A5085437167- Plant Parasitism and Resistance
- Plant and animal studies
- Plant biochemistry and biosynthesis
- Plant Molecular Biology Research
- Legume Nitrogen Fixing Symbiosis
- Insect-Plant Interactions and Control
- Sesquiterpenes and Asteraceae Studies
- Plant tissue culture and regeneration
- Microbial Natural Products and Biosynthesis
- Allelopathy and phytotoxic interactions
- Agronomic Practices and Intercropping Systems
- Plant Gene Expression Analysis
- Biochemical and biochemical processes
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Pharmacological Effects of Natural Compounds
- Plant Pathogens and Fungal Diseases
- Seed Germination and Physiology
- Plant Virus Research Studies
- Natural product bioactivities and synthesis
- Insect and Pesticide Research
- Plant Toxicity and Pharmacological Properties
- Microbial Metabolic Engineering and Bioproduction
- Essential Oils and Antimicrobial Activity
- Greenhouse Technology and Climate Control
University of Amsterdam
2017-2025
Wageningen University & Research
2014-2023
International Rice Research Institute
2023
Université de Rennes
2023
Kansas State University
2023
University of Arizona
2023
Netherlands Institute for Neuroscience
2018-2022
Graduate School Experimental Plant Sciences
2008-2019
Centre for BioSystems Genomics
2000-2015
King Abdullah University of Science and Technology
2015
Strigolactones, phytohormones with diverse signaling activities, have a common structure consisting of two lactones connected by an enol-ether bridge. Strigolactones derive from carotenoids via pathway involving the carotenoid cleavage dioxygenases 7 and 8 (CCD7 CCD8) iron-binding protein D27. We show that D27 is β-carotene isomerase converts all-trans-β-carotene into 9-cis-β-carotene, which cleaved CCD7 9-cis-configured aldehyde. CCD8 incorporates three oxygens 9-cis-β-apo-10'-carotenal...
The seeds of parasitic plants the genera Striga and Orobanche will only germinate after induction by a chemical signal exuded from roots their host. Up to now, several these germination stimulants have been isolated identified in root exudates series host both spp. In most cases, compounds were shown be isoprenoid belong one class, collectively called strigolactones, suggested many authors sesquiterpene lactones. However, this classification was never proven; hence, biosynthetic pathways are...
Abstract In this study, the role of recently identified class phytohormones, strigolactones, in shaping root architecture was addressed. Primary lengths strigolactone-deficient and -insensitive Arabidopsis (Arabidopsis thaliana) plants were shorter than those wild-type plants. This accompanied by a reduction meristem cell number, which could be rescued application synthetic strigolactone analog GR24 all genotypes except strigolactone-insensitive mutant. Upon treatment, cells transition zone...
Volatile components, such as terpenoids, are emitted from aerial parts of plants and play a major role in the interaction between their environment. Analysis composition emission pattern volatiles model plant Arabidopsis showed that range volatile components released, primarily flowers. Most detected were monoterpenes sesquiterpenes, which contrast to other diurnal pattern. The active terpenoid metabolism wild-type provoked us conduct an additional set experiments transgenic overexpressing...
Herbivore-damaged plants release complex mixtures of volatiles that attract natural enemies the herbivore. To study relevance individual components these for predator attraction, we manipulated herbivory-induced through genetic engineering. Metabolic engineering terpenoids, which dominate composition many induced plant volatile bouquets, holds particular promise. By switching subcellular localization introduced sesquiterpene synthase to mitochondria, obtained transgenic Arabidopsis thaliana...
Fruit flavor is a result of complex mixture numerous compounds. The formation these compounds closely correlated with the metabolic changes occurring during fruit maturation. Here, we describe use DNA microarrays and appropriate statistical analyses to dissect developmental process. In doing so, have identified novel strawberry alcohol acyltransferase (SAAT) gene that plays crucial role in biogenesis ripening fruit. Volatile esters are quantitatively qualitatively most important providing...
The biosynthesis of the recently identified novel class plant hormones, strigolactones, is up-regulated upon phosphate deficiency in many species. It generally accepted that evolutionary origin strigolactone up-regulation their function as a rhizosphere signal stimulates hyphal branching arbuscular mycorrhizal fungi. In this work, we demonstrate induction conserved Arabidopsis (Arabidopsis thaliana), although not host for We increase production contributes to changes shoot architecture...
The blends of flavor compounds produced by fruits serve as biological perfumes used to attract living creatures, including humans. They include hundreds metabolites and vary in their characteristic fruit composition. molecular mechanisms which aroma are gained lost during evolution domestication largely unknown. Here, we report on processes that may have been responsible for the diversity strawberry (Fragaria spp) components. Whereas terpenoid profile cultivated species is dominated...
DOI: 10.1111/j.1469-8137.2008.02463.x Commentary p 695
The (seco)iridoids and their derivatives, the monoterpenoid indole alkaloids (MIAs), form two large families of plant-derived bioactive compounds with a wide spectrum high-value pharmacological insect-repellent activities. Vinblastine vincristine, MIAs used as anticancer drugs, are produced by Catharanthus roseus in extremely low levels, leading to high market prices poor availability. Their biotechnological production is hampered fragmentary knowledge biosynthesis. Here we report discovery...
Summary 1 The paper reviews the literature on seed dormancy, with special regard to incon sistencies in terms and definitions used. It presents a concept of dormancy which physiology ecology are integrated. Its aim is increase understanding germination, help defining ecological research questions. 2 claimed that should not be identified absence germination. Seed rather defined as characteristic, degree determines range conditions able germinate. Dormancy varies continuous scale, visualized...
Cucurbitacins are triterpenoids that confer a bitter taste in cucurbits such as cucumber, melon, watermelon, squash, and pumpkin. These compounds discourage most pests on the plant have also been shown to antitumor properties. With genomics biochemistry, we identified nine cucumber genes pathway for biosynthesis of cucurbitacin C elucidated four catalytic steps. We discovered transcription factors Bl ( Bitter leaf ) Bt fruit regulate this leaves fruits, respectively. Traces genomic...
Abstract Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins considered to be factor response regulating gene expression after symbiotic signaling. However, legume NSP1 can functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP2, indicating that both conserved in higher plants. Here, we show indispensable strigolactone (SL) biosynthesis the...
Inventors in the field of mechanical and electronic engineering can access multitudes components and, thanks to standardization, parts from different manufacturers be used combination with each other. The introduction BioBrick standards for assembly characterized DNA sequences was a landmark microbial engineering, shaping synthetic biology. Here, we describe standard Type IIS restriction endonuclease-mediated assembly, defining common syntax 12 fusion sites enable facile eukaryotic...