A5063114955- DNA Repair Mechanisms
- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Thyroid Cancer Diagnosis and Treatment
- Cancer-related gene regulation
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- PARP inhibition in cancer therapy
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Signaling Pathways in Disease
- Neuroendocrine Tumor Research Advances
- Cancer therapeutics and mechanisms
- Foot and Ankle Surgery
- Hippo pathway signaling and YAP/TAZ
- Sarcoma Diagnosis and Treatment
- Veterinary Orthopedics and Neurology
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Ferroptosis and cancer prognosis
- Craniofacial Disorders and Treatments
- Orthodontics and Dentofacial Orthopedics
- Genetic factors in colorectal cancer
- Bryophyte Studies and Records
Beijing Children’s Hospital
2018-2024
Capital Medical University
2000-2024
Beijing Institute of Genomics
2013-2024
Chinese Academy of Sciences
2013-2024
University of Chinese Academy of Sciences
2016-2023
Beijing Center for Disease Prevention and Control
2022
Beihang University
2020-2021
Columbia University Irving Medical Center
2018-2021
Nanjing Institute of Vegetable Science
2019
University of Hong Kong
2017
5-Hydroxymethylcytosine (5-hmC) may represent a new epigenetic modification of cytosine. While the dynamics 5-hmC during neurodevelopment have recently been reported, little is known about its genomic distribution and function(s) in neurodegenerative diseases such as Huntington's disease (HD). We here observed marked reduction signal YAC128 (yeast artificial chromosome transgene with 128 CAG repeats) HD mouse brain tissues when compared age-matched wild-type (WT) mice, suggesting deficiency...
Neuroblastoma (NB) is the most common malignant tumor originating from extracranial sympathetic nervous system in children. The molecular mechanisms underlying this disease are complex, and not completely understood. Quantitative real-time PCR (qRT-PCR) was applied to quantify expression of miR-20a-5p its target gene ATG7 clinical NB tissues. biological function SH-SY5Y cells investigated through vitro studies (Real-Time cell kinetic analyzer, colony formation assay, caspase-Glo 3/7 assay...
Pediatric tuberculosis (TB) is a serious infectious disease that affects many children worldwide and more likely to be extrapulmonary than adult TB. However, the clinical epidemiological profile, cost burden of pediatric TB (EPTB) in China remain unknown. Here, we conducted descriptive, multicenter study patients from 22 hospitals across all six regions October 2015 December 2018. Of 4,654 patients, 54.23% (2,524) had pulmonary (PTB), 17.76% (827) EPTB, 28.00% (1,303) concurrent (combined...
REV1 is a eukaryotic member of the Y-family DNA polymerases involved in translesion synthesis and genome mutagenesis. Recently, also found to function homologous recombination. However, it remains unclear how recruited sites where recombination processed. Here, we report that loss mammalian results specific defect replication-associated gene conversion. We targeted laser-induced damage stripes manner dependent on its ubiquitin-binding motifs, RAD18, monoubiquitinated FANCD2 (FANCD2-mUb)...
DNA polymerase η (Polη) facilitates translesion synthesis (TLS) across ultraviolet (UV) irradiation- and cisplatin-induced lesions implicated in skin carcinogenesis chemoresistant phenotype formation, respectively. However, whether post-translational modifications of Polη are involved these processes remains largely unknown. Here, we reported that human undergoes O-GlcNAcylation at threonine 457 by O-GlcNAc transferase upon damage. Abrogation this modification results a reduced level...
Neuroblastoma (NB) is one of the most common extracranial solid tumors in children, which has complex molecular mechanisms. Increasing evidence suggested that long noncoding RNAs (lncRNAs) account for NB pathogenesis. However, function small nucleolar RNA host gene 16 (SNHG16) currently unclear. In present study, publically available data and clinical specimens were employed to verify expression SNHG16 NB. Colony formation, real‑time cell proliferation migration assays performed demonstrate...
DNA polymerase κ (Polκ) is the only known Y-family that bypasses 10S (+)-trans-anti-benzo[a]pyrene diol epoxide (BPDE)-N(2)-deoxyguanine adducts efficiently and accurately. The unique features of Polκ, a large structure gap between catalytic core little finger domain 90-residue addition at N terminus as N-clasp, may give rise to its special translesion capability. We designed constructed two mouse Polκ variants, which have reduced size on both sides [Polκ Gap Mutant (PGM) 1] or one side...
Translesion DNA synthesis (TLS) is one mode of damage tolerance that uses specialized polymerases to replicate damaged DNA. polymerase η (Polη) well known facilitate TLS across ultraviolet (UV) irradiation and mutations in POLH are implicated skin carcinogenesis. However, the basis for recruitment Polη stalled replication forks not completely understood. In this study, we used an affinity purification approach isolate a Polη-containing complex have identified SART3, pre-mRNA splicing factor,...
Translesion DNA synthesis (TLS) can use specialized polymerases to insert and/or extend nucleotides across lesions, thereby limiting stalled replication fork collapse and the potential for cell death. Recent studies have shown that monoubiquitinated proliferating nuclear antigen (PCNA) plays an important role in recruitment of Y-family TLS forks after damage treatment. To explore possible roles other factors regulate ultraviolet (UV)-induced assembly at arrested forks, we performed...
Chiyi Jiang, Yeran Yang, Wenfa Xiaoli Ma, Ping Chu, Chao Duan, Xinyu Wang, Binglin Jian, Wen Zhao, Sidou He, Xisi Shihan Zhang, Qian Peiyi Cheng Huang, Geng Yan Su, Yongli Guo
Transcription cofactor vestigial-like 3 (VGLL3), as a master regulator of female-biased autoimmunity, also functions in tumor development, while the underlying mechanisms remain largely elusive. Here, we report that VGLL3 plays an important role DNA damage response (DDR). can be recruited to sites PARylation-dependent manner. depletion impairs accumulation RNF8 and RAD51 at damage, leading reduced homologous recombination efficiency increased cellular sensitivity chemotherapeutic drugs....
Papillary thyroid carcinoma (PTC) is the most common type of carcinoma, and its incidence has been on increase in recent years. However, molecular mechanism PTC unclear misdiagnosis remains a major issue. Therefore, present study aimed to investigate this mechanism, identify key prognostic biomarkers. Integrated analysis was used explore differentially expressed genes (DEGs) between healthy tissue. To functions pathways associated with DEGs, Gene Ontology, pathway protein-protein interaction...
Abstract Background The objective of this study was to investigate the genetic causes two probands diagnosed as Waardenburg syndrome (WS type I and IV) from unrelated Chinese families. Methods PAX3 SOX10 were main pathogenic genes for WS I) IV IV), respectively; all coding exons these sequenced on their family members. Luciferase reporter assay co‐immunoprecipitation (CO‐IP) conducted verify potential functional outcomes novel mutations. Results first proband is a 9 years old girl with I. A...
Abstract High‐risk neuroblastoma (HR‐NB) is an aggressive childhood cancer that responds poorly to currently available therapies and associated with only about a 50% 5‐year survival rate. MYCN amplification critical driver of these tumors, but so far there have not been any approved treatments effectively treat HR‐NB by targeting or its downstream effectors. Thus, the identification novel molecular targets therapeutic strategies children diagnosed represents urgent unmet medical need. Here,...
Abstract Background Neuroblastoma (NB) is the most common extracranial malignant solid tumor in children, which highly prone to bone marrow (BM) metastasis. BM can monitor early signs of mild disease and Existing biomarkers are insufficient for diagnosis treatment NB. Bromodomain PHD finger transcription factor (BPTF) an important subunit chromatin-remodeling complex that closely associated with tumors. Here, we evaluated whether BPTF plays role predicting NB progression, explore molecular...
Increasing evidence suggests that long non-coding RNAs (lncRNAs) are involved in neuroblastoma (NB) pathogenesis. The aim of this study was to elucidate the roles and underlying mechanism RNA activated by DNA damage (NORAD) childhood NB. Both public data clinical specimens were used determine NORAD expression. Colony formation, cell proliferation wound healing assays performed evaluate effects on migration SH-SY5Y SK-N-BE(2) cells. Flow cytometry examine cycle changes. expression genes...
Hepatoblastoma (HB), a leading primary hepatic malignancy in children, originates from primitive stem cells. This study aimed to uncover the genetic variants that are responsible for HB oncogenesis. One family, which includes healthy parents, and two brothers affected by HB, was recruited. Whole-genome sequencing (WGS) of germline DNA all family members identified maternal variants, located within APC gene X-linked WAS gene, were harbored brothers. The mutation (rs137854573, c.C1606T,...
ABSTRACT Introduction Cardiac neoplasms are particularly rare in children, and the majority of these tumors benign. Approximately 10% cardiac malignant, including soft tissue sarcomas lymphomas. could also be metastases. Primitive EWSR1‐ negative round or spindle cell undifferentiated sarcoma harboring CIC gene translocation is a highly aggressive malignancy mainly occurring tissues. However, it has not yet been described heart. Case presentation We report that arose from right ventricle...
The effect of different types canine guidance on the patterns laterotrusive tracing at incisal point and relationship between inclinations working side condylar movements were investigated in 42 young subjects. subjects divided into M D groups according to their mesial distal guidance, also protrusive laterotrusion (PL) retrusive (RL) patterns. No differences found groups. pattern had no corresponding association with type guidance. In relation condyles, significant PL RL condyles moved...