Human monkeypox (MPX) is a severe and reemerging infectious disease caused by virus (MPXV) forms two distinct lineages, including Congo Basin West African clades. Due to the absence of specific vaccines antiviral drugs, developing point-of-care (POC) testing system identify MPXV critical for preventing controlling MPX transmission. Here, CRISPR/Cas12b diagnostic platform was integrated with loop-mediated isothermal amplification (LAMP) devise novel CRISPR-MPXV approach ultrasensitive, highly...

Abstract Objectives In the current study, for first time, we reported a novel HCV molecular diagnostic approach termed reverse transcription loop-mediated isothermal amplification integrated with gold nanoparticles-based lateral flow biosensor (RT-LAMP-AuNPs-LFB), which developed rapid, sensitive, specific, simple, and visual identification of HCV. Methods A set LAMP primer was designed according to 5’untranslated region (5’UTR) gene from major genotypes 1b, 2a, 3b, 6a, 3a, are prevalent in...

Hepatitis B virus (HBV) is one of the most dangerous and prevalent agents that causes acute chronic liver diseases in humans. Genotyping plays an important role determining clinical outcomes response to antiviral treatment HBV–infected patients. Here, we first devised a CRISPR–based testing platform, termed “CRISPR-HBV,” for ultrasensitive, highly specific, rapid detection two major HBV genotypes (HBV-B HBV-C) application. The CRISPR-HBV employed multiple cross displacement amplification...

Tuberculosis (TB) is a chronic infectious disease caused by the etiological agent Mycobacterium tuberculosis (MTB). Because majority of TB patients come from poor economic backgrounds, development simple, specific, low-cost, and highly sensitive detection method for pathogen extremely important prevention treatment this disease. In current study, an efficient visual, rapid, MTB utilizing multiplex loop-mediated isothermal amplification combined with label-based lateral flow immunoassay...

Abstract Human mpox is a zoonotic disease, similar to smallpox, caused by the virus, which further subdivided into Congo Basin and West African clades with different pathogenicity. In this study, novel diagnostic protocol utilizing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR‐associated 12a nuclease (CRISPR/Cas12a)‐mediated recombinase polymerase amplification (RPA) was developed identify in Africa (CRISPR‐RPA). Specific RPA primers targeting D14L ATI were...

Tuberculosis (TB) is a chronic infectious disease with high mortality caused by the Mycobacterium tuberculosis complex (MTC). Its clinical symptoms include prolonged cough mucus, pleuritic chest pain, hemoptysis, etc., and predominant complications such as tuberculous meningitis pleural effusion. Thus, developing rapid, ultrasensitive, highly specific detection techniques plays an important role in controlling TB. Here, we devised CRISPR/CRISPR-associated 12b nuclease (CRISPR/Cas12b)-based...

ABSTRACT Brucellosis is caused by members of the Brucella spp. and remains one world’s major zoonotic diseases. melitensis ( B. ) as most contagious species cannot be ignored an essential source infection for brucellosis, especially in countries/regions dominated animal husbandry. Thus, identification genus crucial rapid diagnosis brucellosis to control disease transmission clinical treatment. Here, we developed CRISPR/Cas12b nuclease combined with a multiple cross displacement amplification...

ABSTRACT Infection with hepatitis B virus (HBV) and C (HCV) is a major contributor to liver-related morbidity mortality worldwide. An accurate rapid point-of-care (POC) diagnostic approach the gateway for effective treatment control of these infections. Here, first time, we integrated isothermal multiple cross-displacement amplification (MCDA) gold nanoparticle-based lateral flow biosensor (AuNPs-LFB) successfully develop novel HBV&HCV-MCDA-AuNPs-LFB assay simultaneous accurate,...

Abstract Epstein-Barr virus (EBV) is a ubiquitous gamma herpesvirus that maintains lifelong latent association with B lymphocytes. Here, rapid and reliable diagnosis platform for detecting EBV infection, employing loop-mediated isothermal amplification (LAMP) combined gold nanoparticles–based lateral flow biosensors (AuNPs-LFB) (termed LAMP Amplification Mediated AuNPs-LFB Detection, LAMAD), was developed in the current study. A set of specific primers targeting nuclear antigen (EBNA) leader...

Abstract Hepatitis C remains a global health problem, especially in poverty‐stricken areas. A rapid and sensitive point‐of‐care (POC) diagnostic tool is critical for the early detection timely treatment of hepatitis virus (HCV) infection. Here, first time, we reported novel molecular assay, termed reverse transcription multiple cross displacement amplification integrated with gold‐nanoparticle‐based lateral flow biosensor (RT‐MCDA‐AuNPs‐LFB), which was developed rapid, sensitive, specific,...

Tuberculosis (TB) is a chronic infectious disease mainly caused by Mycobacterium tuberculosis (MTB), but other members of the complex (MTBC), especially bovis (pyrazinamide-resistant organisms), may also be involved. Thus, ability to rapidly detect and identify MTB from MTBC (e.g., M. , microti africanum ) essential for prevention treatment TB. A novel diagnostic method rapid detection differentiation MTB, which employs multiplex loop-mediated isothermal amplification (mLAMP) combined with...

Introduction Brucellosis was made statutorily notifiable in 1955, China, while Guizhou Province, the pathogen of human brucellosis isolated for first time 2011. However, currently, epidemic is becoming more and severe Province. The type distribution genetic characteristics Brucella as well its evolutionary relationship with domestic foreign strains, are still unclear. Methods MLST, MLVA, rpoB typing techniques were used molecular epidemiological study 83 isolates province. Results Among MLST...

Abstract Background Tuberculosis (TB) is a serious chronic infectious disease caused by Mycobacterium tuberculosis complex (MTBC). Hence, the development of novel, simple, rapid and sensitive method to detect MTBC great significance for prevention treatment TB. Results In this study, multiple cross displacement amplification (MCDA) combined with nanoparticle-based lateral flow biosensor (LFB) was developed simultaneously two target genes ( IS6110 mpb64 ) (MCDA-LFB). One suite specific MCDA...

Brucella abortus (B. abortus) as an important infectious agent of bovine brucellosis cannot be ignored, especially in countries/regions dominated by animal husbandry. Thus, the development ultrasensitive and highly specific identification technique is ideal strategy to control transmission brucellosis. In this report, a novel detection protocol, which utilizes multiple cross displacement amplification (MCDA) combined with gold nanoparticles-based lateral flow biosensor (AuNPs-LFB) targeting...

Leprosy is an ancient disease caused by Mycobacterium leprae (ML) that remains a public health problem in poverty-stricken areas worldwide. Although many ML detection techniques have been used, rapid and sensitive tool essential for the early treatment of leprosy. Herein, we developed technique combining multiple cross displacement amplification (MCDA) with nanoparticle-based lateral flow biosensor (LFB), termed ML-MCDA-LFB. MCDA induced isothermal reaction using specific primers targeting...

Coronavirus disease 2019 (COVID-19) is a highly infectious caused by severe acute respiratory syndrome coronavirus 2 (SARS-COV-2). Though many methods have been used for detecting SARS-COV-2, development of an ultrafast and sensitive detection strategy to screen and/or diagnose suspected cases in the population, especially early-stage patients with low viral load, significant prevention treatment COVID-19. In this study, novel restriction endonuclease-mediated reverse transcription multiple...

Epstein-Barr virus (EBV), a double-stranded DNA belonging to the family Herpesviridae, infects more than 95% of healthy adults by attacking host immune system. Here, novel detection protocol, utilizing modified multiple cross displacement amplification (MCDA) technique combined with gold nanoparticles-based lateral flow biosensors (AuNPs-LFB), was devised and developed detect EBV infection (termed EBV-MCDA-LFB assay). Ten MCDA primers targeting EBNA-LP gene were designed, including CP1*...

Abstract Background Mycobacterium leprae (ML) is the pathogen that causes leprosy, which has a long history and still exists today. ML an intracellular mycobacterium dominantly induces leprosy by causing permanent damage to skin, nerves, limbs eyes as well deformities disabilities. Moreover, grows slowly nonculturable in vitro. Given prevalence of highly sensitive rapid method for early diagnosis urgently needed. Results In this study, we devised novel tool combining restriction...

Viral hepatitis caused by B virus (HBV) and C (HCV) infections remain a major global public health challenge, particularly in low- middle-income countries. It is crucial to utilize pointof-care (POC) testing platform that sensitive, specific, rapid, user-friendly for screening diagnosis of the two infections. Here, novel molecular diagnostic assay, integrating multiplex loop-mediated isothermal amplification with gold nanoparticle-based lateral flow biosensor (mLAMP-AuNPs-LFB) was developed...

African swine fever (ASF) is a devastating disease that can kill almost all infected pigs, causing great damage to the pig industry and destabilizing global economy. Here, we developed specific assay combined multiple cross-displacement amplification (MCDA) with nanoparticle-based lateral flow biosensor (LFB) for early rapid identification of virus (ASFV-MCDA-LFB). We first designed set MCDA primers recognize 10 different regions target ASFV B646L gene. Subsequently, reaction was monitored...

Abstract Background: Mycobacterium tuberculosis complex (MTBC) causes (TB), which is a global public health problem that seriously endangers health. Hence, development of new and rapid method to detect MTBC great significance for prevention treatment TB. Results : In this study, multiple cross displacement amplification combined with nanoparticle-based lateral flow biosensor (MCDA-LFB) was developed simultaneously two target genes ( IS6110 mpb64 ) MTBC. One suit specific (MCDA) primers,...