A5031841376- Plant Molecular Biology Research
- Light effects on plants
- Gene Regulatory Network Analysis
- Photosynthetic Processes and Mechanisms
- Circadian rhythm and melatonin
- Bacterial Genetics and Biotechnology
- Plant Pathogens and Fungal Diseases
- Salivary Gland Disorders and Functions
- Plant Physiology and Cultivation Studies
- Plant and Biological Electrophysiology Studies
- Seedling growth and survival studies
- Plant Reproductive Biology
- Photoreceptor and optogenetics research
- Plant Pathogens and Resistance
- Growth and nutrition in plants
- Cell Image Analysis Techniques
- Plant nutrient uptake and metabolism
- Silicon Effects in Agriculture
- Plant Surface Properties and Treatments
- Composting and Vermicomposting Techniques
- Greenhouse Technology and Climate Control
- Animal Genetics and Reproduction
- Plant Gene Expression Analysis
- Seed Germination and Physiology
- Insect Pest Control Strategies
University of Cambridge
2016-2025
Sainsbury Laboratory
2016-2025
Microsoft Research (United Kingdom)
2016-2020
Newcastle University
2009-2020
Norwich Research Park
2019
Agricultural Research Service
2006-2018
Hebrew University of Jerusalem
2018
NIHR Newcastle Biomedical Research Centre
2015-2018
John Innes Centre
2015
Howard Hughes Medical Institute
2011-2013
Plants are responsive to temperature, and some species can distinguish differences of 1°C. In Arabidopsis, warmer temperature accelerates flowering increases elongation growth (thermomorphogenesis). However, the mechanisms perception largely unknown. We describe a major thermosensory role for phytochromes (red light receptors) during night. Phytochrome null plants display constitutive warm-temperature response, consistent with this, we show in this background that transcriptome becomes...
Abstract Circadian clocks maintain robust and accurate timing over a broad range of physiological temperatures, characteristic termed temperature compensation. In Arabidopsis thaliana, ambient affects the rhythmic accumulation transcripts encoding clock components TIMING OF CAB EXPRESSION1 (TOC1), GIGANTEA (GI), partially redundant genes CIRCADIAN CLOCK ASSOCIATED1 (CCA1) LATE ELONGATED HYPOCOTYL (LHY). The amplitude peak levels increase for TOC1 GI RNA rhythms as increases (from 17 to...
Inventors in the field of mechanical and electronic engineering can access multitudes components and, thanks to standardization, parts from different manufacturers be used combination with each other. The introduction BioBrick standards for assembly characterized DNA sequences was a landmark microbial engineering, shaping synthetic biology. Here, we describe standard Type IIS restriction endonuclease-mediated assembly, defining common syntax 12 fusion sites enable facile eukaryotic...
Significance Plants generate organs throughout their life as a consequence of the maintenance postembryonic stem cell niches in meristems. The molecular mechanisms controlling homeostasis and organ emergence shoot meristems have been well described, but manner which environmental signals influence them to plasticity is largely unknown. Using apical meristem Arabidopsis model system, we show that plants can adapt organogenesis rate changes availability nitrate soil within few days, thanks...
Abstract Temperature compensation contributes to the accuracy of biological timing by preventing circadian rhythms from running more quickly at high than low temperatures. We previously identified quantitative trait loci (QTL) with temperature-specific effects on rhythm leaf movement, including a QTL linked transcription factor FLOWERING LOCUS C (FLC). have now analyzed FLC alleles in near-isogenic lines and induced mutants eliminate other candidate genes. showed that lengthened period...
Gene regulatory circuits can use dynamic, and even stochastic, strategies to respond environmental conditions. We examined activation of the general stress response mediated by alternative sigma factor, σ(B), in individual Bacillus subtilis cells. observed that energy activates σ(B) discrete stochastic pulses, with increasing levels leading higher pulse frequencies. By perturbing rewiring endogenous system, we found this behavior results from three key features circuit: an ultrasensitive...
Background Fatigue is a debilitating condition with significant impact on patients' quality of life. frequently reported by patients suffering from primary Sjögren's Syndrome (pSS), chronic autoimmune characterised dryness the eyes and mouth. However, although fatigue common in pSS, it does not manifest all sufferers, providing an excellent model which to explore potential underpinning biological mechanisms. Methods Whole blood samples 133 fully-phenotyped pSS stratified for presence...
Cells use general stress response pathways to activate diverse target genes in a variety of stresses. However, responses coexist with more specific that are activated by individual stresses, provoking the fundamental question whether and how cells control generality or specificity their particular stress. Here we address this issue using quantitative time-lapse microscopy Bacillus subtilis environmental response, mediated σ(B). We analyzed σ(B) activation stresses such as salt ethanol...
To assess the diagnostic and behavioural overlap of headache patients with temporomandibular disorders (TMD), individuals recruited from general population self-described headaches were compared non-headache controls. The examination procedures in Research Diagnostic Criteria (RDC) for TMD applied to both sets subjects by a blinded examiner. Following their examination, used experience sampling methods obtain data on pain, tooth contact, masticatory muscle tension, emotional states stress....
The Arabidopsis circadian clock orchestrates gene regulation across the day/night cycle. Although a multiple feedback loop circuit has been shown to generate 24-hr rhythm, it remains unclear how robust is in individual cells, or timing coordinated plant. Here we examine activity at single cell level seedlings over several days under constant environmental conditions. Our data reveal oscillations, albeit desynchronised. In particular, observe two waves of activity; one going down, and up...
Gene expression can be noisy, as the growth of single cells. Such cell-to-cell variation has been implicated in survival strategies for bacterial populations. However, it remains unclear how cells couple gene with to implement these strategies. Here, we show noisy a key stress-response regulator, RpoS, allows E. coli modulate its dynamics survive future adverse environments. We reveal dynamic positive feedback loop between RpoS and rate that produces multi-generation pulses. do so...
Multicellular development produces patterns of specialized cell types. Yet, it is often unclear how individual cells within a field identical initiate the patterning process. Using live imaging, quantitative image analyses and modeling, we show that during Arabidopsis thaliana sepal development, fluctuations in concentration transcription factor ATML1 pattern epidermal to differentiate into giant interspersed between smaller cells. We find expressed all However, its level fluctuates each...
Several commercial formulations of botanical extracts and essential oils are being investigated as possible alternatives to soil fumigation for control Fusarium wilt diseases. Soil infested with oxysporum f. sp. chrysanthemi was treated 1, 5, 10% aqueous emulsions formulated clove (70% oil), neem (90% pepper/mustard (chili pepper extract oil mustard), cassia (extract tree), Banrot (a standard fungicide applied at different labeled rates) in separate experiments. Population densities F. were...
There are very few reference solutions in the literature on non-Boussinesq natural convection flows. We propose here a test case problem which extends well-known De Vahl Davis differentially heated square cavity to of large temperature differences for Boussinesq approximation is no longer valid. The paper split two parts: this first part, we as yet unpublished cases characterized by non-dimensional difference 0.6, Ra = 106 (constant property and variable cases) 107 (variable case). These...
Abstract Objective Rheumatoid arthritis (RA) is considered to be a prototypical autoimmune disease. However, the autoantigens that play an important role in development of RA remain unclear. The aim this study was investigate whether T cells specific for citrullinated epitopes from self proteins are present patients with RA. Methods Peripheral blood mononuclear (PBMCs) 28 and 18 healthy controls were stimulated or noncitrullinated aggrecan peptide Agg 84–103 , proliferative cytokine...