanisms
- Neonatal Respiratory Health Research
- Fungal Infections and Studies
- Toxin Mechanisms and Immunotoxins
- Bacterial Genetics and Biotechnology
- Plant Reproductive Biology
- Peptidase Inhibition and Analysis
- Protein Kinase Regulation and GTPase Signaling
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- Transgenic Plants and Applications
- Biofuel production and bioconversion
- Signaling Pathways in Disease
- Asthma and respiratory diseases
- Enzyme Production and Characterization
McGill University
2015-2024
Istituto Giannina Gaslini
2020
The Francis Crick Institute
1973-2016
University of British Columbia
2016
GlaxoSmithKline (United Kingdom)
2016
Imperial College London
2016
Concordia University
2011
Inserm
2010
Kyoto University
2008
Japan Science and Technology Agency
2008
GTP phosphorylation of rough microsomes in vitro is limited to four integral membrane proteins. Two these, a phosphoprotein (pp90) and phosphoglycoprotein (pgp35) were purified as complex with two nonphosphorylated glycoproteins, gp25H gp25L. The authenticity this was confirmed using different purification procedures by coimmunoprecipitation. By immunofluorescence reticulated cytoplasmic network revealed for the proteins which similar that Louvard et al. (Louvard, D., Reggio, H., Warren, G....
Membrane and secretory proteins cotranslationally enter are folded in the endoplasmic reticulum (ER). Misfolded or unassembled discarded by a process known as ER-associated degradation (ERAD), which involves their retrotranslocation into cytosol. ERAD substrates frequently contain disulfide bonds that must be cleaved before retrotranslocation. Here, we found an ER-resident protein ERdj5 had reductase activity, of misfolded proteins, accelerated through its physical functional associations...
The ability of the pathogenic fungus Candida albicans to switch from a yeast hyphal morphology in response external signals is implicated its pathogenicity. We used glass DNA microarrays investigate transcription profiles 6333 predicted ORFs cells undergoing this transition and their responses changes temperature culture medium. have identified several genes whose transcriptional are similar those known virulence factors that modulated by growth caused addition serum 37°C temperature. Time...
The human fungal pathogen Candida albicans switches from a budding yeast form to polarized hyphal in response various external signals. This morphogenetic switching has been implicated the development of pathogenicity. We have cloned CaCDC35 gene encoding C. adenylyl cyclase by functional complementation conditional growth defect Saccharomyces cerevisiae cells with mutations Ras1p and Ras2p. It previously shown that these Ras homologues regulate yeast. is highly homologous other cyclases but...
Abstract. Five mammalian members of the gp25L/ emp24/p24 family have been identified as major constituents cis-Golgi network rat liver and HeLa cells. Two these were also found in membranes higher density (corresponding to ER), this correlated with their ability bind COP I vitro. This binding was mediated by a K(X)KXX-like retrieval motif present cytoplasmic domain two members. A second motif, double phenylalanine (FF), all five members, shown participate Sec23 (COP II). is part larger one,...
Candida albicans is a dimorphic human pathogen in which the yeast to hyphal switch may be an important factor virulence mammals. This has recently been shown also kill insects such as Greater Wax Moth Galleria mellonella when injected into haemocoel of insect larvae. We have investigated effect previously characterised C. mutations that influence transition on G. There good correlation between these mutants host and measured through systemic infection mice. Although predominant cellular...
ABSTRACT Extracellular signal-regulated protein kinase (ERK, or mitogen-activated [MAPK]) regulatory cascades in fungi turn on transcription factors that control developmental processes, stress responses, and cell wall integrity. CEK1 encodes a Candida albicans MAPK homolog (Cek1p), isolated by its ability to interfere with the Saccharomyces cerevisiae mating pathway. C. cells deletion of gene are defective shifting from unicellular budding colonial growth mode an agar-invasive hyphal when...
The CST20 gene of Candida albicans was cloned by functional complementation a deletion the STE20 in Saccharomyces cerevisiae . encodes homolog Ste20p/p65 PAK family protein kinases. Colonies C. cells deleted for revealed defects lateral formation mycelia on synthetic solid “Spider” media. However, hyphal development not impaired some other A similar phenotype caused HST7 , encoding S. Ste7p kinase. Overexpression partially complemented Cells were less virulent mouse model systemic...
The pathogenic fungus Candida albicans is capable of responding to a wide variety environmental cues with morphological transition from budding yeast polarized filamentous form. We demonstrate that the Ras homologue C. , CaRas1p, required for this and thereby contributes development pathogenicity. However, CaRas1p not cellular viability. Deletion both alleles CaRAS1 gene caused in vitro defects were reversed by either supplementing growth media cAMP or overexpressing components...
Type I myosins are highly conserved actin-based molecular motors that localize to the actin-rich cortex and participate in motility functions such as endocytosis, polarized morphogenesis, cell migration. The COOH-terminal tail of yeast myosin-I proteins, Myo3p Myo5p, contains an Src homology domain 3 (SH3) followed by acidic domain. SH3 interacted with both Bee1p Vrp1p, homologues human WASP WIP, adapter proteins link actin assembly signaling molecules. Arp2/3 complex subunits, Arc40p...
A mass spectrometric analysis of proteins partitioning into Triton X-114 from purified hepatic Golgi apparatus (84% purity by morphometry, 122-fold enrichment over the homogenate for marker galactosyl transferase) led to unambiguous identification 81 including a novel Golgi-associated protein 34 kDa (GPP34). The membrane complement was resolved SDS-polyacrylamide gel electrophoresis and subjected hierarchical approach using delayed extraction matrix-assisted laser desorption ionization...
Multidrug resistance in mammalian tumor cells is associated with the overexpression of mdr genes encoding P-glycoproteins, which function as drug efflux pumps. A yeast homolog , STE6 mediates export a-factor mating peptide. Yeast MAT a carrying ste6 deletion produce no extracellular and therefore are defective mating. Expression complementary DNA for mouse mdr3 gene strain restored ability to mate. mutation (a serine phenylalanine substitution at amino acid 939) known affect activity product...