A5033383665- Obstructive Sleep Apnea Research
- Neuroscience of respiration and sleep
- Tumors and Oncological Cases
- Thyroid Cancer Diagnosis and Treatment
- Tracheal and airway disorders
- Head and Neck Anomalies
- Vascular Malformations and Hemangiomas
- Sleep and related disorders
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Teratomas and Epidermoid Cysts
- Neonatal and fetal brain pathology
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- CRISPR and Genetic Engineering
- Sleep and Wakefulness Research
- Salivary Gland Tumors Diagnosis and Treatment
- Municipal Solid Waste Management
- Respiratory viral infections research
- Sarcoma Diagnosis and Treatment
- Advanced biosensing and bioanalysis techniques
- Cardiovascular and Diving-Related Complications
- Head and Neck Cancer Studies
- Cancer, Hypoxia, and Metabolism
Children's Hospital of Capital Institute of Pediatrics
2020-2025
Peking Union Medical College Hospital
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2022-2025
Capital Institute of Pediatrics
2021-2024
St. Michael's Hospital
2024
University of Toronto
2024
South China Municipal Engineering Design and Research Institute (China)
2019-2023
Beijing University of Chinese Medicine
2023
Woolcock Institute of Medical Research
2023
Shanghai Architectural Design & Research Institute
2019-2022
Coronavirus Disease 2019 (COVID-19), which is caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), has rapidly spread leading to a global pandemic. Here, we combined multiple cross displacement amplification (MCDA) with CRISPR-Cas12a-based detection develop novel diagnostic test (MCCD) and applied for the diagnosis of COVID-19, called COVID-19 MCCD. The MCCD protocol conducts reverse transcription MCDA (RT-MCDA) reaction RNA templates followed CRISPR-Cas12a/CrRNA complex...
Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb) infection, is currently the deadliest infectious disease in human that can evolve to severe forms. A comprehensive immune landscape for Mtb infection critical achieving TB cure, especially patients. We performed single-cell RNA transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing of 213,358 cells from 27 samples, including 6 healthy donors 21 active patients with varying severity (6 mild, moderate 9 cases). Two published...
Background The known risk factors of childhood OSAS include tonsillar and adenoidhypertrophy, obesity, craniofacial anomalies, neuromuscular disorders African-American (AA) ancestry. Whether other such as allergic rhinitis (AR), premature, environmental tobacco smoking (ETS) are associated with inconsistent in different studies. Our study enrolled children a broad age range included potential derived from previous studies our own experience. objective is to identify clinical setting. Methods...
Although previous studies have reported the use of metabolomics for infectious diseases, little is known about potential function plasma metabolites in children infected with Mycoplasma pneumoniae (MP). Here, a combination liquid chromatography-quadrupole time-of-flight mass spectrometry and random forest-based classification model was used to provide broader range applications MP diagnosis. In training cohort, from 63 pneumonia (MPPs), 37 healthy controls (HC) 29 disease (IDC) collected....
To control the ongoing coronavirus disease-2019 (COVID-19) pandemic, CoronaVac (Sinovac), an inactivated vaccine, has been granted emergency use authorization by many countries. However, underlying mechanisms of COVID-19 vaccine-induced immune response remain unclear, and little is known about its features compared to (Severe acute respiratory syndrome 2) SARS-CoV-2 infection. Here, we implemented single-cell RNA sequencing (scRNA-seq) profile longitudinally collected PBMCs (peripheral blood...
Abstract Anisotropic microstructures are widely used by being cleverly designed to achieve important functions. Mammals' respiratory tract is filled with dense cilia that rhythmically swing back and forth in a unidirectional wave propel mucus harmful substances out of the lung through larynx. Inspired ciliary structure motion mechanism systems, viscoelastic microsphere transporting strategy based on integration airway cilium‐like magnetically responsive flexible conical arrays demonstrated....
To explore the neural difference between children with obstructive sleep apnea (OSA) and healthy controls, together relation this cognitive dysfunction of OSA.Twenty OSA (7.2 ± 3.1 years, hypopnea index (AHI): 16.5 16.6 events/h) 29 controls (7.7 2.8 AHI: 1.7 1.2 were recruited matched age, gender, handedness. All underwent resting-state fMRI (rs-fMRI) T1-wighted imaging. Some sedated for MRI scanning. We compared amplitude low frequency fluctuation (ALFF) regional homogeneity (ReHo) those...
Community-acquired pneumonia (CAP) is the primary cause of death for children under five years age globally. Hence, it essential to investigate new early biomarkers and potential mechanisms involved in disease severity.Proteomics combined with metabolomics was performed identify suitable diagnosis severe CAP. In training cohort, proteomics were on serum samples obtained from 20 CAPs (S-CAPs), 15 non-severe (NS-CAPs) healthy controls (CONs). verification selected their combinations validated...
Unraveling the molecular mechanisms for COVID-19-associated encephalopathy and its immunopathology is crucial developing effective treatments. Here, we utilized single-cell transcriptomic analysis integrated clinical observations laboratory examination to dissect host immune responses reveal pathological in pediatric encephalopathy. We found that lymphopenia was a prominent characteristic of perturbation COVID-19 patients with encephalopathy, especially those acute necrotizing (AE). This...
Hepatitis B virus (HBV) is one of the most dangerous and prevalent agents that causes acute chronic liver diseases in humans. Genotyping plays an important role determining clinical outcomes response to antiviral treatment HBV–infected patients. Here, we first devised a CRISPR–based testing platform, termed “CRISPR-HBV,” for ultrasensitive, highly specific, rapid detection two major HBV genotypes (HBV-B HBV-C) application. The CRISPR-HBV employed multiple cross displacement amplification...
CoronaVac (Sinovac), an inactivated vaccine for SARS-CoV-2, has been widely used immunization. However, analysis of the underlying molecular mechanisms driving CoronaVac-induced immunity is still limited. Here, we applied a systems biology approach to understand behind adaptive immune response in cohort 50 volunteers immunized with 2 doses CoronaVac. Vaccination led integrated that included several effector arms system including specific IgM/IgG, humoral and other response, as well innate...
Various studies have highlighted the important associations between obstructive sleep apnea (OSA) and gut microbiota related metabolites. Nevertheless, establishment of causal relationships these remains to be determined. Multiple mendelian randomization (MR) analyses were performed genetically predict causative impact 196 83 metabolites on OSA. Two-sample MR was used assess potential association, causality evaluated using inverse variance weighted (IVW), MR-Egger, median (WM) methods....
Neuroblastoma (NB) is one of the most common extracranial solid tumors in children, which has complex molecular mechanisms. Increasing evidence suggested that long noncoding RNAs (lncRNAs) account for NB pathogenesis. However, function small nucleolar RNA host gene 16 (SNHG16) currently unclear. In present study, publically available data and clinical specimens were employed to verify expression SNHG16 NB. Colony formation, real‑time cell proliferation migration assays performed demonstrate...
Alterations gastrointestinal diseases (GDs) were reported in individuals with obstructive sleep apnea (OSA), however, the genetic background between OSA and GDs is still unclear.
Sleep disturbances are associated with poor health outcomes in children. However, the sleep problems that occur children's growth and metabolism need to be further studied. The aim of present study was assess effects habits on school-aged This cross-sectional included 5832 children adolescents aged 6–18 years from 10 primary secondary schools a district Beijing between 2022 2023. Children's characteristics were evaluated using Habits Questionnaire (CSHQ). Body composition, body mass index...
Streptococcus pyogenes (group A streptococcus, GAS) is the leading bacterial cause of acute pharyngitis in children and adolescents. Rapid reliable diagnosis GAS essential for guiding a timely antibiotic treatment. Here, we developed rapid, highly sensitive, specific test platform detection GAS, designated GAS-MCDA-CRISPR. In this diagnostic platform, multiple cross displacement amplification (MCDA) technique was utilized to preamplify speB gene GAS. Subsequently, CRISPR-Cas12a-based...
Airborne pathogens and antimicrobial resistance (AMR) present significant global health threats. Household waste collection facilities (WCFs), crucial initial nodes in urban management systems, have been understudied regards to their role emitting these hazards. This study investigated the abundance, composition, sources, driving mechanisms, risks associated with AMR originating from WCFs a major city, using culture-based analysis, high-throughput sequencing, risk modeling, respectively. The...
Systemic lupus erythematosus (SLE) is a complex autoimmune disease with heterogeneous clinical manifestations and the pathogenesis of SLE still unclear. Various omics results have been reported for SLE, but molecular hallmarks especially in patients different activity, using an integrated multi-omics approach not fully investigated. Here, we collected blood samples from 10 healthy controls (HCs) 40 activity including inactive (IA), low (LA), high (HA). Using integrative analysis proteomic,...
The objective of this study was to investigate the effectiveness a machine learning algorithm in diagnosing OSA children based on clinical features that can be obtained nonnocturnal and nonmedical environments. This conducted at Beijing Children's Hospital from April 2018 October 2019. participants were 2464 aged 3-18 suspected having who underwent data collection polysomnography(PSG). Participants' randomly divided into training set testing ratio 8:2. elastic net used for feature selection...