A5023083036- Tea Polyphenols and Effects
- DNA Repair Mechanisms
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Phytochemicals and Antioxidant Activities
- Food Quality and Safety Studies
- Ubiquitin and proteasome pathways
- RNA Research and Splicing
- Hepatitis B Virus Studies
- Metabolomics and Mass Spectrometry Studies
- Advanced biosensing and bioanalysis techniques
- Mass Spectrometry Techniques and Applications
- GABA and Rice Research
- Hepatitis C virus research
- Food composition and properties
- Cytomegalovirus and herpesvirus research
- Fermentation and Sensory Analysis
- Bacteriophages and microbial interactions
Anhui Agricultural University
2024
Beijing Institute of Genomics
2016-2024
Chinese Academy of Sciences
2016-2024
University of Chinese Academy of Sciences
2017-2018
Abstract Tea seedlings (Camellia sinensis) have a well-developed root system with strong taproot and lateral roots. Compared ordinary cuttings, tea has stronger vitality environmental adaptability, thus facilitating the promotion of good varieties. However, there is less detailed research on rooting germination process seeds. In this study, matrix-assisted laser desorption ionization time-of-flight–mass spectrometry was used to conduct non-targeted spatial mass imaging main organs during...
DNA polymerase η (Polη) facilitates translesion synthesis (TLS) across ultraviolet (UV) irradiation- and cisplatin-induced lesions implicated in skin carcinogenesis chemoresistant phenotype formation, respectively. However, whether post-translational modifications of Polη are involved these processes remains largely unknown. Here, we reported that human undergoes O-GlcNAcylation at threonine 457 by O-GlcNAc transferase upon damage. Abrogation this modification results a reduced level...
Translesion DNA synthesis (TLS) is one mode of damage tolerance that uses specialized polymerases to replicate damaged DNA. polymerase η (Polη) well known facilitate TLS across ultraviolet (UV) irradiation and mutations in POLH are implicated skin carcinogenesis. However, the basis for recruitment Polη stalled replication forks not completely understood. In this study, we used an affinity purification approach isolate a Polη-containing complex have identified SART3, pre-mRNA splicing factor,...
RAD18, an important ubiquitin E3 ligase, plays a dual role in translesion DNA synthesis (TLS) and homologous recombination (HR) repair. However, whether how the regulatory mechanism of O-linked N-acetylglucosamine (O-GlcNAc) modification governing RAD18 its function during these processes remains unknown. Here, we report that human can undergo O-GlcNAcylation at Ser130/Ser164/Thr468, which is for optimal accumulation damage sites. Mechanistically, abrogation limits CDC7-dependent Ser434...
Abstract Next generation sequencing (NGS) technologies have dramatically improved studies in biology and biomedical science. However, no optimal NGS approach is available to conveniently analyze low frequency mutations caused by DNA damage treatments. Here, developing an exquisite ultra-sensitive (USNGS) platform “EasyMF” incorporating it with a widely used supF shuttle vector-based mutagenesis system, we can dissect roles of lesion bypass polymerases damage-induced mutagenesis. In this...
Objective To express and purify active HBV RNase H in vitro candidate for drug screening. Methods Codon-optimized cDNA sequences genotype C was cloned by gene synthesis into pMAL-c5xHis with a N-terminal MBP tag C-terminal hexahistidine tag. expressed E. coli BL21 cells. proteins were enriched nickel-agarose affinity chromatography identified through SDS-PAGE gel electrophoresis Western blot. An oligonucleotide-directed RNA cleavage assay used to measure the activity, stability...