A5079231709- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- Legume Nitrogen Fixing Symbiosis
- Chromosomal and Genetic Variations
- Light effects on plants
- Seed Germination and Physiology
- Glycosylation and Glycoproteins Research
- Plant Stress Responses and Tolerance
- RNA modifications and cancer
- Plant biochemistry and biosynthesis
- Genetic Syndromes and Imprinting
- CRISPR and Genetic Engineering
- Ubiquitin and proteasome pathways
- Plant Disease Resistance and Genetics
- Plant responses to water stress
- Epigenetics and DNA Methylation
- Plant Virus Research Studies
- Mesoporous Materials and Catalysis
- Genetic Mapping and Diversity in Plants and Animals
- Fern and Epiphyte Biology
Wageningen University & Research
2016-2025
University of Zurich
2012-2021
Graduate School Experimental Plant Sciences
2019
Radboud University Nijmegen
2008-2012
University of Leeds
2010
Research International (United States)
2010
Centre for BioSystems Genomics
2010
Medical University of Vienna
2009
Tomato (Solanum lycopersicum) contains two close homologs of the Arabidopsis thaliana MADS domain transcription factor FRUITFULL (FUL), FUL1 (previously called TDR4) and FUL2 MBP7). Both proteins interact with ripening regulator RIPENING INHIBITOR (RIN) are expressed during fruit ripening. To elucidate their function in tomato, we characterized single double knockdown lines. Whereas lines only showed very mild alterations pigmentation, silenced exhibited an orange-ripe phenotype due to...
The family of small auxin up-regulated RNA (SAUR) genes is a auxin-responsive with ~60–140 members in most higher plant species. Despite the early discovery their responsiveness, function and mode action remained unknown for long time. In recent years, importance SAUR regulation dynamic adaptive growth, molecular mechanisms by which proteins act are increasingly well understood. SAURs play central role auxin-induced acid but can also independently auxin, tissue specifically regulated various...
Monocarpic plants have a single reproductive cycle in their lives, where life span is determined by the coordinated arrest of all meristems, or global proliferative (GPA). The molecular bases for GPA and signaling mechanisms involved are poorly understood, other than systemic cues from developing seeds unknown nature. Here we uncover genetic pathway regulating Arabidopsis that responds to age-dependent factors acts parallel seed-derived signals. We show FRUITFULL (FUL), MADS-box gene...
MADS-domain transcription factors are well known for their roles in plant development and regulate sets of downstream genes that have been uncovered by high-throughput analyses. A considerable number these targets predicted to function hormone responses or environmental stimuli, suggesting there is a close link between developmental regulators growth development. Here, we show the Arabidopsis factor FRUITFULL (FUL) executes several functions addition its noted role fruit Among direct FUL,...
Small Auxin-Upregulated RNA (SAUR) genes encode growth regulators that induce cell elongation. Arabidopsis contains more than 70 SAUR genes, of which the growth-promoting function has been unveiled in seedlings, while their role other tissues remained largely unknown. Here, we focus on regulatory regions to predict processes they play a role, and understand dynamics plant growth. In this study, characterized detail entire SAUR10-clade: SAUR8, SAUR9, SAUR10, SAUR12, SAUR16, SAUR50, SAUR51...
MADS box genes in plants consist of MIKC-type and type I genes. While have been studied extensively, the functions are still poorly understood. Evidence suggests that involved embryo sac seed development. We investigated two independent T-DNA insertion alleles Arabidopsis thaliana gene AGAMOUS-LIKE61 (AGL61) showed agl61 mutant ovules, polar nuclei do not fuse central cell morphology is aberrant. Furthermore, begins to degenerate before fertilization takes place. Although pollen tubes...
Members of the plant type I MADS domain subfamily have been reported to be involved in reproductive development Arabidopsis (Arabidopsis thaliana). However, from 61 genes genome, only PHERES1, AGAMOUS-LIKE80 (AGL80), DIANA, AGL62, and AGL23 functionally characterized, which revealed important roles for these during female gametophyte early seed development. The functions other are still unknown, despite fact that available single T-DNA insertion mutants largely investigated. lack mutant...
Genomic imprinting results in monoallelic gene expression a parent-of-origin-dependent manner and is regulated by the differential epigenetic marking of parental alleles. In plants, genomic has been primarily described for genes expressed endosperm, tissue nourishing developing embryo that does not contribute to next generation. Arabidopsis, MEDEA (MEA) PHERES1 (PHE1), which are imprinted also embryo; whether their embryonic or not, however, remains controversial. contrast, maternally 1...
The timing of flowering and the inflorescence architecture are critical for reproductive success tomato (Solanum lycopersicum), but gene regulatory networks underlying these traits have not been fully explored. Here, we show that FRUITFULL-like (FUL-like) genes FUL2 MADS-BOX PROTEIN 20 (MBP20) promote vegetative-to-reproductive transition repress branching by inducing floral meristem (FM) maturation. FUL1 fulfils a less prominent role appears to depend on MBP20 its upregulation in...
Transformation resulted in deletions and translocations at T-DNA inserts, but not genome-wide small mutations. A tiny splinter was detected that probably would remain undetected by conventional techniques. We investigated to which extent Agrobacterium tumefaciens-mediated transformation is mutagenic, on top of inserting T-DNA. To prevent mutations due vitro propagation, we applied floral dip Arabidopsis thaliana. re-sequenced the genomes five primary transformants, compared these genomic...
Shoot determinacy is a key trait affecting productivity in tomato, quantitatively governed by genes within the flowering pathway. Achieving an optimal balance of signals essential for shaping plant architecture and maximizing yield potential. However, genetic resources allelic diversity available fine-tuning this remain limited. In work, we demonstrate potential directly manipulating shoot simultaneously targeting activating FRUITFULL(FUL)-like genes, FUL2 MADS-BOX PROTEIN 20 (MBP20),...
Establishing the embryonic body plan of multicellular organisms relies on precisely orchestrated cell divisions coupled with pattern formation, which, in animals, are regulated by Polycomb group (PcG) proteins. The conserved Repressive Complex 2 (PRC2) mediates H3K27 trimethylation and comes different flavors Arabidopsis. PRC2 catalytic subunit MEDEA is required for seed development; however, a role patterning has been dismissed. Here, we demonstrate that embryos derived from medea eggs...
Efficient seed dispersal in flowering plants is enabled by the development of fruits, which can be either dehiscent or indehiscent. Dehiscent fruits open at maturity to shatter seeds, while indehiscent do not and seeds are dispersed various ways. The diversity fruit morphology shattering mechanisms enormous within plants. How these different types develop molecular networks driving diversification still largely unknown, despite progress eudicot model species. orchid family, known for its...
Embryonic development requires a correct balancing of maternal and paternal genetic information. This balance is mediated by genomic imprinting, an epigenetic mechanism that leads to parent-of-origin-dependent gene expression. The parental conflict (or kinship) theory proposes imprinting can evolve due between alleles over resource allocation during seed development. One assumption this regulate growth; however, effects on size are often very low or non-existent. We demonstrate there pool...
The MADS-box transcription factor family has expanded considerably in plants via gene and genome duplications can be subdivided into type I MIKC-type genes. two classes show a different evolutionary history. Whereas the genes originated during ancient duplications, as well more recent events, loci appear to experience high turnover with many duplications. This mode of origin also suggests fate for duplicates, which are thought have higher chance become silenced or lost from genome. To get...
The moment at which a plant transitions to reproductive development is paramount its life cycle and strictly controlled by many genes. transcription factor SUPPRESSOR OF OVEREXPRESSION CONSTANS 1 (SOC1) plays central role in this process Arabidopsis. However, the of SOC1 tomato (Solanum lycopersicum) has been sparsely studied. Here, we investigated function four homologs floral transition inflorescence development. We thoroughly characterized SOC1-like clade throughout Solanaceae selected...
Evolution has long been considered to be a conservative process in which new genes arise from pre-existing through gene duplication, domain shuffling, horizontal transfer, overprinting, retrotransposition, etc. However, this view is changing as originating non-genic sequences are discovered different organisms. Still, rather limited functional information available. Here, we have identified TWISTED1 (TWT1), possible de novo-originated protein-coding that modifies microtubule arrangement and...