A5044257559- Biosensors and Analytical Detection
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Bacterial Infections and Vaccines
- Respiratory viral infections research
- Virus-based gene therapy research
- Cholesterol and Lipid Metabolism
- Mosquito-borne diseases and control
- Viral Infections and Immunology Research
- Infective Endocarditis Diagnosis and Management
- Pneumonia and Respiratory Infections
- Fatty Acid Research and Health
- Biochemical effects in animals
- Bacterial biofilms and quorum sensing
- Cytomegalovirus and herpesvirus research
Capital Institute of Pediatrics
2022-2024
Southwest University
2024
Mycoplasma pneumoniae (MP) is an important causative agent of morbidity and mortality among all age groups, especially patients extreme ages. Improved readily available tests for accurate, sensitive rapid diagnosis MP infection sorely needed. Here, we developed a CRISPR-Cas12b-based detection platform on the basis recombinase polymerase amplification (RPA) rapid, simple, accurate infection, named MP-RPA-CRISPR. The RPA was employed amplifying community-acquired respiratory distress syndrome...
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a novel detection platform for diagnosis, termed MTB-MCDA-CRISPR. MTB-MCDA-CRISPR pre-amplified specific sdaA gene MTB MCDA, and MCDA results were then decoded detection, resulting in simple visual fluorescent signal readouts. A set standard...
Pseudomonas aeruginosa (P. aeruginosa) is an important bacterial pathogen involved in a wide range of infections and antimicrobial resistance. Rapid reliable diagnostic methods are vital for early identification, treatment, stop P. infections. In this study, we developed simple, rapid, sensitive, specific detection platform infection diagnosis. The method integrated recombinase polymerase amplification (RPA) technique with clustered regularly interspaced short palindromic repeat...
Mycoplasma pneumoniae (MP), the causative agent of MP pneumonia (MPP), has posed a substantial burden to public health owing lack rapid and effective diagnostic methods. Here, we designed loop-mediated isothermal amplification (LAMP)-based assay, termed LAMP, combined with nanoparticle-based lateral flow biosensor (LAMP-LFB) for sensitive diagnosis MP.-LAMP-LFB included set six primers targeting community-acquired respiratory distress syndrome (CARDS) toxin gene was performed optimally at...
Mycoplasma pneumoniae (MP), which is responsible for a majority of community-acquired pneumonia (CAP) in children, has been largely underestimated. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design novel detection platform termed MP-MCDA-CRISPR assay MP infection diagnosis and clinical application. The amplified the CARDS gene by MCDA method, followed trans-cleavage reporter molecular upon formation...
Moraxella catarrhalis (M. catarrhalis) was an underestimated respiratory infection pathogen that has been largely overlooked. The limited availability of rapid and sensitive detection methodologies hindered M. diagnostic in clinical settings contributed to its underestimation. To address this issue, we devised two recombinase polymerase amplification (RPA)-based assays for rapid, reliable catarrhalis, termed catarrhalis-RPA-Flu catarrhalis-RPA-LFB, which utilized fluorescence...
Haemophilus influenzae is an opportunistic pathogen usually causing bacteremia, meningitis, and pneumonia in children. Here, we developed a method based on multiple cross displacement amplification (MCDA) real-tme fluorescence technique for rapid detection of H. influenzae. A set 10 primers was designed the real-time MCDA reaction, core primer modified with restriction endonuclease recognition sequence, fluorescent, quencher according to principle assay. The reactions were performed using...
Mycoplasma pneumoniae is a significant pathogen responsible for community-acquired pneumonia, predominantly affecting children and adolescents. Here, we devised rapid method M. that combined multiple cross displacement amplification (MCDA) with real-time fluorescence technology. A set of ten primers, which were specifically designed detection, employed in MCDA reaction. Of these, one primer incorporated restriction endonuclease recognition sequence, fluorophore, quencher, facilitating...
Abstract Investigating the anti‐inflammatory effects of bioactive components present in cold‐pressed rapeseed oil through use network pharmacology and molecular docking methods. The were identified by liquid chromatography‐mass spectrometry. We then conducted Gene Ontology Kyoto Encyclopedia Genes Genomes pathway enrichment analysis using bioinformatics databases on overlapping targets affected active inflammation. Finally, was used to predict interactions between core key targets. Analysis...