A5050393004- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Antibiotic Resistance in Bacteria
- RNA modifications and cancer
- Autoimmune and Inflammatory Disorders Research
- Cellular Mechanics and Interactions
- Enzyme Structure and Function
- Antibiotics Pharmacokinetics and Efficacy
- COVID-19 Clinical Research Studies
- Carbon dioxide utilization in catalysis
- biodegradable polymer synthesis and properties
- Systemic Lupus Erythematosus Research
- Ubiquitin and proteasome pathways
- Viral Infections and Immunology Research
- Biosensors and Analytical Detection
- Biochemical and Molecular Research
- RNA regulation and disease
- CRISPR and Genetic Engineering
- ATP Synthase and ATPases Research
- SARS-CoV-2 and COVID-19 Research
- Reproductive System and Pregnancy
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Respiratory viral infections research
- Pneumonia and Respiratory Infections
Second Affiliated Hospital of Fujian Medical University
2016-2025
Fujian Medical University
2016-2025
Guangdong Ocean University
2021-2024
Fudan University
2013-2024
Zhongshan Hospital of Xiamen University
2024
Capital Institute of Pediatrics
2013-2024
Chinese Academy of Agricultural Sciences
2024
Ministry of Agriculture and Rural Affairs
2024
Institute of Food Science and Technology
2024
The Affiliated Yongchuan Hospital of Chongqing Medical University
2023-2024
To control the ongoing coronavirus disease-2019 (COVID-19) pandemic, CoronaVac (Sinovac), an inactivated vaccine, has been granted emergency use authorization by many countries. However, underlying mechanisms of COVID-19 vaccine-induced immune response remain unclear, and little is known about its features compared to (Severe acute respiratory syndrome 2) SARS-CoV-2 infection. Here, we implemented single-cell RNA sequencing (scRNA-seq) profile longitudinally collected PBMCs (peripheral blood...
Community-acquired pneumonia (CAP) is the primary cause of death for children under five years age globally. Hence, it essential to investigate new early biomarkers and potential mechanisms involved in disease severity.Proteomics combined with metabolomics was performed identify suitable diagnosis severe CAP. In training cohort, proteomics were on serum samples obtained from 20 CAPs (S-CAPs), 15 non-severe (NS-CAPs) healthy controls (CONs). verification selected their combinations validated...
Endothelial-mesenchymal transition (EndoMT) is an emerging adaptive process that modulates lymphatic endothelial function to drive aberrant vascularization in the tumour microenvironment (TME); however, molecular determinants govern functional role of EndoMT remain unclear. Here, we show cancer-associated fibroblast (CAF)-derived PAI-1 promoted cells (LECs) cervical squamous cell carcinoma (CSCC).Immunofluorescent staining α-SMA, LYVE-1 and DAPI were examined primary samples obtained from 57...
Unraveling the molecular mechanisms for COVID-19-associated encephalopathy and its immunopathology is crucial developing effective treatments. Here, we utilized single-cell transcriptomic analysis integrated clinical observations laboratory examination to dissect host immune responses reveal pathological in pediatric encephalopathy. We found that lymphopenia was a prominent characteristic of perturbation COVID-19 patients with encephalopathy, especially those acute necrotizing (AE). This...
Mycoplasma pneumoniae (MP) is an important causative agent of morbidity and mortality among all age groups, especially patients extreme ages. Improved readily available tests for accurate, sensitive rapid diagnosis MP infection sorely needed. Here, we developed a CRISPR-Cas12b-based detection platform on the basis recombinase polymerase amplification (RPA) rapid, simple, accurate infection, named MP-RPA-CRISPR. The RPA was employed amplifying community-acquired respiratory distress syndrome...
CoronaVac (Sinovac), an inactivated vaccine for SARS-CoV-2, has been widely used immunization. However, analysis of the underlying molecular mechanisms driving CoronaVac-induced immunity is still limited. Here, we applied a systems biology approach to understand behind adaptive immune response in cohort 50 volunteers immunized with 2 doses CoronaVac. Vaccination led integrated that included several effector arms system including specific IgM/IgG, humoral and other response, as well innate...
Identifying novel risk factors for erectile dysfunction (ED) is crucial developing targeted intervention. This study aimed to investigate the potential impacts of serum trace elements (TEs) concentration on diabetic ED. A retrospective case–control was conducted involving 51 patients with ED and control subjects. Serum levels copper (Cu), zinc (Zn), magnesium (Mg), iron (Fe), calcium (Ca) were measured using inductively coupled plasma-mass spectrometry (ICP-MS). While most selected TEs...
This study focused on exploring the mechanism of transcription factor FOXD3 promoting proliferation, migration, and invasion trophoblast through ELAV1/TLR4 axis. The placenta villi from pregnancy patients with antiphospholipid syndrome (APS) controls were collected, along HTR-8/SVneo cell lines obtained to detect FOXD3, ELAV1, TLR4 expressions using qRT-PCR western blot. interaction ELAV1 mRNA was verified RNA immunoprecipitation. binding detected Chromatin Immunoprecipitation dual...
Background Psychotherapy has demonstrated comparable efficacy to antidepressant medication in the treatment of major depressive disorder. Metabolic alterations MDD state and response have been detected by functional imaging methods, but underlying white matter microstructural changes remain unknown. The goal this study is apply diffusion tensor techniques investigate psychotherapy-specific responses matter. Methods Twenty-one forty-five outpatients diagnosed with depression underwent before...
Abstract The mechanisms underlying the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) remain unclear. In present study, we aimed to identify key biological processes during differentiation. To this end, downloaded three microarray data sets from Gene Expression Omnibus (GEO) database: GSE12266, GSE18043 and GSE37558. Differentially expressed genes (DEGs) were screened using limma package, enrichment analysis was performed. Protein‐protein interaction network...
Systemic lupus erythematosus (SLE) is a complex autoimmune disease with heterogeneous clinical manifestations and the pathogenesis of SLE still unclear. Various omics results have been reported for SLE, but molecular hallmarks especially in patients different activity, using an integrated multi-omics approach not fully investigated. Here, we collected blood samples from 10 healthy controls (HCs) 40 activity including inactive (IA), low (LA), high (HA). Using integrative analysis proteomic,...
The influences of the Li<sup>+</sup>/Ni<sup>2+</sup> replacement modulated by minor Co dopant on cyclic capacity and rate performance lithium-rich cathode material Li<sub>1.2</sub>Ni<sub>0.2−z/2</sub>Mn<sub>0.6−z/2</sub>Co<sub>z</sub>O<sub>2</sub> (<italic>z</italic> = 0, 0.02, 0.04, 0.10) were investigated from microstructural point view comprehensive techniques HRTEM, EELS, SAED, XRD.
Caprin-1 plays roles in many important biological processes, including cellular proliferation, innate immune response, stress response and synaptic plasticity. has been implicated several human diseases, osteosarcoma, breast cancer, viral infection, hearing loss neurodegenerative disorders. The functions of depend on its molecular-interaction network. Direct interactions have established between the fragile X mental retardation protein (FMRP), Ras GAP-activating protein-binding 1 (G3BP1)...
Abstract Background Integrins play a prominent role in osteogenic differentiation by transmitting both mechanical and chemical signals. Integrin expression is closely associated with tensile stress, which has positive effect on differentiation. We investigated the relationship between integrin αVβ3 stress. Methods Human fibroblasts were treated c (RGDyk) lentivirus transduction to inhibit function of αVβ3. Y-15, cytochalasin D verteporfin used phosphorylation FAK, polymerization...
The PLLA/PCL multi-block copolymer was introduced into the stereocomplexed PLA matrix, and its effect on crystallization, thermal mechanical properties of discussed.
−1 programmed ribosomal frameshifting (PRF) is utilized by many viruses to synthesize their enzymatic (Pol) and structural (Gag) proteins at a defined ratio. For efficient PRF, two cis -acting elements are required: heptanucleotide frameshift site downstream stimulator such as pseudoknot. We have analyzed the gag-pol junction sequences from 4254 HIV-1 strains. Approximately ninety-five percent of can form four pseudoknots PK1–PK4 (∼97% contain PK1, PK3, PK4), covering ∼72 nt including site....
BACKGROUND:Acute respiratory distress syndrome (ARDS) in infants is acute and progressive hypoxic failure caused by various extrapulmonary pathogenic factors besides cardiogenic factors. Diffuse alveolar injury progression to pulmonary fibrosis are pathological features of ARDS. The present study sought determine how puerarin influences the inflammatory response ARDS infants. MATERIAL AND METHODS:The human lung fibroblasts cell line HLF1 was treated with different concentrations groups for...
Dibutyl phthalate (DBP) is used worldwide in solvents and plasticizers. The cytotoxicity potential tumorigenic effect of DBP have been reported. has also shown to impact reproductive function. In this study, further evaluate the effects on granulosa cells (GCs), we treated rat GCs vitro with before evaluation biological alterations these GCs. We found that did not induce significant GC death at tested concentrations. However, follicle-stimulating hormone (FSH)-induced KIT ligand (KITLG)...