A5049975254- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Light effects on plants
- Islanding Detection in Power Systems
- Agriculture and Biological Studies
- Ubiquitin and proteasome pathways
- Plant Physiology and Cultivation Studies
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- RNA modifications and cancer
- HVDC Systems and Fault Protection
- Bone Metabolism and Diseases
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
University of Zurich
2020-2025
John Innes Centre
2016-2020
Norwich Research Park
2019
University of Milan
2012-2014
Aarhus University
2013
Hormones, such as auxin and cytokinin, are involved in the complex molecular network that regulates coordinated development of plant organs. Genes controlling ovule patterning have been identified studied detail; however, roles cytokinin largely unknown. Here we show key pathway genes, isopentenyltransferase receptors, expressed during development. Also, a cre1-12 ahk2-2 ahk3-3 triple mutant with severely reduced perception, expression efflux facilitator PIN-FORMED 1 (PIN1) was reduced. In...
Abstract Background MADS-domain transcription factors play important roles during plant development. The Arabidopsis MADS-box gene SHORT VEGETATIVE PHASE ( SVP ) is a key regulator of two developmental phases. It functions as repressor the floral transition vegetative phase and later it contributes to specification meristems. How these distinct activities are conferred by single factor unclear, but interactions with other MADS domain proteins which specify binding different genomic regions...
Tissue patterning in multicellular organisms is the output of precise spatio-temporal regulation gene expression coupled with changes hormone dynamics. In plants, auxin regulates growth and development at every stage a plant's life cycle. Auxin signaling occurs through binding molecule to TIR1/AFB F-box ubiquitin ligase, allowing interaction Aux/IAA transcriptional repressor proteins. These are subsequently ubiquitinated degraded via 26S proteasome, leading derepression response factors...
Upon hormonal signaling, ovules develop as lateral organs from the placenta. Ovule numbers ultimately determine number of seeds that develop, and thereby contribute to final seed yield in crop plants. We demonstrate here CUP-SHAPED COTYLEDON 1 (CUC1), CUC2 AINTEGUMENTA (ANT) have additive effects on ovule primordia formation. show expression CUC1 genes is required redundantly regulate PINFORMED1 (PIN1), which turn for Furthermore, our results suggest auxin response factor MONOPTEROS...
The phytohormone auxin governs crucial developmental decisions throughout the plant life cycle. Auxin signaling is effectuated by response factors (ARFs) whose activity repressed Aux/IAA proteins under low levels, but relieved from repression when cellular concentrations increase. ARF3/ETTIN (ETT) a conserved noncanonical Arabidopsis thaliana ARF that adopts an alternative auxin-sensing mode of translating levels into multiple transcriptional outcomes. However, mechanistic model for how this...
Specification of new organs from transit amplifying cells is critical for higher eukaryote development. In plants, a central stem cell pool maintained by the pluripotency factor SHOOTMERISTEMLESS (STM), surrounded competent to respond auxin hormone maxima giving rise organs. Auxin triggers flower initiation through Response Factor (ARF) MONOPTEROS (MP) and recruitment chromatin remodelers activate genes promoting floral fate. The contribution gene repression reproductive primordium poorly...
ABSTRACT mRNA methylation at the N6-position of adenosine (m6A) enables multiple layers post-transcriptional gene control, often via RNA-binding proteins that use a YT521-B homology (YTH) domain for specific m6A recognition. In Arabidopsis, normal leaf morphogenesis and rate formation require YTH-domain ECT2, ECT3 ECT4. this study, we show ect2/ect3 ect2/ect3/ect4 mutants also exhibit slow root stem growth, flower formation, defective directionality aberrant fruit morphology. all cases,...
In multicellular organisms, sexual reproduction relies on the formation of highly differentiated cells, gametes, which await fertilization in a quiescent state. Upon fertilization, cell cycle resumes. Successful development requires that male and female gametes are same phase cycle. The molecular mechanisms reinstate division fertilization-dependent manner poorly understood both animals plants. Using
BASIC PENTACYSTEINE (BPC) transcription factors have been identified in a large variety of plant species. In Arabidopsis thaliana there are seven BPC genes, which, except for BPC5, expressed ubiquitously. genes functionally redundant wide range developmental processes. Recently, we reported that BPC1 binds to guanine and adenine (GA)–rich consensus sequences the SEEDSTICK (STK) promoter vitro induces conformational changes. Here show by chromatin immunoprecipitation experiments vivo BPCs...
The BASIC PENTACYSTEINE (BCP) family is a poorly characterized plant transcription factor of GAGA BINDING PROTEINS. In Arabidopsis, there are seven members (BPC1-7) that broadly expressed, and they can potentially bind more than 3000 Arabidopsis GAGA-repeat-containing genes. To date, BPCs known to be direct regulators the INNER NO OUTER (INO), SEEDSTICK (STK), LEAFY COTYLEDON 2 (LEC2) Because high functional redundancy, neither single knockout nor double bpc mutant combinations cause...
In multicellular organisms such as animals and plants, development requires the precise regulation of gene expression, mediated not only by transcription factors but also chromatin-based mechanisms. Among these, histone modifications like H3K4me3 H3K27me3 play opposing roles in activation repression, respectively. Arabidopsis thaliana, is deposited Polycomb Repressive Complex 2 (PRC2), while Trithorax group (TrxG) proteins mediate deposition. While functions these writer complexes have been...
ABSTRACT Organ formation in multicellular organisms depends on the coordinated activities of regulatory components that integrate developmental and hormonal cues to control gene expression mediate cell-type specification. For example, development Arabidopsis gynoecium is tightly controlled by distribution synthesis plant hormone auxin. The functions several transcription factors (TFs) have been linked with auxin dynamics during development; yet how their are not known. Here, we show five...
The plant hormone auxin regulates numerous aspects of the life cycle. Auxin signalling is mediated by response factors (ARFs) that dimerise with modulating Aux/IAA repressors. ARF3 (ETTIN or ETT) atypical as it does not interact It proposed to be a non-canonical sensor, regulating diverse functions essential for development. This sensing ability relies on unique C-terminal ETT specific domain (ES domain). Alignments orthologues across angiosperm phylum revealed length and sequence identities...
Establishing the embryonic body plan of multicellular organisms relies on precisely orchestrated cell divisions coupled with pattern formation, which, in animals, are regulated by Polycomb group (PcG) proteins. The conserved Repressive Complex 2 (PRC2) mediates H3K27 trimethylation and comes different flavors Arabidopsis. PRC2 catalytic subunit MEDEA is required for seed development; however, a role patterning has been dismissed. Here, we demonstrate that embryos derived from medea eggs...
Abstract The gynoecium is critical for the reproduction of flowering plants as it contains ovules and tissues that foster pollen germination, growth, guidance. These tissues, known reproductive tract (ReT), comprise stigma, style, transmitting (TT). ReT originate from carpel margin meristem (CMM) within pistil. SHOOT MERISTEMLESS (STM) a key transcription factor formation maintenance. In all above-ground meristems, including CMM, local STM downregulation required organ formation. However,...
Abstract Background The genome-wide profiling of chromatin states that are defined by different histone post-translational modifications, known as epigenomic profiling, is crucial for understanding the epigenetic regulations gene expression, both in animal and plant systems. CUT&Tag (Cleavage Under Targets Tagmentation, [1]) a novel enzyme-tethering method initially developed mammalian cells. has several advantages compared to most commonly used methods such Chromatin Immunoprecipitation...
Abstract Functional diversification of transcription factors and their downstream targets contributes to the emergence new organ morphologies. To better understand molecular mechanisms underlying floral specification in rice, we investigated function OsMADS2 OsMADS4 , rice PISTILLATA ( PI ) paralogs controlling lodicule stamen formation. The morphological characterization osmads2 null mutants generated by CRISPR/Cas9 reiterated OsMADS2’s nonredundant role development. Furthermore, it...
Abstract In multicellular organisms, sexual reproduction relies on the formation of highly specialized, differentiated cells, gametes. At maturity, male and female gametes are quiescent, awaiting fertilization, with their cell cycle being arrested at a precise stage. Failure to establish quiescence leads unwanted proliferation, abortion offspring, waste resources. Upon resumes, allowing newly formed zygote divide rapidly. Successful development requires that in same phase cycle. The...
Abstract The gynoecium is critical for the reproduction of flowering species as it contains ovules and tissues required pollen germination guidance. These are collectively known reproductive tract (ReT) comprise stigma, style transmitting (TT). ReT originate from a meristem within pistil named carpel margin (CMM). SHOOT MERISTEMLESS (STM) key transcription factor formation maintenance. In all above-ground meristems, including CMM, STM has to be locally downregulated allow proper organ...