A5069319935- Polysaccharides and Plant Cell Walls
- Glycosylation and Glycoproteins Research
- Microbial Metabolites in Food Biotechnology
- Biofuel production and bioconversion
- Genomics and Phylogenetic Studies
- Carbohydrate Chemistry and Synthesis
- Enzyme Production and Characterization
- Polysaccharides Composition and Applications
- Plant nutrient uptake and metabolism
- Biomedical Text Mining and Ontologies
- Food composition and properties
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Plant Gene Expression Analysis
- Semantic Web and Ontologies
- Plant Molecular Biology Research
- Bayesian Modeling and Causal Inference
- Proteoglycans and glycosaminoglycans research
- Data Management and Algorithms
- Advanced Proteomics Techniques and Applications
- Proteins in Food Systems
- Scientific Computing and Data Management
- Spectroscopy and Chemometric Analyses
- Microbial Metabolic Engineering and Bioproduction
- Simulation Techniques and Applications
National Institutes of Health
2023-2024
National Institute on Aging
2023-2024
Institute on Aging
2024
University of Georgia
2012-2022
John Wiley & Sons (United States)
2022
Oak Ridge National Laboratory
2012-2020
Sandia National Laboratories California
2020
Carbohydrate Competence Center
2010-2017
United States Department of Energy
2008-2015
Pasadena City College
2014
Author(s): Varki, Ajit; Cummings, Richard D; Aebi, Markus; Packer, Nicole H; Seeberger, Peter Esko, Jeffrey Stanley, Pamela; Hart, Gerald; Darvill, Alan; Kinoshita, Taroh; Prestegard, James J; Schnaar, Ronald L; Freeze, Hudson Marth, Jamey Bertozzi, Carolyn R; Etzler, Marilynn E; Frank, Martin; Vliegenthart, Johannes Fg; Lutteke, Thomas; Perez, Serge; Bolton, Evan; Rudd, Pauline; Paulson, James; Kanehisa, Minoru; Toukach, Philip; Aoki-Kinoshita, Kiyoko F; Dell, Anne; Narimatsu, Hisashi;...
A revised system of abbreviated names is proposed for xyloglucan‐derived oligosaccharides. Each (1→4)‐linked β‐ d ‐glucosyl residue (and the reducing terminal ‐glucose moiety) backbone given a one‐letter code according to its substituents. The name oligosaccharide consists these letters listed in sequence from non‐reducing terminus backbone.
Mutations of Arabidopsis thaliana IRREGULAR XYLEM8 (IRX8) and IRX9 were previously shown to cause a collapsed xylem phenotype decreases in xylose cellulose cell walls. In this study, we characterized IRX8 performed chemical structural analyses glucuronoxylan (GX) from irx8 irx9 plants. are expressed specifically cells undergoing secondary wall thickening, their encoded proteins targeted the Golgi, where GX is synthesized. 1H-NMR spectroscopy showed that reducing end contains glycosyl...
Abstract A collection of 130 new plant cell wall glycan-directed monoclonal antibodies (mAbs) was generated with the aim facilitating in-depth analysis glycans. An enzyme-linked immunosorbent assay-based screen against a diverse panel 54 polysaccharides used to characterize binding patterns these mAbs, together 50 other previously Hierarchical clustering group mAbs based on polysaccharide recognition observed. The mAb groupings in resulting cladogram were further verified by...
Abstract The Symbol Nomenclature for Glycans (SNFG) is a community-curated standard the depiction of monosaccharides and complex glycans using various colored-coded, geometric shapes, along with defined text additions. It hosted by National Center Biotechnology Information (NCBI) at NCBI-Glycans Page (www.ncbi.nlm.nih.gov/glycans/snfg.html). Several changes have been made to SNFG page in past year update rules depicting SNFG, include more examples use, particularly non-mammalian organisms,...
Summary Xylan is the third most abundant glycopolymer on earth after cellulose and chitin. As a major component of wood, grain forage, this natural biopolymer has far‐reaching impacts human life. This highly acetylated cell wall polysaccharide vital plant wall, which functions as molecular scaffold, providing plants with mechanical strength flexibility. Mutations that impair synthesis xylan backbone give rise to fail grow normally because collapsed xylem cells in vascular system. Phenotypic...
Article Free Access Share on The WebBook and the Web Forager: an information workspace for World-Wide Authors: Stuart K. Card Xerox Palo Alto Research Center, 3333 Coyote Hill Road Alto, California CaliforniaView Profile , George G. Robertson William York Authors Info & Claims CHI '96: Proceedings of SIGCHI Conference Human Factors in Computing SystemsApril 1996 Pages 111–ff.https://doi.org/10.1145/238386.238446Online:13 April 1996Publication History 178citation2,410DownloadsMetricsTotal...
Summary Cellulose and xyloglucan (XG) assemble to form the cellulose/XG network, which is considered be dominant load‐bearing structure in growing cell walls of non‐graminaceous land plants. We have extended most commonly accepted model for macromolecular organization XG this based on structural quantitative analysis three distinct fractions that can differentially extracted from isolated etiolated pea stems. Approximately 8% dry weight these consists solubilized by treatment with a...
Secondary walls in vessels and fibers of dicotyledonous plants are mainly composed cellulose, xylan, lignin. Although genes involved biosynthesis cellulose lignin have been intensively studied, little is known about participating xylan synthesis. We found that Arabidopsis thalianafragile fiber8 (fra8) defective The fra8 mutation caused a dramatic reduction fiber wall thickness decrease stem strength. FRA8 was to encode member glycosyltransferase family 47 exhibits high sequence similarity...
Xyloglucan, isolated from the soluble extracellular polysaccharides of suspension-cultured sycamore (Acer pseudoplatanus) cells, was digested with an endo-beta-1,4-glucanase purified culture fluid Trichoderma viride. A nonasaccharide-rich Bio-Gel P-2 fraction this digest inhibited 2,4-dichlorophenoxyacetic-acid-stimulated elongation etiolated pea stem segments. The inhibitory activity oligosaccharide exhibited a well-defined concentration optimum between 10(-2) and 10(-1) micrograms per...
A full-length c-DNA encoding a xyloglucan-specific endo -β-1,4-glucanase (XEG) has been isolated from the filamentous fungus Aspergillus aculeatus by expression cloning in yeast. The colonies expressing functional XEG were identified on agar plates containing azurine-dyed crosslinked xyloglucan. cDNA was isolated, sequenced, cloned into an vector, and transformed oryzae for heterologous expression. recombinant enzyme purified to apparent homogeneity anion-exchange gel permeation...
Recent advances in chemical and physical techniques have shed considerable light on the molecular architecture of primary cell walls higher plants. According to current models, wall is composed at least two independent polysaccharide networks. The cellulose-xyloglucan network considered be an important load-bearing structure wall. Gaps are filled by a pectic polysaccharides that may control porosity
Glycan structures covalently attached to proteins and lipids play numerous roles in mammalian cells, including protein folding, targeting, recognition, adhesion at the molecular or cellular level. Regulating abundance of glycan on glycoproteins glycolipids is a complex process that depends factors. Most models for regulation hypothesize transcriptional control enzymes involved synthesis, modification, catabolism determines diversity. However, few broad-based studies have examined...
The hemicellulose 4- O -methyl glucuronoxylan is one of the principle components present in secondary cell walls eudicotyledonous plants. However, biochemical mechanisms leading to formation this polysaccharide and effects modulating its structure on physical properties wall are poorly understood. We have identified functionally characterized an Arabidopsis methyltransferase (GXMT) that catalyzes -methylation glucuronic acid substituents polysaccharide. AtGXMT1, which was previously...
Xyloglucan is a well-characterized hemicellulosic polysaccharide that present in the cell walls of all seed-bearing plants. The avascular and seedless vascular plants are also believed to contain xyloglucan. However, these xyloglucans have not been structurally characterized. This lack information an impediment understanding changes xyloglucan structure occurred during land plant evolution. In this study, were isolated from (liverworts, mosses, hornworts) (club spike mosses ferns fern...