A5086878581- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Galectins and Cancer Biology
- Proteoglycans and glycosaminoglycans research
- Genomics and Phylogenetic Studies
- RNA modifications and cancer
- Biochemical and Molecular Research
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Molecular Biology Techniques and Applications
- Calpain Protease Function and Regulation
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- RNA Research and Splicing
- Muscle Physiology and Disorders
- Liver physiology and pathology
- Polysaccharides and Plant Cell Walls
- Helicobacter pylori-related gastroenterology studies
- Plant Gene Expression Analysis
- Pregnancy and Medication Impact
- Genomics and Rare Diseases
- Invertebrate Immune Response Mechanisms
- Sphingolipid Metabolism and Signaling
- Congenital heart defects research
- Silk-based biomaterials and applications
University of Georgia
2012-2022
Center for Drug Evaluation and Research
2018
United States Food and Drug Administration
2018
Japan Science and Technology Agency
2007
Chiba University
2007
U.S. Horticultural Research Laboratory
1995
Glycan structures covalently attached to proteins and lipids play numerous roles in mammalian cells, including protein folding, targeting, recognition, adhesion at the molecular or cellular level. Regulating abundance of glycan on glycoproteins glycolipids is a complex process that depends factors. Most models for regulation hypothesize transcriptional control enzymes involved synthesis, modification, catabolism determines diversity. However, few broad-based studies have examined...
We have isolated a full-length cDNA clone encoding human α1,2-mannosidase that catalyzes the first mannose trimming step in processing of mammalian Asn-linked oligosaccharides. This enzyme has been proposed to regulate timing quality control glycoprotein degradation endoplasmic reticulum (ER) eukaryotic cells. Human expressed sequence tag clones were identified by similarity and yeast oligosaccharide-processing mannosidases, coding region putative mannosidase homolog was combination 5′-rapid...
Glycosaminoglycans (GAGs) play a critical role in binding and activation of growth factors involved cell signaling for developmental biology. The biosynthetic pathways GAGs have been elucidated over the past decade now analytical methodology makes it possible to determine GAG composition as few 10 million cells. A glycomics approach was used examine content, composition, level transcripts encoding enzymes murine embryonic stem cells (mESCs) differentiate embryoid bodies (EBs) extraembryonic...
Abstract Epithelial ovarian cancer is the deadliest female reproductive tract malignancy in Western countries. Less than 25% of cases are diagnosed when confined, however, pointing to critical need for early diagnostics cancer. Identifying changes that occur glycome cells may provide an avenue develop a new generation potential biomarkers detection this disease. We performed glycotranscriptomic analysis endometrioid carcinoma using human tissue, as well newly developed mouse model mimics Our...
Heparan sulfate (HS) has been implicated in regulating cell fate decisions during differentiation of embryonic stem cells (ESCs) into advanced types. However, the necessity and underlying molecular mechanisms HS early lineage are still largely unknown. In this study, we examined potential EXT1−/− mouse ESCs (mESCs), that deficient HS, to differentiate primary germ layer cells. We observed mESCs lost their competence failed Pax6+-neural precursor mesodermal More detailed analyses highlighted...
NOTCH1 is a transmembrane receptor that initiates cell-cell signaling pathway controlling various cell fate specifications in metazoans. The addition of O-fucose by protein O-fucosyltransferase 1 (POFUT1) to epidermal growth factor-like (EGF) repeats the extracellular domain essential for function, and modification with GlcNAc Fringe family glycosyltransferases modulates Notch activity. Prior cell-based studies showed POFUT1 modifies EGF containing appropriate consensus sequence at high...
The severe pediatric disorder mucolipidosis II (ML-II; also known as I-cell disease) is caused by defects in mannose 6-phosphate (Man-6-P) biosynthesis. Patients with ML-II exhibit multiple developmental defects, including skeletal, craniofacial and joint abnormalities. To date, the molecular mechanisms that underlie these clinical manifestations are poorly understood. Taking advantage of a zebrafish model ML-II, we previously showed cartilage morphogenesis this associated altered...
The glycosylphosphatidylinositol (GPI) anchor is a glycan and lipid posttranslational modification added to proteins in the endoplasmic reticulum. Certain enzymes within GPI biosynthetic pathway, particularly subunits of transamidase,
Fringes are glycosyltransferases that transfer a GlcNAc to O-fucose residues on Epidermal Growth Factor-like (EGF) repeats. Three exist in mammals: LUNATIC FRINGE (LFNG), MANIC (MFNG), and RADICAL (RFNG). Fringe modification of EGF repeats the NOTCH1 (N1) extracellular domain modulates activation N1 signaling. Not all modified by Fringes; some one or two others not at all. The distinct effects activity depend which is expressed cell. However, little data available effect more than has...
Ceramides (Cers) are important in embryogenesis, but no comprehensive analysis of gene expression for Cer metabolism nor the amounts and subspecies has been conducted with an often used model: mouse embryonic stem cells (mESCs) versus embroid bodies (EBs). Measuring mRNA levels by quantitative RT-PCR respective metabolites LC-ESI/MS/MS, notable differences between R1 mESCs EBs were: have higher mRNAs CerS1 CerS3, which synthesize C18- C>or=24-carbons dihydroceramides (DH)Cer, respectively;...
Polysialic acid (PSA) is a carbohydrate polymer of repeating α-2,8 sialic residues that decorates multiple targets, including neural cell adhesion molecule (NCAM). PST and STX encode the two enzymes responsible for PSA modification target proteins in mammalian cells, but despite widespread polysialylation embryonic development, majority studies have focused strictly on role neurogenesis. Using human pluripotent stem cells (hPSCs), we revisited developmental show early progenitors three germ...
Mucin-type O-glycans decorate >80% of secretory and cell surface proteins contribute to health disease.However, dynamic alterations in the O-glycome are poorly understood because current O-glycomic methodologies not sufficiently sensitive nor quantitative.Here we describe a novel isotope labeling approach termed Isotope-Cellular Reporter Amplification (ICORA) amplify analyze from cells.In this approach, cells incubated with Ac 3 GalNAc-Bn (Ac GalNAc-[ 1 H 7 ]Bn) or heavy labeled D7 2 D...
Loblolly pine (Pinus taeda L.), the most widely planted tree species in United States, is an important source of wood and fibers for a multitude consumer products. Wood are primarily composed secondary cell walls, cellulose, hemicelluloses lignin major components wood. Fiber morphology wall composition determinants properties. We used comparative genomics to identify putative genes cellulose hemicellulose synthesis loblolly that homologous implicated angiosperms. Sequences encoding synthase...
Background: Her-2-induced mammary tumor onset is significantly delayed in GnT-V knock-out mice.Results: The gene expression of the Pcdhβ cluster up-regulated her-2-induced tumors with deletion.Conclusion: Up-regulation one mechanisms for reduced her-2-mediated tumorigenesis resulting from deletion.Significance: Our findings shed new light on molecular effects tumorigenesis. mice. Results: deletion. Conclusion: Significance: