A5014760681- Redox biology and oxidative stress
- Plant biochemistry and biosynthesis
- Selenium in Biological Systems
- Mycotoxins in Agriculture and Food
- Glutathione Transferases and Polymorphisms
- Phytochemistry and Biological Activities
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Advanced Proteomics Techniques and Applications
- Photosynthetic Processes and Mechanisms
- Genomics, phytochemicals, and oxidative stress
- X-ray Diffraction in Crystallography
- Plant and animal studies
- Natural product bioactivities and synthesis
- Plant chemical constituents analysis
- Plant tissue culture and regeneration
- Crystallization and Solubility Studies
- Endoplasmic Reticulum Stress and Disease
- Free Radicals and Antioxidants
- Mitochondrial Function and Pathology
- Trace Elements in Health
- Phytochemical compounds biological activities
- Organoselenium and organotellurium chemistry
- Weed Control and Herbicide Applications
- Plant and fungal interactions
Institut de Biologie Moléculaire des Plantes
2004-2023
Centre National de la Recherche Scientifique
2016-2023
Université de Strasbourg
2014-2023
Technical University of Denmark
2010-2018
Interactions Arbres-Microorganismes
2004-2008
Université de Lorraine
2004-2008
Swedish University of Agricultural Sciences
2008
Centre Hospitalier Régional et Universitaire de Nancy
2006
Ecologie et Ecophysiologie Forestières
2004
The (seco)iridoids and their derivatives, the monoterpenoid indole alkaloids (MIAs), form two large families of plant-derived bioactive compounds with a wide spectrum high-value pharmacological insect-repellent activities. Vinblastine vincristine, MIAs used as anticancer drugs, are produced by Catharanthus roseus in extremely low levels, leading to high market prices poor availability. Their biotechnological production is hampered fragmentary knowledge biosynthesis. Here we report discovery...
In living cells, reactive oxygen species (ROS) play a key role in signaling but these compounds can also damage macromolecules. As other compartments, the mitochondrial ROS concentrations need to be tightly controlled. Plant mitochondria contain several antioxidant systems that are not only able scavenge and limit their production repair damages macromolecules possibly serve as redox sensors. They comprise ascorbate‐ glutathione‐dependent pathways well based on thioredoxin (TRX)‐...
We provide here an exhaustive overview of the glutathione (GSH) peroxidase (Gpx) family poplar (Populus trichocarpa). Although these proteins were initially defined as GSH dependent, in fact they use only reduced thioredoxin (Trx) for their regeneration and do not react with or glutaredoxin, constituting a fifth class peroxiredoxins. The two chloroplastic Gpxs display marked selectivity toward electron donors, being exclusively specific Trxs y type reduction. In contrast, are much less...
The plant mitochondrial thioredoxin (Trx) system has been described as containing an NADPH-dependent Trx reductase and o . In addition to the isoform, , plants are known contain several chloroplastic isoforms cytosolic h isoforms. We report here presence in mitochondria of a isoform (PtTrx 2) belonging group. Western blot analyses with proteins isolated from both poplar GFP fusion constructs indicate that PtTrx 2 is targeted mitochondria. recombinant protein, 2, shown be reduced efficiently...
Wine aroma strongly influences wine quality, yet its composition and evolution during the winemaking process are poorly understood. Volatile compounds that constitute traditionally divided into three classes according to their origin: grape, fermentation maturation aroma. We challenge this view with meta-analysis review of grape volatiles precursors from 82 profiling experiments. compiled a list 141 common quantitatively compared 43 them. Our work offers insight complex relationships between...
Flowers are essential but vulnerable plant organs, exposed to pollinators and florivores; however, flower chemical defenses rarely investigated. We show here that two clustered terpene synthase cytochrome P450 encoding genes (
Monoterpenes are important constituents of the aromas food and beverages, including wine. Among monoterpenes in wines, wine lactone has most potent odor. It was proposed to form via acid-catalyzed cyclization (E)-8-carboxylinalool during maturation. only reaches very low concentrations but its extremely odor detection threshold makes it an aroma compound. Using LC-MS/MS, we show here that content wines correlates with their estimate kinetic constant for slow formation from...
Furanocoumarins are specialized metabolites that involved in the defense of plants against phytophagous insects. The molecular and functional characterization genes their biosynthetic pathway is only partially complete. Many recent reports have described gene clusters responsible for biosynthesis plants. To investigate possible co-localization furanocoumarin pathway, we sequenced parsnip BAC clones spanning two different loci. We found previously identified this CYP71AJ3 CYP71AJ4, were...
Increased metabolism is one of the main causes for evolution herbicide resistance in weeds, a major challenge sustainable food production. The molecular drivers this are poorly understood. We tested here hypothesis that suitable context emergence could be provided by plant enzymes with high innate promiscuity regard to their natural substrates. A selection yeast-expressed cytochrome P450 well documented narrow broad when metabolizing substrates was competence. positive candidate assayed...
Summary Epoxide hydrolases ( EH s) are present in all living organisms. They have been extensively characterized mammals; however, their biological functions plants not demonstrated. Based on silico analysis, we identified At 1 (At3g05600), a putative Arabidopsis thaliana epoxide hydrolase possibly involved cutin monomer synthesis. We expressed yeast and studied its localization vivo . also analyzed the composition of from A. lines which this gene was knocked out. Incubation recombinant with...
Shikonin is a red naphthoquinone natural product from plants with high economical and medical values. The para-hydroxybenzoic acid geranyltransferase (PGT) catalyzes the key regulatory step of shikonin biosynthesis. PGTs Lithospermum erythrorhizon have been well-characterized used in industrial production. However, its perennial medicinal plant Arnebia euchroma accumulates much more pigment underlying mechanism remains obscure. Here, we discovered characterized different isoforms AePGTs....
Cytochromes P450 are enzymes that participate in a wide range of functions plants, from hormonal signaling and biosynthesis structural polymers, to defense or communication with other organisms. They represent one the largest gene/protein families plant kingdom. The manual annotation cytochrome genes genome Vitis vinifera PN40024 revealed 579 sequences, including 279 complete genes. Most sequences grapevine organized physical clusters, resulting tandem segmental duplications. Although most...
Within many species, confusions occur between infraspecific taxa, recurrent ecotypic differentiation and phenotypic plasticity. Subspecies are often debated when they supported only by slight morphological coupled to habitat variation. In this context we tested the evolutionary support of four contested subspecies Dianthus superbus. Based on 147 Eurasian accessions, our integrative study combines three complementary approaches: (i) Genetic differentiation, with neutral genetic markers, i.e....
MINI REVIEW article Front. Plant Sci., 21 May 2013Sec. Physiology https://doi.org/10.3389/fpls.2013.00151
Various redox compounds are known to influence the structure of gluten network in bread dough, and hence its strength. The cereal thioredoxin system (NTS), composed nicotinamide adenine dinucleotide phosphate (NADPH)-dependent reductase (NTR) (Trx), is a major reducing enzymatic that involved seed formation germination. NTS particularly interesting tool for food processing due heat stability broad range protein substrates. We show here barley capable remodeling weakening dough. Furthermore,...
A single rabbit has been immunized with a mixture of six different proteins: green fluorescent protein, ferredoxin:NADP oxidoreductase, NADPH:thioredoxin reductase, thioredoxin f , fructose bisphosphatase and cyclophilin. Individual IgGs were re‐purified from the serum through specific interaction on small columns generated by grafting antigen CNBr‐Sepharose matrix. The specificity isolated IgG was checked Western‐blot analysis. Purified fractions obtained all antigens except .