A5010979187- Growth Hormone and Insulin-like Growth Factors
- Biosensors and Analytical Detection
- Glycosylation and Glycoproteins Research
- Diabetes Treatment and Management
- Metabolism, Diabetes, and Cancer
- Influenza Virus Research Studies
- Chronic Kidney Disease and Diabetes
- Pituitary Gland Disorders and Treatments
- Renal Diseases and Glomerulopathies
- Respiratory viral infections research
- Advanced biosensing and bioanalysis techniques
- Monoclonal and Polyclonal Antibodies Research
- Diabetes Management and Research
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- Multiple Myeloma Research and Treatments
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Bacterial Infections and Vaccines
- Pancreatic function and diabetes
- Antiplatelet Therapy and Cardiovascular Diseases
- Extracellular vesicles in disease
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Childhood Cancer Survivors' Quality of Life
Fujian Agriculture and Forestry University
2025
WuXi AppTec (China)
2025
Affiliated Hospital of Hebei University
2016-2025
Weifang Medical University
2025
Capital Institute of Pediatrics
2020-2024
Central South University
2020-2023
Southern Medical University
2011-2023
Nanfang Hospital
2011-2023
University of Pittsburgh
2023
National Clinical Research Center for Digestive Diseases
2023
Crucial roles of hematologic and immunologic responses in progression coronavirus disease 2019 (COVID-19) remain largely unclear.We sought to address the dynamic changes biomarkers their associations with severity outcomes COVID-19.A retrospective study including 548 patients COVID-19 clarified outcome (discharged or deceased) from a national cohort China was performed. Cross-sectional longitudinal variations were compared different analyzed.On admission, counts lymphocytes, T-cell subsets,...
The first step in influenza infection of the human respiratory tract is binding virus to sialic (Sia) acid terminated receptors. different strains for receptor determined by α linkage galactose and adjacent glycan structure. In this study N- O-glycan composition lung, bronchus nasopharynx was characterized mass spectrometry. Analysis showed that there a wide spectrum both Sia α2-3 α2-6 glycans lung bronchus. This structural data then utilized combination with from 4 published arrays assess...
Importance The role of mycophenolate mofetil (MMF) in management immunoglobulin A nephropathy (IgAN) remains highly controversial. Objective To evaluate the efficacy and safety MMF patients with IgAN at high risk kidney function loss. Design, Setting, Participants This randomized clinical trial open-label, blinded end-point design was conducted among adults IgAN, proteinuria greater than 1.0 g/d, estimated glomerular filtration rate (eGFR) 30 less 60 mL/min/1.73m 2 or persistent hypertension...
Transforming growth factor-β (TGF-β) is a well-established central mediator of renal fibrosis, common outcome almost all progressive chronic kidney diseases. Here, we identified poorly conserved and kidney-enriched long noncoding RNA in TGF-β1-stimulated human tubular epithelial cells fibrotic kidneys, which termed TGF-β/Smad3-interacting (lnc-TSI). Lnc-TSI was transcriptionally regulated by Smad3 specifically inhibited TGF-β-induced phosphorylation downstream profibrotic gene expression....
Abstract The spike (S) glycoprotein in the envelope of SARS-CoV-2 is densely glycosylated but functions its glycosylation are unknown. Here we demonstrate that S recognized a glycan-dependent manner by multiple innate immune receptors including mannose receptor MR/CD206, DC-SIGN/CD209, L-SIGN/CD209L, and MGL/CLEC10A/CD301. Single-cell RNA sequencing analyses indicate such highly expressed cells tissues susceptible to infection. Binding above characterized affinities picomolar range...
Purpose Recurrent renal cell carcinoma(reRCC) is associated with poor prognosis and the underlying mechanism not yet clear. A comprehensive understanding of tumor microenvironment (TME) reRCC may aid in designing effective anticancer therapies, including immunotherapies. Single-cell transcriptomics holds great promise for investigating TME, however, this technique has been used reRCC. Here, we aimed to explore difference TME gene expression pattern between primary RCC (pRCC) at single-cell...
Abstract The current outbreak of monkeypox virus (MPXV) has become a public health emergency international concern that highlights the need for rapid, sensitive MPXV diagnostic assays. Here, we combined isothermal multiple cross displacement amplification (MCDA) with nanoparticle‐based lateral flow biosensor (LFB) to devise test diagnosis infection (called MPXV‐MCDA‐LFB) and differentiation West Central African isolates. MPXV‐MCDA‐LFB protocol conducts MCDA reaction DNA templates followed by...
Influenza A viruses can bind sialic acid-terminating glycan receptors, and species specificity is often correlated with acid linkage avian strains recognizing α2,3-linked sialylated glycans mammalian preferring α2,6-linked glycans. These paradigms derive primarily from studies involving erythrocyte agglutination, binding to synthetic receptor analogs or undefined surface markers on cells tissues. Here, we present the first examination of N-glycome human lung for identifying natural receptors...
Abstract Context Safety concerns have been raised regarding premature mortality, diabetes, neoplasia, and cerebrovascular disease in association with GH therapy. Objective To assess incidence of key safety outcomes. Design Prospective, multinational, observational study (1999 to 2015). Setting A total 22,311 GH-treated children from 827 investigative sites 30 countries. Patients Children growth disorders. Interventions treatment. Main outcome measures Standardized mortality ratio (SMR)...
Abstract Glycans within human lungs are recognized by many pathogens such as influenza A virus (IAV), yet little is known about their structures. Here we present the first analysis of N- and O- glycosphingolipid-glycans from total lungs, along with histological analyses IAV binding. The N-glycome lung contains extremely large complex-type N-glycans linear poly-N-acetyllactosamine (PL) [-3Galβ1–4GlcNAcβ1-] n extensions, which predominantly terminated in α2,3-linked sialic acid. By contrast,...
Mycoplasma pneumoniae (MP) is an important causative agent of morbidity and mortality among all age groups, especially patients extreme ages. Improved readily available tests for accurate, sensitive rapid diagnosis MP infection sorely needed. Here, we developed a CRISPR-Cas12b-based detection platform on the basis recombinase polymerase amplification (RPA) rapid, simple, accurate infection, named MP-RPA-CRISPR. The RPA was employed amplifying community-acquired respiratory distress syndrome...
The ongoing outbreak of the monkeypox, caused by monkeypox virus (MPXV), has been a public health emergency international concern, indicating an urgent need for rapid and sensitive MPXV detection. Here, we designed diagnostic test based on loop-mediated isothermal amplification (LAMP) nanoparticle-based lateral flow biosensor(LFB)for diagnosis infection, termed MPX-LAMP-LFB. A set six LAMP primers was ATI gene MPXV, templates performed at 63°C only 40 min. results were rapidly visually...
The endocannabinoid system has multiple effects. Through interacting with cannabinoid receptor type 1 and 2, this can greatly affect disease progression. Previously, we showed that activated 2 (CB2) mediated kidney fibrosis. However, the underlying mechanisms remain underdetermined. Here, report CB2 was upregulated predominantly in tubular epithelial cells unilateral urinary obstruction ischemia-reperfusion injury models mice, patients a variety of diseases. expression closely correlated...
The lack of a tool for predicting the response to immunosuppressive therapy in IgA nephropathy (IgAN) limits patient-specific risk stratification and early treatment decision making. Models immunosuppression IgAN that can be applied at time kidney biopsy are needed.This prospective cohort study involved 621 Chinese patients with who were high disease progression had persistent proteinuria ≥1 g/d, despite 3 months optimized supportive care renin-angiotensin system inhibitors. Participants...
Acute kidney injury (AKI) is increasingly identified as a crucial risk factor for progression to CKD. However, the factors governing AKI CKD remain largely unknown. By high-throughput RNA sequencing, we found that Neat1_2, transcript variant of Neat1, was upregulated in 40-min ischemia/reperfusion (IRI), which resulted development renal fibrotic lesions. The upregulation Neat1_2 hypoxia-treated TECs attributed p53 transcriptional regulation. Gain- and loss-of-function studies, both vitro...
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a novel detection platform for diagnosis, termed MTB-MCDA-CRISPR. MTB-MCDA-CRISPR pre-amplified specific sdaA gene MTB MCDA, and MCDA results were then decoded detection, resulting in simple visual fluorescent signal readouts. A set standard...
Abstract Background It has been documented that aerobic exercise (AE) a positive effect on improving cognitive function in type 2 diabetes (T2DM) patients. Here, we tried to explore how AE regulates the expression of long non-coding RNA serum-exosomes (Exos), thereby affecting impairment T2DM mice as well its potential molecular mechanism. Methods mouse models were constructed, and serum-Exos isolated for whole transcriptome sequencing screen differentially expressed lncRNA mRNA, followed by...
Pseudomonas aeruginosa (P. aeruginosa) is an important bacterial pathogen involved in a wide range of infections and antimicrobial resistance. Rapid reliable diagnostic methods are vital for early identification, treatment, stop P. infections. In this study, we developed simple, rapid, sensitive, specific detection platform infection diagnosis. The method integrated recombinase polymerase amplification (RPA) technique with clustered regularly interspaced short palindromic repeat...