A5049504767- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Plant responses to elevated CO2
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Plant Water Relations and Carbon Dynamics
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Light effects on plants
- Plant tissue culture and regeneration
- Rice Cultivation and Yield Improvement
- Chromosomal and Genetic Variations
- Genetic Mapping and Diversity in Plants and Animals
- Mitochondrial Function and Pathology
- CRISPR and Genetic Engineering
- Legume Nitrogen Fixing Symbiosis
- Microbial Metabolic Engineering and Bioproduction
- GABA and Rice Research
- Plant Genetic and Mutation Studies
- Algal biology and biofuel production
- Agronomic Practices and Intercropping Systems
- Plant Gene Expression Analysis
- Plant Taxonomy and Phylogenetics
- Plant Micronutrient Interactions and Effects
University of Sheffield
2016-2025
International Rice Research Institute
2015-2025
Chinese Academy of Agricultural Sciences
2020-2024
Biotechnology Research Institute
2020-2024
ARC Centre of Excellence for Transformative Meta-Optical Systems
2015-2022
Australian National University
2015-2022
Institute of Apiculture Research
2022
International Crops Research Institute for the Semi-Arid Tropics
2020
Rothamsted Research
2004
AgResearch
2004
Summary Much of humanity relies on rice ( Oryza sativa ) as a food source, but cultivation is water intensive and the crop vulnerable to drought high temperatures. Under climate change, periods reduced availability temperature are expected become more frequent, leading detrimental effects yields. We engineered high‐yielding cultivar ‘ IR 64’ produce fewer stomata by manipulating level developmental signal. overexpressed epidermal patterning factor Os EPF 1 , creating plants with...
Another "green revolution" is needed for crop yields to meet demands food. The international C(4) Rice Consortium working toward introducing a higher-capacity photosynthetic mechanism--the pathway--into rice increase yield. goal identify the genes necessary install photosynthesis in through different approaches, including genomic and transcriptional sequence comparisons mutant screening.
Inventors in the field of mechanical and electronic engineering can access multitudes components and, thanks to standardization, parts from different manufacturers be used combination with each other. The introduction BioBrick standards for assembly characterized DNA sequences was a landmark microbial engineering, shaping synthetic biology. Here, we describe standard Type IIS restriction endonuclease-mediated assembly, defining common syntax 12 fusion sites enable facile eukaryotic...
Abstract. The effect of gradually-developing water-stress has been studied in Lupinus albus L., Helianthus annuus Vitis vinifera cv. Rosaki and Eucalyptus globulus Labill. Water was withheld diurnal rhythms were investigated 4–8d later, when the predawn water deficit more negative than watered plants, stomata closed almost completely early during photoperiod. contribution 'stomatal' 'non-stomatal' components to decrease photosynthetic rate by (1) comparing changes photosynthesis air with...
C i , intercellular CO 2 concentration F v / m quantum efficiency of excitation capture by open photosystem II centres FBPase, fructose‐1,6‐bisphosphatase GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase GDC, glycine decarboxylase GS‐2, chloroplastic glutamine synthetase HPR, hydroxypyruvate reductase PFD, photon flux density Φ assimilation PSII, electron transport ψ water potential q N non‐photochemical chlorophyll a fluorescence quenching P photochemical RuBP, ribulose‐1,5‐bisphosphate...
Models describing the evolution of partial pressure atmospheric oxygen over Phanerozoic time are constrained by mass balances required between inputs and outputs carbon sulfur to oceans. This constraint has limited applicability proposed negative feedback mechanisms for maintaining levels O 2 at biologically permissable levels. Here we describe a modeling approach that incorporates -dependent isotope fractionation using data obtained from laboratory experiments on carbon-13 discrimination...
The C4 photosynthetic pathway accounts for ∼25% of primary productivity on the planet despite being used by only 3% species. Because plants are higher yielding than C3 plants, efforts underway to introduce into crop rice. This is an ambitious endeavor; however, evolved from multiple independent occasions over last 30 million years, and steps along trajectory evident in extant One approach toward engineering rice recapitulate this trajectory, one first which was a change leaf anatomy....
A pair of GOLDEN2-LIKE transcription factors is required for normal chloroplast development in land plant species that encompass the range from bryophytes to angiosperms. In C(4) maize, compartmentalized function two GLK genes bundle sheath and mesophyll cells regulates dimorphic differentiation, whereas C(3) plants Physcomitrella patens Arabidopsis thaliana act redundantly all photosynthetic cells. To assess whether cell-specific unique we analyzed gene expression patterns monocot Sorghum...
Summary Photosynthetic induction describes the transient increase in leaf CO 2 uptake with an light. During induction, efficiency is lower than at steady state. Under field conditions of fluctuating light, this during may cost > 20% potential crop assimilation. Accelerating would boost photosynthetic and resource‐use efficiencies. Variation between rice accessions for accelerating was analysed by gas exchange. Induction shade to sun transitions 14 representing five subpopulations from...
A fundamental tenet of multicellular eukaryotic evolution is that vertical inheritance paramount, with natural selection acting on genetic variants transferred from parents to offspring. This lineal process means an organism’s adaptive potential can be restricted by its evolutionary history, the amount standing variation, and mutation rate. Lateral gene transfer (LGT) theoretically provides a mechanism bypass many these limitations, but importance frequency this in eukaryotes, such as...
The development and maturation of the CRISPR/Cas genome editing system provides a valuable tool for plant functional genomics genetic improvement. Currently available genome-editing tools have limited number targets, restricting their application in research. In this study, we developed novel CRISPR/Cas9 ultra-multiplex consisting two template vectors, eight donor four destination one primer-design software package. By combining advantages Golden Gate cloning to assemble multiple repetitive...
Light intensity and atmospheric CO2 partial pressure are two environmental signals known to regulate stomatal numbers. It has previously been shown that if a mature Arabidopsis leaf is supplied with either elevated (750 ppm instead of ambient at 370 ppm) or reduced light levels (50 μmol m−2 s−1 250 s−1), the young, developing leaves not receiving treatment grow density as they were exposed treatment. But signal(s) it believed generated in transmitted largely unknown. Photosynthetic rates...
• Stomatal density responses by 48 accessions of Arabidopsis, to CO2 enrichment, broadly parallel interspecific observations. Accessions differing in the degree stomatal response both and drought differed flower production. Under well watered conditions flowering benefits from a small reduction with but large under drought. increases altitude Vaccinium myrtillus is also strongly influenced exposure. Exposed plants had higher densities than at same community individuals. This difference might...
Abstract Suc-phosphate synthase (SPS) is a key regulatory enzyme in the pathway of Suc biosynthesis and has been linked to quantitative trait loci controlling plant growth yield. In dicotyledonous plants there are three SPS gene families: A, B, C. Here we report finding five families genes wheat (Triticum aestivum) other monocotyledonous from family Poaceae (grasses). Three these form separate subfamilies within previously described C families, but two novel distinctive D family, which on...
Cells associated with veins of petioles C(3) tobacco possess high activities the decarboxylase enzymes required in C(4) photosynthesis. It is not clear whether this case other species, nor these provide precursors for specific biosynthetic pathways. Here, we investigate activity acid decarboxylases mid-vein Arabidopsis, identify regulatory regions sufficient activity, and determine impact removing individual isoforms each protein on metabolite profiles. This showed that radiolabelled malate...
To boost food production for a rapidly growing global population, crop yields must significantly increase. One of the avenues being recently explored is improvement photosynthetic capacity by installing C4 pathway into C3 crops like rice to drastically increase their yield. Crops with an enhanced mechanism would better utilize solar radiation that can be translated This subsequently will help in producing more grain yield, reduce water loss and nitrogen use efficiency especially hot dry...
Several tools are available to identify miRNAs from deep-sequencing data, however, only a few of them, like miRDeep, can novel and also as standalone application. Given the difference between plant animal miRNAs, particularly in terms distribution hairpin length nature complementarity with its duplex partner (or miRNA star), underlying (statistical) features miRDeep other tools, using similar features, likely get affected. The potential effects on such minimum free energy, stability...