A5103163157- RNA Interference and Gene Delivery
- Plant-Microbe Interactions and Immunity
- Advanced biosensing and bioanalysis techniques
- Weed Control and Herbicide Applications
- Immunotherapy and Immune Responses
- Crystallization and Solubility Studies
- Nanoplatforms for cancer theranostics
- X-ray Diffraction in Crystallography
- Catalytic Processes in Materials Science
- Advanced Photocatalysis Techniques
- Traditional Chinese Medicine Analysis
- Pesticide and Herbicide Environmental Studies
- Phytochemistry and Biological Activities
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Plant Disease Resistance and Genetics
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- DNA and Nucleic Acid Chemistry
- Legume Nitrogen Fixing Symbiosis
- Immune cells in cancer
- Cytomegalovirus and herpesvirus research
- Plant Pathogens and Fungal Diseases
- Fungal Plant Pathogen Control
- Peanut Plant Research Studies
Sun Yat-sen University
2014-2025
Jilin University
2021-2025
The University of Texas Health Science Center at Houston
2024
Wuhan University
2016-2024
Liaoning Academy of Agricultural Sciences
2014-2023
Institute of Plant Protection
2014-2023
Jiamusi University
2023
First Affiliated Hospital of Jiamusi University
2023
Central South University
2006-2022
Nankai University
2019-2022
Coronavirus Disease 2019 (COVID-19), which is caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), has rapidly spread leading to a global pandemic. Here, we combined multiple cross displacement amplification (MCDA) with CRISPR-Cas12a-based detection develop novel diagnostic test (MCCD) and applied for the diagnosis of COVID-19, called COVID-19 MCCD. The MCCD protocol conducts reverse transcription MCDA (RT-MCDA) reaction RNA templates followed CRISPR-Cas12a/CrRNA complex...
An electrochemical N2 reduction reaction (NRR), as an environmentally benign method to produce NH3, is a suitable alternative substitute the energy-intensive Haber–Bosch technology. Unfortunately, date, it obstructed by lack of efficient electrocatalysts. Here, CoS2/MoS2 nanocomposite with CoS2 nanoparticles decorated on MoS2 nanosheets fabricated and adapted catalyst for NRR. As unveiled experimental theoretical results, strong interaction between modulates interfacial charge distribution...
Abstract Hypoxia‐induced resistance to tumor treatment restricts further development of photodynamic therapy. Instead simple reoxygenation relieve hypoxia in traditional therapeutic approaches, a mitochondria‐targeted reactive oxygen species (ROS) amplifier is constructed reverse and enhance sensitivity hypoxia‐resistant Mesoporous silica nanoparticles are modified with triphenylphosphine its blood circulation endow it specificity. α‐Tocopherol succinate indocyanine green loaded mesoporous...
DNA-based nanoprobes have attracted extensive interest in the field of bioanalysis. Notably, engineered DNA that can respond to multiple pathological parameters are desirable detect targets precisely. Here we design a split aptamer/DNAzyme (aptazyme)-based probe for fluorescence detection ATP and further develop cooperatively activatable nanoprobe tumor-specific imaging vivo. The comprising aptazyme-coated MnO2 nanovectors high stability synergistically activated by biomarkers, GSH ATP. Upon...
Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) is a promising target for herbicide discovery. Search new compounds with novel chemotypes key objective agrochemists. Here, we describe the discovery and systematic SAR-based structure optimization of N-isoxazolinylphenyltriazinones 5-9 as PPO inhibitors. The in vivo herbicidal activity vitroNicotiana tabacum (NtPPO) inhibitory were explored detail. A number synthetic displayed strong Ki values nanomolar range. Some exhibited excellent...
An azide and hydrazine tethered to a naphthalimide analogue was created selectively react with 5-formyluracil in one system fluorogenically label 5-formylcytosine another system.
In continuation of our search for potent protoporphyrinogen IX oxidase (PPO, EC 1.3.3.4) inhibitors, we designed and synthesized a series novel herbicidal cycloalka[d]quinazoline-2,4-dione-benzoxazinones. The bioassay results these compounds indicated that most the exhibited very strong Nicotiana tabacum PPO (NtPPO) inhibition activity. More than half 37 displayed over 80% control all three tested broadleaf weeds at 37.5-150 g ai/ha by postemergent application, majority them showed no...
Unmethylated cytosine-phosphate-guanosine (CpG) oligodeoxynucleotides are immunostimulatory nucleic acids wildly utilized as adjuvants or for vaccines to treat diseases. However, there is a lack of simple and efficient vectors CpG oligodeoxynucleotide delivery with long-lasting immune stimulation. Herein, self-assembled polymer wires consisting motifs by hybridization chain reaction were constructed excellent biocompatibility activity. The designed DNA acted programmable multivalent...
Blockade of A2A adenosine receptors (A2AR)-adenosinergic signaling shows high potency to mobilize antitumor immunity for its in-depth involvement in immune regulation nearly all cells. Available A2AR inhibition strategies are mainly based on small molecules or proteins inhibitors, yet limited by the non-specific operation as well off-target toxicity. Herein, first effort design a convenient tumor-specific strategy improve responses via spatiotemporally controlled oxygen supply virtue...
Photodynamic therapy (PDT) utilizes reactive oxygen species (ROS) to treat established diseases and has attracted growing attention in the field of cancer therapy. However, a tumor microenvironment (TME), inherent hypoxia high level antioxidants severely hamper efficacy ROS generation. Here, we describe cascaded amplifier nanoreactor based on self-assembled nanofusiforms for persistent oxygenation amplify levels. The nanofusiform assembly is capable photothermal photodynamic treatment...
Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) is an effective target for green herbicide discovery. In this work, we reported the unexpected discovery of a novel series N-phenylaminomethylthioacetylpyrimidine-2,4-diones (2–6) as promising PPO inhibitors based on investigating reaction intermediates our initially designed N-phenyluracil thiazolidinone (1). An efficient one-pot procedure that gave 41 compounds in good to high yields was developed. Systematic Nicotiana tabacum (NtPPO) inhibitory...
A key objective for herbicide research is to develop new compounds with improved bioactivity. Protoporphyrinogen IX oxidase (PPO) an essential target discovery. Here, we report using in silico structure-guided optimization approach of our previous lead compound 1 and designed synthesized a series 2-6. Systematic bioassays led the discovery highly potent 6g, 1-methyl-3-(2,2,7-trifluoro-3-oxo-4-(prop-2-yn-1-yl)-3,4-dihydro-2H-benzo[b][1,4]oxazin-6-yl)thieno[2,3-d]pyrimidine-2,4(1H,3H)-dione,...
Abstract The development of potent strigolactone (SL) agonists as suicidal germination inducers could be a useful strategy for controlling root parasitic weeds, but uncertainty about the SL perception mechanism impedes real progress. Here we describe small-molecule that efficiently stimulate Phelipanchce aegyptiaca , and Striga hermonthica in concentrations low 10 −8 to −17 M. We show full efficiency synthetic triggering signaling through receptor, ShHTL7, depends on receptor-catalyzed...
Effective adjuvants that enhance immune responses have great potential for vaccine development and disease prevention. A major challenge is the effective delivery of component to specific cellular populations in lymph nodes boost response. Here, immunostimulatory DNA nanogel designed self-assembled with applicable size lymphatic drainage high efficacy. The efficient node accumulation, enhanced dendritic cell internalization, sustained stimulus such adjuvant formulation enable potent...
Protoporphyrinogen oxidase (PPO, EC 1.3.3.4) is an important target for green agrochemical discovery. Herein, a novel N-phenylisoxazoline-thiadiazolo[3,4-a]pyridazine herbicidal active scaffold was designed by the hybridization strategy. Systematic structural optimization enabled discovery of series derivatives with excellent weed control at 9.375–150 g ai/ha post-emergent application. Some exhibited improved Nicotiana tabacum PPO (NtPPO)-inhibitory activity than fluthiacet-methyl. Of these,...
DNA amplifier circuits establish powerful tools to dynamically control molecular assembly for computation, sensing, and biological applications. However, the slow reaction speed remains a major barrier their practical utility. Here, diverse fast termed toehold exchange polymerization (TEP) catalysis (TEC) using exchange-mediated as fundamental mechanism are built. Both TEP TEC with duplex hairpin can respond within minutes nucleic acid inputs high fidelity. In addition, amplify live-cell...
Increased intracellular reactive oxygen species (ROS) are involved in doxorubicin (DOX)-induced myocardial cell apoptosis, and paeoniflorin (PEF) has been shown to exert an antioxidant effect. The aim of the present study was explore protective effect PEF on DOX-induced apoptosis underlying mechanisms. In cultured H9c2 cells, different concentrations (1, 10, or 100 μmol/L) added for 2 h prior exposure DOX (5 24 h. Cell evaluated by hoechst 33342 staining, caspase-3 expression activity. mRNA...
Summary Background IL‐21 is a key cytokine for regulating B cell immunity, which involved in several inflammatory conditions. This study sought to define role activated lymphocytes and enhanced tissue eosinophilia NP tissues during the pathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). Methods uncinate process were collected from 64 CRSwNP patients, 25 CRSsNP 29 control subjects. expression was examined using IHC staining, qRT‐PCR, flow cytometry, ELISA, its clinical...