A5058413134- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Fungal and yeast genetics research
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Enzyme Production and Characterization
- Monoclonal and Polyclonal Antibodies Research
- Endoplasmic Reticulum Stress and Disease
- Cellular transport and secretion
- RNA Research and Splicing
- Genomics and Phylogenetic Studies
- Microbial Metabolites in Food Biotechnology
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Studies on Chitinases and Chitosanases
- RNA modifications and cancer
- Polysaccharides and Plant Cell Walls
- Entomopathogenic Microorganisms in Pest Control
- Bacterial Genetics and Biotechnology
- Ubiquitin and proteasome pathways
- Trypanosoma species research and implications
- Enzyme Structure and Function
- Escherichia coli research studies
- Microbial Metabolic Engineering and Bioproduction
ETH Zurich
2015-2025
Board of the Swiss Federal Institutes of Technology
2001-2022
École Polytechnique Fédérale de Lausanne
2003-2021
University of California, San Diego
1994-2016
Czech Academy of Sciences, Institute of Microbiology
2004-2016
Hôpital Bretonneau
2016
Centre National de la Recherche Scientifique
2016
Johns Hopkins University
2016
Stony Brook University
2016
The University of Melbourne
2016
Author(s): Varki, Ajit; Cummings, Richard D; Aebi, Markus; Packer, Nicole H; Seeberger, Peter Esko, Jeffrey Stanley, Pamela; Hart, Gerald; Darvill, Alan; Kinoshita, Taroh; Prestegard, James J; Schnaar, Ronald L; Freeze, Hudson Marth, Jamey Bertozzi, Carolyn R; Etzler, Marilynn E; Frank, Martin; Vliegenthart, Johannes Fg; Lutteke, Thomas; Perez, Serge; Bolton, Evan; Rudd, Pauline; Paulson, James; Kanehisa, Minoru; Toukach, Philip; Aoki-Kinoshita, Kiyoko F; Dell, Anne; Narimatsu, Hisashi;...
N-linked protein glycosylation is the most abundant posttranslation modification of secretory proteins in eukaryotes. A wide range functions are attributed to glycan structures covalently linked asparagine residues within asparagine-X-serine/threonine consensus sequence (Asn-Xaa-Ser/Thr). We found an system bacterium Campylobacter jejuni and demonstrate that a functional pathway could be transferred into Escherichia coli . Although bacterial N-glycan differs structurally from its eukaryotic...
Campylobacter jejuni has a general N-linked protein glycosylation system that can be functionally transferred to Escherichia coli. In this study, we engineered E. coli cells in way two different pathways, N-glycosylation and lipopolysaccharide (LPS) biosynthesis, converge at the step which PglB, key enzyme of C. system, transfers O polysaccharide from lipid carrier (undecaprenyl pyrophosphate) an acceptor protein. PglB was only bacterial machinery both necessary sufficient for transfer. The...
Abstract The Symbol Nomenclature for Glycans (SNFG) is a community-curated standard the depiction of monosaccharides and complex glycans using various colored-coded, geometric shapes, along with defined text additions. It hosted by National Center Biotechnology Information (NCBI) at NCBI-Glycans Page (www.ncbi.nlm.nih.gov/glycans/snfg.html). Several changes have been made to SNFG page in past year update rules depicting SNFG, include more examples use, particularly non-mammalian organisms,...
Remember the sugar when making proteins Eukaryotes have an elaborate trafficking and quality-control system for secreted glycoproteins. The glycosylation pathway begins in endoplasmic reticulum with enzyme oligosaccharyltransferase (OST), which attaches a long chain of sugars to asparagine residues target proteins. Wild et al. report cryo-electron microscopy structure yeast OST, includes eight separate membrane central catalytic subunit contains binding sites substrates is flanked by...
In Saccharomyces cerevisiae, transfer of N-linked oligosaccharides is immediately followed by trimming ER-localized glycosidases. We analyzed the influence specific oligosaccharide structures for degradation misfolded carboxypeptidase Y (CPY). By studying reactions in vivo, we found that removal terminal α1,2 glucose and first α1,3 glucosidase I II respectively, occurred rapidly, whereas mannose cleavage mannosidase was slow. Transport maturation correctly folded CPY not dependent on...
The yeast non-Mendelian genetic factor [PSI], which enhances the efficiency of tRNA-mediated nonsense suppression in Saccharomyces cerevisiae , is thought to be an abnormal cellular isoform Sup35 protein. Genetic studies have established that N-terminal part protein sufficient for genesis as well maintenance [PSI]. Here we demonstrate polypeptide fragment consisting residues 2–114 Sup35p, Sup35pN, spontaneously aggregates form thin filaments vitro . show a β-sheet-type circular dichroism...
One of the most powerful techniques for attributing functions to genes in uni- and multicellular organisms is comprehensive analysis mutant traits. In this study, systematic quantitative analyses traits are achieved budding yeast Saccharomyces cerevisiae by investigating morphological phenotypes. Analysis fluorescent microscopic images triple-stained cells makes it possible treat variations as Deletion nearly half not essential growth affects these Similar phenotypes caused deletions...
Human cells treated with interferon synthesize two proteins that exhibit high homology to murine Mx1 protein, which has previously been identified as the mediator of interferon-induced cellular resistance mouse against influenza viruses. Using cDNA a hybridization probe, we have isolated clones originating from distinct human Mx genes, designated MxA and MxB. In fibroblasts, expression MxB is strongly induced by alpha (IFN-alpha), IFN-beta, Newcastle disease virus, and, much lesser extent,...
ABSTRACT The entomopathogenic fungus Neozygites parvispora (Entomophthorales: Zygomycetes) grows in vitro as irregularly rod-shaped hyphal bodies a complex medium. In order to simplify the medium composition and determine growth-promoting compounds for cultivation of this fungus, we were looking rapid quantitative method estimate number living cells small volumes liquid culture. A colorimetric determination cell densities using MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium...
The Saccharomyces cerevisiae Wbp1 protein is an endoplasmic reticulum (ER), type I transmembrane which contains a cytoplasmic dilysine (KKXX) motif. This motif has previously been shown to direct Golgi-to-ER retrieval of membrane proteins in mammalian cells (Jackson, M. R., T. Nilsson, and P. A. Peterson. 1993. J. Cell Biol. 121: 317-333). To analyze the role this yeast, we constructed SUC2-WBP1 chimera consisting coding sequence for normally secreted glycoprotein invertase fused COOH...