A5051914912- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Genomics and Chromatin Dynamics
- Single-cell and spatial transcriptomics
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Nuclear Structure and Function
- Ubiquitin and proteasome pathways
- Photosynthetic Processes and Mechanisms
- RNA Research and Splicing
- Land Use and Ecosystem Services
- Plant-Microbe Interactions and Immunity
- Genetic Mapping and Diversity in Plants and Animals
- Anodic Oxide Films and Nanostructures
- Extracellular vesicles in disease
- Plant Pathogens and Resistance
- Advanced Proteomics Techniques and Applications
- Agronomic Practices and Intercropping Systems
- Legume Nitrogen Fixing Symbiosis
- Tissue Engineering and Regenerative Medicine
- Light effects on plants
- Plant Micronutrient Interactions and Effects
- Plant and Biological Electrophysiology Studies
Humboldt-Universität zu Berlin
2017-2024
Humboldt State University
2022
Wageningen University & Research
2010-2019
University of Potsdam
2017
MRC Laboratory of Molecular Biology
2013-2014
Graduate School Experimental Plant Sciences
2013
State Key Laboratory of Molecular Biology
2013
Max Planck Institute for Molecular Genetics
2013
Centre for BioSystems Genomics
2009-2012
Floral organs are specified by the combinatorial action of MADS-domain transcription factors, yet mechanisms which proteins activate or repress expression their target genes and nature cofactors still largely unknown. Here, we show using affinity purification mass spectrometry that five major floral homeotic (AP1, AP3, PI, AG, SEP3) interact in tissues as proposed “floral quartet” model. In vitro studies confirmed a flexible composition protein complexes depending on relative concentrations...
The molecular mechanisms by which floral homeotic genes act as major developmental switches to specify the identity of organs are still largely unknown. Floral encode transcription factors MADS-box family, supposed assemble in a combinatorial fashion into organ-specific multimeric protein complexes. Major mediators interactions MADS-domain proteins SEPALLATA subfamily, play crucial role development all types organs. In order characterize roles SEPALLATA3 factor complexes at level, we...
Zinc is an essential micronutrient for all living organisms. When facing a shortage in zinc supply, plants adapt by enhancing the uptake capacity. The molecular regulators controlling this adaptation are not known. We present identification of two closely related members Arabidopsis thaliana basic-region leucine-zipper ( bZIP ) transcription factor gene family, bZIP19 and bZIP23 , that regulate to low supply. They were identified, yeast-one-hybrid screening, associate promoter regions...
Abstract Legume GRAS (GAI, RGA, SCR)-type transcription factors NODULATION SIGNALING PATHWAY1 (NSP1) and NSP2 are essential for rhizobium Nod factor-induced nodulation. Both proteins considered to be factor response regulating gene expression after symbiotic signaling. However, legume NSP1 can functionally replaced by nonlegume orthologs, including rice (Oryza sativa) NSP2, indicating that both conserved in higher plants. Here, we show indispensable strigolactone (SL) biosynthesis the...
Summary The growth‐regulating factors ( GRF s) are plant‐specific transcription factors. They form complexes with ‐interacting GIF s), a small family of transcriptional co‐activators. In Arabidopsis thaliana, seven out the nine s controlled by micro RNA miR396. Analysis plants carrying 3 allele insensitive to miR396 revealed strong boost in number cells leaves, which was further enhanced synergistically an additional increase 1 levels. Genetic experiments that can still cell gif1 mutants,...
Spatial organization of chromatin contributes to gene regulation many cellular processes and includes a connection with the nuclear lamina (NL). The NL is protein mesh that resides underneath inner membrane consists lamins lamina-associated proteins. Chromatin regions associated in animals are characterized mostly by constitutive heterochromatin, but association facultative heterochromatin mediated Polycomb-group (PcG) proteins has been reported as well. In contrast animals, plant components...
Abstract The MADS transcription factors (TF) are an ancient eukaryotic protein family. In plants, the family is divided into two main lineages. Here, we demonstrate that DNA binding in both lineages absolutely requires a short amino acid sequence C-terminal to domain (M domain) called Intervening (I was previously defined only type II lineage MADS. Structural elucidation of MI domains from floral regulator, SEPALLATA3 (SEP3), shows conserved fold with I acting stabilise M domain. Using organ...
Floral organ identities in plants are specified by the combinatorial action of homeotic master regulatory transcription factors. However, how these factors achieve their specificities is still largely unclear. Genome-wide vivo DNA binding data show that MADS domain proteins recognize partly distinct genomic regions, suggesting specificity contributes to functional differences protein complexes. We used vitro systematic evolution ligands exponential enrichment followed high-throughput...
SUMMARY Single‐cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure nucleus isolation combined with nanowell‐based library preparation. Our method enables the transcriptome analysis of thousands individual nuclei. It serves as an alternative to use protoplast isolation, which is currently standard methodology single‐cell genomics, although it can be challenging some tissues. We show applicability...
The formation of the female gamete is a complex developmental process that begins with differentiation Megaspore Mother Cell (MMC) within ovule. SPOROCYTELESS/NOZZLE (SPL/NZZ) principal regulator MMC formation, as mutations in SPL/NZZ gene leads to failure differentiation. Nonetheless, SPL/NZZ-dependent regulatory pathway governing development remains largely unknown. Using multi-omics approach, we identify direct targets and their downstream network. We discovered interacts ovule-identity...
Abstract Plant MADS-domain transcription factors act as key regulators of many developmental processes. Despite the wealth information that exists about these factors, mechanisms by which they recognize their cognate DNA-binding site, called CArG-box (consensus CCW6GG), and how different proteins achieve specificity, are still largely unknown. We used from in vivo ChIP-seq experiments, vitro data evolutionary conservation to address important questions. found structural characteristics DNA...
SUMMARY Single-cell genomics provides unprecedented potential for research on plant development and environmental responses. Here, we introduce a generic procedure nuclei isolation combined with nanowell-based library preparation. Our method enables the transcriptome analysis of thousands individual nuclei. It serves as alternative to use protoplast isolation, which is currently standard methodology single-cell genomics, although it can be challenging some tissues. We show applicability our...
Abstract Plant responses to stress caused by high temperatures involve changes occurring at the molecular, metabolic, and physiological levels. Understanding mechanisms which plants recognize signals activate this response is a prerequisite for identifying key genes signaling pathways obtaining heat-tolerant plants. We demonstrated first implementation of an assay transposase-accessible chromatin identify open regions (OCRs) in crown tissues barley using three genotypes carrying different...
ABSTRACT Identity and functions of plant cells are influenced by their precise cellular location within the body. Cellular heterogeneity in growth differentiation trajectories results organ patterning. Therefore, assessing this at molecular scale is a major question developmental biology. Single-cell transcriptomics (scRNA-seq) allows to characterize quantify gene expression developing organs unprecedented resolution. However, original physical cell lost during scRNA-seq procedure. To...